The invention discloses a fluorine-containing polymer film, and a preparation method and an application thereof. The fluorine-containing polymer film is prepared from raw material components includinga fluorine-containing polymer, a modification additive, a fluorine-containing polymer good solvent and a fluorine-containing polymer poor solvent through a steam-induced phase separation method, thefluorine-containing polymer film has the properties of a symmetric film or an asymmetric film, the microstructure of the fluorine-containing polymer film comprises compact, nodular and cellular pores,the porosity of the fluorine-containing polymer film is 20-85%, and the pore size range of the fluorine-containing polymer film is 150-3000 nm. The fluorine-containing polymer film can be a symmetricor asymmetric film, is adjustable in microstructure, surface performance, porosity and pore size, has an application prospect in the separation fields such as microfiltration, ultrafiltration, oil-water separation and emulsion oil-water separation, and can also be used for a battery diaphragm.