3 results about "Partridges" patented technology

The application belongs to the technical field of genetic breeding, and specifically discloses a method for constructing a pure line of ptilophogus percussor, which mainly utilizes microsatellite amplification, gene sequencing and typing, and family grouping after analyzing the genetic structure of a group; finally, after expansion, subculture, and two generations, the inbreeding coefficient is calculated, and if the inbreeding coefficient is less than 0.01 and the inbreeding increment of generations is less than 0.003, it is indicated that the construction of the pure line is successful. Compared with the prior art, the application can quickly homozygous ptilophogus percussor, keep the purity of the genetic resource variety, guarantee the genetic polymorphism of ptilophogus percussor, lay a foundation for genetic resource protection, effectively avoid the inbreeding depression of ptilophogus percussor, and lay a foundation for the development and utilization of ptilophogus percussor genetic resources and the cultivation of new matching lines.

This invention discloses a molecular biology method and its application for simultaneously detecting five poultry-derived components. Using primers shown in SEQ ID NO.1 and SEQ ID NO.2, genomic DNA of the sample to be tested is amplified by PCR. After capillary electrophoresis, sequence alignment is performed. A peak value of 234 bp indicates a chicken-derived component; a peak value of 271 bp indicates a quail-derived component; a peak value of 229 bp indicates a turkey-derived component; a peak value of 252 bp indicates a partridge-derived component; and a peak value of 223 bp indicates a pheasant-derived component. Peak values ​​of 234 bp, 271 bp, 229 bp, 252 bp, and 223 bp indicate the simultaneous presence of five poultry-derived components. The detection kit developed based on this method can generate considerable economic benefits and good social value.

The invention discloses a primer combination, a kit and a method for monitoring partridge Hainan based on environmental DNA, and belongs to the technical field of species molecular biology. In the application, two pairs of independent specific primers are adopted for double verification, so that the accuracy and specificity of detection can be improved, misjudgment caused by insufficient specificity of the primers or sample quality problem is effectively reduced, DNA of the Hainan mountain partridge is expected to be efficiently and accurately amplified from various environmental samples, and the detection accuracy and specificity of the Hainan mountain partridge are improved. Therefore, powerful technical support is provided for identification, population monitoring and protection and genetic diversity research of the Hainan mountain partridges.