Method for remarkably improving callus inductivity of iris pseudacorus L.

A callus induction and callus technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of reducing callus induction rate, prolonging callus induction time, callus material pollution, etc. To achieve the effect of high callus induction efficiency, good callus embryo property and high natural seed setting rate

Inactive Publication Date: 2015-10-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, suspension culture needs to use a shaker, which consumes energy, and the process is cumbersome. In the later stage of transgenic or rapid propagation operation, it is necessary to use nylon membrane to filter and transfer the large callus to a solid medium for standby. Too many intermediate processes are very difficult. May lead to contamination of callus material
In addition, there is dormancy in the mature seeds of Acorus japonica, which will seriously reduce the callus induction rate and prolong the callus induction time

Method used

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  • Method for remarkably improving callus inductivity of iris pseudacorus L.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Step 1: Material adoption:

[0031] Fifty days after the flowering period of the yellow iris, the well-developed capsules (about 8-10 cm in length) were taken, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 4 days, followed by subsequent operations.

[0032] Step 2: Callus induction medium configuration:

[0033] The callus induction medium is MS medium containing 30 g / L sucrose, 3 g / L Phytagel and other additives. The specific formula of the other additives is shown in Table 1, and the pH value of the medium solution is 5.85.

[0034] After the prepared medium solution is autoclaved at 121°C for 18 minutes, it is dispensed into autoclaved glass petri dishes on the ultra-clean workbench. After the medium is solidified, wrap it in plastic wrap or foil and place it on Store in a sterile environment for later use.

[0035] Step 3: Disinfect the explant:

[0036] Soak the frozen pods in step 1 in tap water with detergent and a small amount of TWEEN-20 for 18 min...

Embodiment 2

[0046] Step 1: Material adoption:

[0047] 40 days after the flowering period of the yellow iris, the well-developed capsules (about 7-10 cm in length) were taken, placed in a sealed bag and refrigerated in a refrigerator at 4°C for 5 days, followed by subsequent operations.

[0048] Step 2: Callus induction medium configuration:

[0049] The callus induction medium is MS medium containing 30g / L sucrose, 3g / L Phytagel, 0.2mg / L KT and 2.0mg / L 2,4-D, and the pH of the medium solution is 5.8.

[0050] After the prepared medium solution is autoclaved at 121°C for 15 minutes, it is dispensed into autoclaved glass petri dishes on the ultra-clean workbench. After the medium has solidified, wrap it in plastic wrap or foil and place it on Store in a sterile environment for later use.

[0051] Step 3: Disinfect the explant:

[0052] Soak the refrigerated capsules in step one in tap water with detergent and a small amount of TWEEN-20 for 15 minutes, then rinse them under running water for 0.5 hour...

Embodiment 3

[0060] Step 1: Material adoption:

[0061] At 50 days after the flowering period of the yellow iris, the well-developed capsules (about 8-10 cm in length) were taken, placed in a sealed bag and kept in a refrigerator at 4°C for 4 days, and then the follow-up operation was performed.

[0062] Step 2: Callus induction medium configuration:

[0063] The callus induction medium is MS medium containing 30g / L sucrose, 3g / L Phytagel, 0.2mg / L KT and 2.0mg / L 2,4-D, and the pH value of the medium solution is 5.85.

[0064] After the prepared medium solution is autoclaved at 121°C for 18 minutes, it is dispensed into autoclaved glass petri dishes on the ultra-clean workbench. After the medium is solidified, wrap it in plastic wrap or foil and place it on Store in a sterile environment for later use.

[0065] Step 3: Disinfect the explant:

[0066] Soak the refrigerated capsules in step one in tap water with detergent and a small amount of TWEEN-20 for 18 minutes, then rinse under running water for...

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Abstract

The invention relates to the technical field of cell engineering tissue culture, and aims to provide a method for remarkably improving the callus inductivity of iris pseudacorus L. The method comprises the following steps: taking materials, configuring a callus induction culture medium, disinfecting an explant, inoculating the disinfected explant, and inducing a callus and an embryonic callus. Adopting a tender embryo of iris pseudacorus L. to serve as the explant, the method has the characteristics of low pollution rate and high material availability, and has the outstanding advantages of being high in callus induction efficiency, low in explant tender viruses, simple and convenient to operate, and high in obtained callus embryo quality.

Description

Technical field [0001] The invention relates to the technical field of cell engineering tissue culture, in particular to a method for significantly improving the callus induction rate of Huangchangpu. Background technique [0002] Yellow Iris is a perennial herb of the genus Iris in the Iridaceae family. The flowering period is from April to June. The flower color is yellow and bright, the leaf is sword-shaped, and it is green and green. It has high ornamental value. In garden applications, yellow calamus is mostly used as emergent plants, but studies have shown that its ecological adaptability is strong, and it can also be cultivated in xerophytes and wet cultivation (Han Yulin et al., 2006). The yellow calamus is resistant to salt and alkali, and has a certain ability to absorb heavy metal ions and organic pollution in water. [0003] Yellow Iris belongs to diploid, and has stronger reproductive ability than other plants of Iris. Research on it as a model plant can provide a the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李丹青夏宜平张佳平李康任梓铭
Owner ZHEJIANG UNIV
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