Cow in-vitro fertilization embryo culture fluid and culture method thereof

An embryo culture medium and a culture medium technology, which is applied to the field of bovine in vitro fertilization embryo culture medium, can solve the problems of low efficiency, high production cost of in vitro fertilization embryos, and limited wide application, so as to reduce costs, accelerate the genetic breeding process, and improve development. effect of ability

Inactive Publication Date: 2014-07-02
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Although in vitro fertilization technology can be successfully applied to many mammals, the high cost and low efficiency of in vitro fertilized embryo

Method used

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  • Cow in-vitro fertilization embryo culture fluid and culture method thereof
  • Cow in-vitro fertilization embryo culture fluid and culture method thereof

Examples

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Embodiment Construction

[0020] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0021] The culture method of embodiment bovine in vitro fertilization embryo

[0022] 1. Reagents

[0023] Mature medium: containing 0.01IU / ml FSH, 10IU / ml LH, 1μg / ml estradiol, 100ng / ml IGF, 50ng / ml EGF, 100U / ml penicillin, 100μg / ml streptomycin, 10% fetal bovine serum TCM-199 culture medium.

[0024] Semen Wash: Contains 112.0mM NaCl, 4.02mM KCl, 2.25mM CaCl 2 2H 2 O, 0.52mM MgCl 2 ·6H 2 O, 0.83mM KH 2 PO 3 , 37.0mM NaHCO 3 , 1.25mM sodium pyruvate, 10μg / ml heparin, 4mg / ml bovine serum albumin (Bovine serum albumin, BSA), 10mM caffeine, 100U / ml penicillin, 100μg / ml streptomycin aqueous solution.

[0025] Semen fluid: Contains 1...

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Abstract

The invention provides a culture fluid exclusively used in cow in-vitro fertilization embryos. The culture fluid contains 109.0mM-110mM of NaCl, 2.9mM-3.1mM of KCl, 26.0mM-26.5mM of NaHCO3, 0.5mM-1.0mM of MgCl2.6H2O, 1.0mM-1.3mM of KH2PO3, 0.4mM of sodium pyruvate, 1.5 mM of glucose, 5mM of galacturonic calcium, 10v/v% fetal bovine serum, 1mM of L- glutamine, 2v/v% essential amino-acid, 1v/v% nonessential amino acid and 1mM-10mM of glutathione. The cow in-vitro fertilization embryos are placed into the culture fluid to carry out in-vitro cultivation, and results are shown to be obviously superior to those of a control group which is not added with GSH (glutathione), and therefore, developmental rate of blastula and embryo quality are improved, cost for producing embryos in vitro is lowered, experimental basis is provided for applying a cow IVF (in vitro fertilization) technology to practice, and a genetic breeding process can be greatly accelerated.

Description

technical field [0001] The invention belongs to the field of agriculture-animal husbandry and veterinary medicine, and in particular relates to a bovine in vitro fertilized embryo culture solution and a culture method. Background technique [0002] With the development of modern agricultural science and technology, in order to make full use of the reproductive potential of fine-bred cows and accelerate the process of genetic breeding, it is inevitable to apply efficient new breeding technologies in production practice. Ovum pick UP (OPU) and in vitro fertilization (In Vitro Fertilization, IVF) are new technologies for embryo engineering that developed rapidly in the 1980s. The combination of the two can obtain a large number of embryos with definite genetic pedigrees. thereby shortening the generation interval. At present, these two technologies have become important breeding technologies adopted by farmers in developed animal husbandry countries such as Europe, America and...

Claims

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Application Information

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IPC IPC(8): C12N5/073
Inventor 杜卫华孙尉俊朱化彬
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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