In-vitro culture system for Tibetan yak oocyte

A technology for in vitro culture of oocytes, applied in the field of Tibetan yak oocyte in vitro culture system

Pending Publication Date: 2018-09-28
TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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Problems solved by technology

BMP15 is one of the most important cytokines involved in the regulation of fo...

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  • In-vitro culture system for Tibetan yak oocyte
  • In-vitro culture system for Tibetan yak oocyte
  • In-vitro culture system for Tibetan yak oocyte

Examples

Experimental program
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experiment example 1B

[0025] Experimental example 1 Effect of BMP15 on maturation of yak granulosa cells and their oocytes in vitro

[0026] 【Objective】The purpose of this study was to investigate the effects of bone morphogenetic protein 15 (BMP 15) on the proliferation of yak granulosa cells (Granulosa Cells, GCs) and the culture of oocytes (oocytes, COCs) in vitro. [Method] The ovaries of healthy yaks were taken, and COCs and GCs were isolated for in vitro maturation culture; 0, 10, 50, 100 ng·mL were added to the yak maturation medium -1 After BMP15, the proliferation of yak GCs was detected by the CCK-8 method and the oocyte maturation rate was counted; then the downstream molecule Smad 4 of the signaling pathway and the cumulus expansion marker genes HAS 2 and PTX 3 in COC treated with BMP15 were detected by Real-time PCR method , the expression level of PTGS2. 【Result】Added 0, 10, 50, 100 ng·mL to the culture of yak GCs -1 After BMP15, the results showed that adding 100ng·mL -1 The cell p...

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Abstract

The invention relates to an in-vitro culture system for Tibetan yak oocyte. Researches show that in an in-vitro culture process of cumulus-oocyte complexes (COC) of yak, proliferation of granule cellsof the yak can be obviously improved by adding exogenous BMP15, and an effect of obviously improving the oocyte maturation rate is achieved. According to the culture system disclosed by the invention, the influence of the BMP15 on yak COC in-vitro culture and a molecular regulation mechanism thereof in an in-vitro maturation process can be explored, and a developmental mechanism of the yak oocytein the maturation process can be further known, so that the in-vitro maturation culture quality of the yak oocyte and the developmental capacity of early embryos can be improved.

Description

technical field [0001] The invention belongs to the field of animal reproduction science and technology, and in particular relates to an in vitro culture system of Tibetan yak oocytes. Background technique [0002] In vitro maturation of oocytes is one of the basic techniques of mammalian reproduction and is widely used in animal reproduction [1] . However, studies have found that the developmental ability of most mammalian oocytes matured in vitro is significantly lower than that of oocytes matured in vivo, so people continue to explore ways to optimize the culture system for in vitro maturation. Studies have found that many cytokines play different roles in follicular development and maturation [2] , such as transforming growth factor plays an important biological role in the development of mammalian follicles [3,4] , and have good biological effects at different developmental stages [5,6] . Bone morphogenetic protein 15, also known as growth differentiation factor 9B...

Claims

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Application Information

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IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/155
Inventor 徐业芬王玉恒牛家强索朗斯珠强巴央宗
Owner TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
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