6results about How to "Improve developmental ability" patented technology

The invention discloses an enhancer Enh483 related to bovine muscle development and application of the enhancer Enh483 in promoting bovine myoblast proliferation and / or differentiation. According to the method, existing ATAC-Seq data and Chip-seq data in the proliferation stage and differentiation stage of buffalo myoblasts are utilized to visually analyze chromatin open areas through IGV to predict annotation enhancers, candidate enhancers Enh483 related to muscle development are screened out, and the enhancers have enhancer activity detected by a dual-luciferase vector report system; after the activity of Enh483 is inhibited, the proliferation and differentiation of buffalo myoblasts can be inhibited, which indicates that Enh483 can promote the proliferation and / or differentiation of buffalo myoblasts. According to the invention, an important theoretical basis is provided for an action mechanism of transcription regulation elements such as enhancers in muscle development, and data support is provided for buffalo breeding and strain breeding.

The utility model provides an aircraft control assembly rapid troubleshooting test device, comprising a test host computer and a test circuit board, the test host computer sends a test control instruction to the test circuit board, and the test circuit board outputs a discrete signal for the test of a to-be-tested device to the to-be-tested device after receiving the test control instruction. And after the to-be-tested device receives the test discrete signal, test feedback information is transmitted to the test circuit board, and the test circuit board sends the test feedback information to the test upper computer, so that the test upper computer processes the test feedback information to judge whether the function of the to-be-tested device is normal or not. Compared with a traditional test bench test method, the method has the advantages that functions which cannot be realized by a traditional test bench can be realized by using existing software and hardware, the time for completing the test of a single aircraft control assembly is shorter, the test efficiency is higher, a more efficient and effective test program is realized, and the overall quality and reliability of the aircraft control assembly are improved.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

The invention discloses application of alpha-lipoic acid in enhancing animal embryo in-vitro developmental capacity and a method for enhancing animal embryo in-vitro developmental capacity, and belongs to the technical field of sheep embryo in vitro. The application comprises application of alpha-lipoic acid or pharmaceutical salt thereof in enhancing animal in-vitro embryo developmental capacity. The method has the advantages that from the perspective of oxidative stress, the developmental capacity, blastocyst rate and blastocyst quality of the sheep in-vitro embryo can be effectively improved by adding 5 mu M and 10 mu M ALA in the sheep in-vitro embryo culture process.

The present invention provides a bovine oocyte maturation fluid and application, belonging to the field of animal breeding technology. The present invention effectively improves the bovine oocyte maturation efficiency, promotes the proliferation of cumulus cells, improves the embryo development ability, and thus improves the bovine in vitro embryo production efficiency by adding lipoxin to the basic culture medium.

The invention provides a method for improving the transfer developmental capacity of a frozen bovine IVF (in-vitro fertilization) embryo, which is characterized in that in the processes of freezing and unfreezing the bovine IVF embryo, culturing the embryo in a later period and the like, high-concentration sodium pyruvate, insulin, TLR2 (toll-like receptor 2) and a Cytosporone B are independently or jointly added, so that the survival rate and developmental capacity of the frozen embryo, the pregnancy rate after transplantation and the like are improved. Experiments show that the embryo survival rate, the hatching rate and the embryo transfer pregnancy rate of a group which is jointly added with sodium pyruvate, insulin, TLR2 and Nur77 agonist B are obviously higher than those of a double-substance adding group, a single-substance adding group and a control group. The method lays a foundation for production and related scientific research of in-vitro fertilized embryos in the future, provides an effective, safe and feasible strategy for improving in-vitro embryo production efficiency and embryo quality, and has great popularization value.