Reagent and method for increasing sperm movement speed

A technology of movement speed and reagents, which is applied in the field of reproductive medicine, can solve the problems of no improvement of sperm, and achieve the effect of improving sperm motility, less ROS, and improving activity

Inactive Publication Date: 2015-12-16
SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are no related reagents and methods to increase the speed of sperm movement to improve the fertilization ability of sperm

Method used

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  • Reagent and method for increasing sperm movement speed
  • Reagent and method for increasing sperm movement speed
  • Reagent and method for increasing sperm movement speed

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Objects and methods

[0033] 1.1 Semen sample selection

[0034] According to the standards of the fifth edition of the "World Health Organization Human Semen Analysis Laboratory Technical Manual" and through medical history inquiry, physical examination, semen etiological examination, etc., semen parameters were selected within the normal range, and prostatitis, varicocele and other related diseases were excluded. Disease samples. 20 semen samples were selected, and the age range was 30.8±3.11 years old.

[0035] 1.2 Semen sample processing and experimental grouping

[0036] The sperm culture medium was HTF culture medium supplemented with 10% human serum albumin (HTF was purchased from Origio Company, product number: ART-1020). After the semen was liquefied, it was directly diluted with the sperm culture medium, which was the pre-capacitation group. The upstream method uses 1 ml of sperm culture medium, spread on top of the liquefied semen sample, and upstream ...

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Abstract

The invention relates to a reagent and method for increasing the sperm movement speed. The reagent is prepared from progesterone, N6-Monobutyryladenosine-3',5'-cyclic monophosphate and Calcium Ionophore A23187, wherein the concentration ratio of progesterone to N6-Monobutyryladenosine-3',5'-cyclic monophosphate to Calcium Ionophore A23187 is (10-100 microgram/ml):(1-10mM):(1-10mM). The method includes the steps that a sperm culture solution is laid flat on a liquefied semen sample, and after 0.8-1.2 h of upward swimming, liquid at the uppermost end is extracted to obtain capacitated sperms; then, a sterile straw is filled with a sperm culture solution, the reagent is added into the top, the sterile straw is inserted into the upper portion of the capacitated sperms, and after 0.8-1.2 h of upward swimming, liquid at the upper end is collected. By means of the method, the sperm movement speed can be greatly increased, and a theoretical basis is provided for increasing the in-vitro fertilization rate or improving the embryo quality.

Description

technical field [0001] The invention relates to the technical field of reproductive medicine, in particular to a reagent and a method for increasing the movement speed of sperm. Background technique [0002] Although human testicles can use spermatogenic stem cells to produce hundreds of millions or even an infinite number of sperm, and each ejaculation will also ejaculate millions of sperm, but these sperm are not all the same. The differences among sperm are manifested in the difference of epididymis protein, the integrity of nuclear DNA, the number and mutation of mitochondrial DNA (mtDNA), the distribution of lipids and sugar groups in cell membrane, the integrity of acrosome structure and many other aspects. In the body, the number of sperm that can reach the fertilization site (ampulla of the fallopian tube) is only about 20 to 200. Only this type of sperm can finally fertilize the oocyte, while the function of most other sperm is immunosuppressive. The molecular comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076C12N5/073
Inventor 韩毅冰张晔范宇平滕晓明
Owner SHANGHAI FIRST MATERNITY & INFANT HOSPITAL
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