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Safety detection method for culture oil for assisted reproductive technology

A detection method and assisted reproduction technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, detection of programmed cell death, etc., can solve problems affecting the quality of mineral oil detection, and achieve improved quality control standards and Sensitivity, improved quality, and increased efficacy in vitro growth and development

Inactive Publication Date: 2019-07-05
东蕴医疗科技(上海)有限公司
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Problems solved by technology

However, human or animal serum proteins in the culture fluid (culture droplets) have the function of adsorbing organic harmful substances, which affects the detection of mineral oil quality
[0004] Therefore, the potential toxicity of many mineral oils cannot be detected by conventional MEA methods. Through clinical verification, the applicant found that the sensitivity of the existing MEA methods still needs to be improved in order to detect the safety of mineral oils more accurately and sensitively, ensuring its Safety and reliability of use

Method used

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  • Safety detection method for culture oil for assisted reproductive technology
  • Safety detection method for culture oil for assisted reproductive technology

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Embodiment 1

[0028] step:

[0029] 1. Female mice (C57Bl, Nanjing Jicui Yaokang) aged 5-7 weeks were intraperitoneally injected with 5 IU pregnant mare serum (PMSG, Sigma-Aldricn, G4877) for superovulation. After 48 hours, 5 IU of human chorionic gonadotropin (hCG, Sigma-Aldrich, C1063) was used to induce ovulation, and the female and male mice were mated and reared in a single cage. After 18-2 hours, confirm successful mating by checking the female for the presence of a vaginal plug. 1-cell mouse embryos were collected from the oviductal ampulla, and granulosa cells were removed with 1 mg / ml hyaluronidase.

[0030] Collection of 1-cell mouse embryos: 20 hours after hCG injection, female mice were killed by neck dislocation, and 1-cell mouse embryos were collected in the ampulla of oviduct. Place the collected flocculent fertilized ovum into hyaluronidase (1 mg / mL), and take out immediately after the granulosa cells around the embryo are digested and separated (usually 1 minute). After ...

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Abstract

A safety detection method for culture oil for the assisted reproductive technology is carried out according to the internationally common 1-cell embryo method; MEA (mouse embryo assay) employs a mouseembryo in-vitro conventional culture system; upon microdroplet culture of 1-cell mouse embryos, 0.01 mg / mL to 0.03 mg / mL of PVA is added to replace the original human serum protein or animal serum protein, and potential toxicity of mineral oil can be detected by the MEA method more efficiently; the safety detection method is more sensitive at the premise of invariable blastocyst rate standard, and safety control of the industry for the products is enhanced.

Description

technical field [0001] The invention relates to a method for detecting the safety of oil used for assisted reproductive culture, and belongs to the field of quality control and detection of biological products. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. At present, with the development of society, human assisted reproductive technology (Assisted Reproductive Technology, ART) is highlighting its importance. The safety of human assisted reproductive technology is an important guarantee related to the normal development of gametes and embryos, clinical results and long-term health of offspring. During embryo culture, culture droplets (50 μL-100 μL) need to be covered with mineral oil for in vitro embryo culture. This technology can further increase the sensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCG01N33/5005G01N2500/10
Inventor 尹航莫贝克胡彦新徐进徐卫良
Owner 东蕴医疗科技(上海)有限公司
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