14 results about "Cells embryo" patented technology

The application provides sgRNA which specifically targets the Rosa26 safe site of a cashmere goat and application of using CRISPR / Cas9 to complete site-directed knockout and site-directed integration of an EGFP gene. The application firstly uses a bioinformatics method to predict the complete sequence of the Rosa26 site, then designs two sgRNA aiming at the site, constructs a targeting vector based on the CRISPR / Cas9 system, verifies the guiding efficiency of the sgRNA, simultaneously constructs a homologous integration EGFP gene vector, co-transfects the sgRNA and the homologous integration vector into cashmere goat fetal fibroblasts, and obtains a cell strain of the Rosa26 site site-directed integration of the EGFP gene. The efficiency of the sgRNA of the application in specifically guiding Cas9 to cut the Rosa26 site reaches about 40%, effectively reduces the off-target phenomenon existing in the CRISPR / Cas9 system, and further reduces the mutation of non-target gene sequences caused by non-specific cutting. The Cas9 / gRNA expression vector can realize the specific knockout or knock-in of the Rosa26 site at the cell, embryo or even individual level, so as to study the expression of specific genes and provide technical support for the cultivation of new goat breeds.

The invention belongs to the technical field of plant tissue culture, and particularly discloses a somatic embryogenesis and plant regeneration method for ailanthus altissima. The method comprises the following steps: (1) taking a leaf axis, a small leaf or a compound leaf of an ailanthus altissima aseptic seedling as an explant, inoculating the explant on an embryogenic callus induction culture medium, and culturing to form an embryogenic callus; (2) inoculating the embryogenic callus obtained in the step (1) to a somatic embryo induction culture medium for culturing; and (3) selecting embryogenic calluses with good growth vigor and somatic embryos on the surfaces, and subculturing the embryogenic calluses in a somatic embryo germination culture medium to obtain somatic culture seedlings. According to the invention, a somatic embryo regeneration system of Ailanthus altissima is established for the first time, and the problem that the embryonic callus and somatic embryo of Ailanthus altissima are difficult to induce is effectively solved.

The invention discloses a method for screening negative sorghum somatic embryos. The method comprises the following steps: (1) disinfecting mature sweet sorghum seeds; (2) preparing a first 2, 4-D concentration gradient callus induction culture medium; (3) sowing the sweet sorghum seeds into a first 2, 4-D concentration gradient callus induction culture medium; (4) sealing, carrying out dark culture, and screening out type 2 embryogenic calluses; (5) preparing a second 2, 4-D concentration gradient callus induction culture medium; (6) subculturing the 2-type embryogenic callus to a second 2, 4-D concentration gradient callus induction medium, sealing, carrying out dark culture, and screening out 2-type yellow granular callus; (7) preparing a differential medium; (8) differentiation culture; (9) sealing, controlling the photoperiod to be 16h / 8h, and continuously culturing; (10) preparing a rooting culture medium; (11) rooting culture; (12) sealing, rooting after continuous culture, and transplanting strong roots into soil to grow into complete fertile plants.

The invention discloses a construction method of a Dcaf17 gene modified golden hamster model and application of the Dcaf17 gene modified golden hamster model. The method comprises the following steps: under a red light source, injecting a Cas9 functional element and sgRNA of a targeted Dcaf17 gene exon 1 through a two-cell embryo, and transplanting the embryo into a receptor body, so as to obtain the Dcaf17 gene modified golden hamster. The Dcaf17 gene modified golden hamster model is constructed by utilizing two-cell stage embryo gene editing, and the Dcaf17 gene modified golden hamster homozygous male rat has the phenotype characteristics of no fertilization ability, normal testis and the like, and is used for replacing ligation male rats to prepare false pregnancy receptor rats. The receptor can efficiently identify and utilize a false pregnancy receptor through microscopic examination of sperms by a microscope. Therefore, the invention solves the technical difficulty of receptor preparation in related experiments of assisted reproduction of the golden hamster, and can be used for phenotype research of the Dcaf17 gene and related applications of assisted reproduction of the golden hamster.

1. Name of the design product: stainless steel liquid nitrogen tank. 2. Use of the design product: stainless steel liquid nitrogen tank for storing biological samples such as biological cells, embryos, blood bags, etc. 3. Design points of the design product: in shape. 4. Picture or photo that best indicates the design points: perspective view.

The invention discloses a GhRCAR3 gene and application of the GhRCAR3 gene in acceleration of embryogenic callus differentiation of cotton. The nucleotide sequence of the GhRCAR3 gene is as shown in SEQ ID NO. 3. The overexpressed GhRCAR3 gene disclosed by the invention can be used for rapidly inducing the callus to be differentiated into the embryonic callus. Therefore, the GhRCAR3 gene plays an important role in the aspect of somatic embryogenesis of cotton. Therefore, overexpression of the gene and application of the gene to commercial cotton varieties difficult in genetic transformation are beneficial to acceleration of the callus induction process, technical support is provided for cultivation of high-quality cotton germplasm with high yield and stress resistance, and the gene is suitable for popularization and application.

1. The name of the design product: aluminum alloy liquid nitrogen tank. 2. The use of the design product: aluminum liquid nitrogen tank for storing biological cells, embryos, blood bags and other biological samples. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

Genetically modified mice and methods for making an using them are provided, wherein the mice comprise a replacement of all or substantially all immunoglobulin heavy chain V gene segments, D gene segments, and J gene segments with at least one light chain V gene segment and at least one light chain J gene segment. Mice that make binding proteins that comprise a light chain variable domain operably linked to a heavy chain constant region are provided. Binding proteins that contain an immunoglobulin light chain variable domain, including a somatically hypermutated light chain variable domain, fused with a heavy chain constant region, are provided. Modified cells, embryos, and mice that encode sequences for making the binding proteins are provided.

The application relates to a high-efficiency induction method of liquorice adventitious roots and complete plants, and relates to the technical field of plant regeneration, which comprises the following steps: placing liquorice stem cells or liquorice somatic embryos in an induction culture medium to obtain liquorice complete plants or liquorice adventitious roots, wherein the culture medium is an MS culture medium containing a hormone composition composed of 6-BA and KT or an MS culture medium containing a hormone composition composed of NAA and IBA. The application has high induction efficiency, and the quality of the regenerated products prepared by the method is excellent.

The invention discloses a method for improving the embryogenic ability of cedarwood based on 5-azacytidine, and belongs to the technical field of plant biology. The method comprises the following steps: carrying out dark culture on cunninghamia lanceolata embryogenic calluses in a proliferation culture medium containing 5-azacytidine; carrying out dark culture on the calluses in an induction culture medium to induce cotyledon cell embryos; and finally, carrying out illumination culture on the somatic embryos in a germination culture medium. According to the method, 5-azacytidine with a specific concentration is exogenously added in explant proliferation and induction stages, so that the cell DNA methylation level can be remarkably reduced, and the embryogenic state of the callus is effectively maintained. Practice shows that the method can significantly improve the proliferation efficiency (up to 2.6 times) and the somatic embryo occurrence rate (up to 2.9 times) of cunninghamia lanceolata calluses of different genotypes, and an effective scheme is provided for solving the technical problem that the somatic embryogenesis capacity of cunninghamia lanceolata declines.

The invention discloses a method for promoting regeneration of somatic embryos of sweet sorghum. The method comprises the following steps: (1) disinfecting mature sorghum seeds; (2) preparing a culture medium with a first 2, 4-D concentration gradient; (3) sowing sorghum seeds on a first 2, 4-D concentration gradient culture medium, carrying out dark treatment for 1-5 weeks, and inducing to obtain calluses; (4) preparing a culture medium with a second 2, 4-D concentration gradient; and (5) subculturing the callus to the second 2, 4-D concentration gradient culture medium prepared in the step (4), carrying out photoperiod of 16h / 8h and treatment time of 1-5 weeks, and obtaining the type 2 embryogenic callus at the hypocotyl after culture. The method can efficiently and stably promote the regeneration of the somatic embryos of the sweet sorghum, and overcomes the defects of long preparation period and high cost of the immature embryos of the sorghum; the problem of annual supply of sorghum gene editing receptors is solved.

The instant disclosure provides multiple precision edits of bovine integrin beta 2 (CD18) that confer reduced susceptibility to Manheimia haemolytica leukotoxin A (LktA). Further disclosed are methods of producing genome-edited bovine cells, embryos, and animals comprising a modified CD18 incorporating the precision edits.

The application provides a method for regulating the pluripotency state of human embryonic stem cells and / or activating 8-cell-like cells NELFA The application belongs to the technical field of genetic engineering and provides a method for regulating the pluripotency state of human embryonic stem cells and / or activating 8-cell-like cells NELFA The application provides a method for regulating the pluripotency state of human embryonic stem cells. NELFA The application successfully discloses and verifies the role of the key positive regulator in driving the conversion of human embryonic stem cells into early embryonic stage cells, and provides a new technical means for efficiently obtaining primitive stem cells and 8-cell embryo-like cells, and then obtaining seed cells with expanded pluripotency.