Method for efficiently establishing pure line gene knock-out mouse model

Abstract

The invention provides a kind of transgenic inhuman mammal by producing klone gene without mouse model. Roll outside self murder gene into the mammal. The expression box contains promoter, self murder gene relation with promoter and termination codon. The natural masculine gene will express in testes organize after transgenic animal builds system. The transgenic animal can provide blastosphere for eliminating gene technic to improve the efficiency of the technic and to solve the problem that mouse genetic background is impure.

Description

technical field

[0001] The invention relates to the field of transgenic technology. More specifically, it relates to a pure line gene knockout mouse model, a method for establishing the pure line gene knockout mouse model, and uses of the pure line gene knockout mouse model. Background technique

[0002] Since the advent of gene knockout technology, it has been widely used in life science research. The gene knockout technology itself is also constantly developing, and conditional gene knockout techniques such as developmental stage specificity, tissue type specificity and drug induction have emerged, which greatly facilitate the detailed study of gene function. Gene knockout technology has become one of the most important means of gene function research.

[0003] Currently, there are three main ways to obtain gene knockout mice from homologous recombination cells.

[0004] One is to carry out gene targeting at the ES cell level to obtain homologous recombination ES cell c...

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