Blastocyst nutrient solution and preparation method thereof

A technology for culture medium and blastocyst, which is applied in the field of blastocyst culture medium and its preparation, can solve the problems of high cost, poor performance of solution effectiveness, etc., and achieves strict detection, inhibition of bacterial growth and operation. Simple and reasonable effect

Active Publication Date: 2014-11-12
山东威高瑞生医疗器械有限公司
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Problems solved by technology

[0003] At present, the blastocyst culture medium in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorated and continues to be used, it will cause irreversible damage to the experiment or clinical operation. the result of

Method used

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  • Blastocyst nutrient solution and preparation method thereof

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Embodiment Construction

[0029] The present invention will be further described below by way of examples.

[0030] 1. Formula and preparation method of blastocyst culture medium

[0031] The formula of table 1 blastocyst culture medium

[0032]

[0033] Note: The phenol red in the formula is "0", which means that the blastocyst culture medium does not contain phenol red; the human serum albumin is "0", which means that human serum albumin is not added before subpackaging, but added when using.

[0034] The preparation method of blastocyst culture fluid of the present invention comprises the following steps:

[0035] (1) Preparation of base fluid

[0036] a. First weigh each component according to the proportion and place them separately;

[0037] b. Dissolve the weighed components (except for bactericide, selective indicator, sodium bicarbonate and human serum albumin) in ultrapure water. 3 the principle of last access;

[0038] c, add weighed bactericide, indicator and sodium bicarbonate sele...

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Abstract

The invention discloses a blastocyst nutrient solution and a preparation method thereof. The blastocyst nutrient solution comprises a composition for treating and culturing a zygote, a bactericide, a selectively added indicator and a selectively added human serum albumin. Each index of the blastocyst nutrient solution reaches the standard of an in-vitro nutrient solution, the use is safe, and whether the nutrient solution is deteriorated or not can be easily determined, and therefore a powerful guarantee is provided to the assisted reproductive technology.

Description

technical field [0001] The invention relates to artificial assisted reproductive technology, in particular to a blastocyst culture solution and a preparation method thereof. Background technique [0002] Blastocyst culture fluid has been widely used in the development and culture of embryos in test-tube baby technology. The purpose of the blastocyst culture medium is: first, to provide nutrients for the survival and development of the embryo; second, to provide the necessary environment for the survival of the embryo. The use of blastocyst culture medium: In the laboratory or clinic, in the carbon dioxide environment, it provides nutrients and necessary environment for the division and development of blastocysts in vitro. [0003] At present, the blastocyst culture medium in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
Inventor 王维华吕汝举刘美静庄巍王英超
Owner 山东威高瑞生医疗器械有限公司
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