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Hyaluronidase and its preparation method

A technology of hyaluronidase and magnesium sulfate, applied in the direction of germ cells, vertebrate cells, animal cells, etc., can solve the problems of high cost, poor performance of solution effectiveness, etc., and achieve strict detection and preparation The process operation is simple and reasonable, avoiding the effect of unknown factors

Inactive Publication Date: 2018-03-16
SHANDONG WEGO NEW LIFE MEDICAL DEVICES
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Problems solved by technology

[0003] At present, the hyaluronidase in assisted reproductive technology in my country relies on imported finished products, the cost is high and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorated and continues to be used, it will cause irreversible damage to the experiment or clinical operation. the result of

Method used

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  • Hyaluronidase and its preparation method

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Embodiment Construction

[0026] The present invention will be further described below by way of examples.

[0027] One, hyaluronidase formula and preparation method

[0028] Table 1 Hyaluronidase formulation

[0029]

[0030] The preparation method of hyaluronidase of the present invention comprises the following steps:

[0031] (1) Preparation of hyaluronidase base solution

[0032] a. First weigh each component according to the proportion and place them separately;

[0033] b. Dissolve the weighed components (except bactericide, indicator, and sodium bicarbonate) in ultrapure water. During the dissolution process, follow the principle of first solid and then liquid, NaHCO 3 the principle of last access;

[0034] c, add weighed bactericide, indicator and NaHCO in the solution obtained in step b 3 ;

[0035] d. Detect the osmotic pressure and pH value of the solution. If the pH value is not within the range, adjust the pH value with prepared hydrochloric acid and sodium hydroxide solution, an...

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Abstract

The invention discloses hyaluronidase and a preparation method thereof. The hyaluronidase comprises a composition for treating ova, a bactericide and an indicator. Various indexes of the hyaluronidase meet the safe use standards of an in-vitro culture solution, whether the culture solution is deteriorated or not is easy to judge, and strong guarantee is provided for an assisted reproductive technology.

Description

technical field [0001] The invention relates to artificial assisted reproductive technology, in particular to a hyaluronidase and a preparation method thereof. Background technique [0002] Hyaluronidase on isolated cumulus has been widely used in IVF techniques. The purpose of hyaluronidase is to separate the cumulus surrounding the egg. The use of hyaluronidase: used for the separation of cumulus cells and eggs before egg microinjection. [0003] At present, the hyaluronidase in assisted reproductive technology in my country relies on imported finished products, the cost is high and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorated and continues to be used, it will cause irreversible damage to the experiment or clinical operation. the result of. Contents of the invention [0004] The purpose of the present invention is to provide a kind of hyaluronidase which is safe to use and easy to judge whether the culture medium has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
Inventor 王维华吕欣蓉刘美静吕汝举王啸林卢斌马一文田燕
Owner SHANDONG WEGO NEW LIFE MEDICAL DEVICES
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