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A kind of embryo culture mineral oil special for in vitro fertilization and preparation method thereof

A technology for embryo culture and in vitro fertilization, applied in biochemical equipment and methods, embryo cells, culture process, etc., can solve the problems of high cost, uneven quality of embryo culture mineral oil, product quality impact, etc., to achieve strict detection, Avoid the influence of unknown factors and have high safety effects

Active Publication Date: 2020-04-28
成都艾伟孚生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term dependence on imported finished products will not only lead to high costs, but also the quality of imported mineral oil for embryo culture is uneven, and it needs long-distance transportation, and unknown factors in the transportation process may also have a serious impact on product quality. If the quality of the product is poor Mineral oil, will cause irreversible results in experiment and clinical

Method used

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  • A kind of embryo culture mineral oil special for in vitro fertilization and preparation method thereof
  • A kind of embryo culture mineral oil special for in vitro fertilization and preparation method thereof
  • A kind of embryo culture mineral oil special for in vitro fertilization and preparation method thereof

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Effect test

Embodiment

[0031] 1. Preparation of Mineral Oil

[0032] (1) Preparation of reducing agent

[0033] Accurately weigh 2 g of ascorbic acid, place it in a beaker filled with 100 mL of ultrapure water, and stir it continuously with a glass rod at room temperature to dissolve.

[0034] Accurately weigh 2 g of cysteine, place it in a beaker filled with 100 mL of ultrapure water, and dissolve it with constant stirring with a glass rod at room temperature.

[0035] (2) preparation of sodium chloride solution

[0036] Accurately weigh 1.0 g of sodium chloride, place it in a beaker filled with 100 mL of ultrapure water, and stir it continuously with a glass rod at room temperature to dissolve it.

[0037] (3) Preparation of extractant

[0038] Take p204 as the extractant, sulfonated kerosene as the diluent of the extractant, and prepare the extractant p204 by diluting O / A at 4:6.

[0039] (4) Treatment of mineral oil for embryo culture in vitro fertilization

[0040] The specific operation p...

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Abstract

The invention discloses a mineral oil specially used for in vitro fertilization for embryo culture and a preparation method thereof. Light paraffin oil is used as a raw material, the extractant is diisooctyl phosphate, the solvent is ultrapure water, and ascorbic acid, cysteine, chlorine Mineral oil is obtained after purification of sodium chloride solution. The purification effect is remarkable, which can effectively reduce the endotoxin content, peroxide value and heavy metal content of the culture medium. The quality of the obtained mineral oil can reach or even exceed that of foreign products, avoiding the long-distance transportation The unknown factors brought about, and the purification process operation is simple and reasonable, and the detection is strict. It can provide fresh mineral oil for embryo culture for use in reproductive centers or scientific research institutes, and has a good market prospect.

Description

technical field [0001] The invention relates to the field of artificial assisted reproductive technology, in particular to a special mineral oil for embryo culture in vitro fertilization and a preparation method thereof. Background technique [0002] In Vitro Fertilization (In Vitro Fertilization) refers to the technology in which human sperm and eggs complete the fertilization process in an artificially controlled environment outside the body. It is abbreviated as IVF in English. Since the world's first "test-tube" baby was born in the UK on July 25, 1978, as of July 1, 2012, 5 million "test-tube" babies have been born. Covering culture medium with mineral oil has been used for long-term in vitro culture of mammalian fertilized eggs and embryos. Nowadays, embryos produced in vitro are usually cultured with microdrops or four-well plates covered with paraffin oil. This technology can use a small amount of culture medium for embryo culture to avoid The liquid evaporates. At...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10G53/04C12N5/073
CPCC10G53/04C10G2300/201C12N5/0604C12N2500/30
Inventor 米乐戴甄贾晓伟王瞿波
Owner 成都艾伟孚生物科技有限公司
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