5results about How to "Reduce endotoxin" patented technology

The invention belongs to the technical field of biology, and relates to a method for large-scale preparation of high-purity hydrolyzed corac acid or a salt thereof. The method comprises the following steps: 1) adding a solution containing corac acid or a salt thereof into a corac acid degrading enzyme containing an amino acid sequence as shown in SEQ ID NO: 1 or having at least 90% sequence identity with the SEQ ID NO: 1; terminating in the step 2) is achieved through a terminating agent, a boiling water bath and / or an ice bath, the terminating agent is selected from one or more of activated carbon, absolute ethyl alcohol, methyl alcohol, EDTA, trichloroacetic acid and sodium carbonate, and preferably, the activated carbon is used for achieving terminating in the step 2); and further obtaining the hydrolyzed corac acid or the salt thereof. The molecular structure of the hydrolyzed sodium currate prepared by the method provided by the invention has acetylation and pyruvic acid modification, the preparation condition is mild, the production cost is low, continuous production can be realized, and industrial amplification is easy.

The invention provides a drug carrier based on N-acetylglucosamine, a drug preparation and a preparation method. The drug carrier based on N-acetylglucosamine comprises N-acetylglucosamine and a cross-linking agent, the cross-linking agent is a compound with an aldehyde group or a ketone group, and the aldehyde group or the ketone group reacts with the deacetylated N-acetylglucosamine to generate one or more of a hydrazide bond, a hydrazone bond or an imine bond. The drug carrier based on N-acetylglucosamine shows remarkable advantages in the aspects of particle size distribution, drug loading capacity, encapsulation efficiency and cumulative drug release rate.

The invention discloses a CHO (Chinese hamster ovary) cell and a suspension process method, which are characterized in that a stable high-expression cell strain is obtained by improving a target expression vector, and the protein expression quantity of 1g / L is realized. The improved CHO cell can be suitable for a WAVE wave bioreactor and serum-free culture, contamination is effectively prevented, interference of exogenous factors is reduced, the level of related impurities such as endotoxin in a cell expression product is reduced, the operation steps are simplified, the occupied culture space is reduced, meanwhile, the culture time is shortened, and the culture efficiency is improved. Therefore, the culture efficiency is improved, bacterial contamination is effectively prevented, the endotoxin level in the product is reduced, and the production cycle is greatly shortened.

The invention discloses a composition containing sodium hyaluronate and DNA sodium and application of the composition, and belongs to the technical field of biology. The composition is prepared from the following raw materials: DNA sodium, sodium hyaluronate and mannitol, the preparation method of the DNA sodium comprises the following steps: adding a tissue into alkali liquor, and grinding to prepare slurry; adding an enzyme solution into the slurry for enzymolysis to obtain an enzymatic hydrolysate; adding HCl into the enzymatic hydrolysate, carrying out ultrasonic treatment, centrifuging, and taking supernate; and carrying out ultrafiltration on the supernatant, and drying the filtrate to obtain the DNA sodium. The endotoxin of the prepared DNA sodium is lower than 0.5 EU / mg, compared with the prior art, the safety is better, the composition is compounded by the sodium hyaluronate, the mannitol and the DNA sodium, on one hand, the stability of the DNA sodium in a liquid state can be improved, on the other hand, the anti-aging effect of the DNA sodium can be greatly improved, and a technical support is provided for application of the DNA sodium in the field of medical beauty.