50results about How to "Reduce endotoxin" patented technology

The invention discloses a swine straw feed and its preparation method. The swine straw feed is mainly prepared by mixing raw materials of corn straw, wheat straw 30-40 and Chinese herbal medicines. The formula provided by the invention has an advantage of reasonable collocation of proportions of proteins in corn straw, wheat straw, corn and the like, can basically satisfy the growth need of pigs,and can be used to increase income of the vast majority of peasants and improve the quality of pork. As the formula contains traditional Chinese medicines of cortex acanthopanacis, Chinese chive seedand the like, the formula has an effect of supplementing the center and boosting qi and can be used to increase disease resistance of pigs. By the adoption of the formula, pigs have bright furs and agood state during the feeding process. Pteridium aquilinum and Gynura Bicolor both belongs to edible wild herb, have an effect of heat clearing and detoxifying, and can minimize endotoxin in pigs andraise immunity and disease resistance. Galagal rhizome and haw have a function of strengthening stomach and can guarantee a good appetite for pigs. Peach leaves have laxation and insecticidal effectsand can be used to prevent pigs from being infected with diseases. The formula provided by the invention is reasonable and scientific.

The invention provides eye drops and a preparation method thereof, and relates to the eye drops. The invention provides the eye drops which have the advantages of shorter action time, lower cost, better effect and capacity of resisting corneal neovascularization and quickly repairing epithelium compared with the conventional eye drops, and a preparation method thereof. The eye drops comprise the following components in percentage by volume: 0.1 to 5 percent of bovine serum, 5 to 15 percent of thickener, 1 to 5 percent of pH regulation solution, 0.1 to 2 percent of osmotic pressure buffer, 0.5to 2 percent of antibiotic, 5 to 30 percent of original solution of kallikrein-binding protein (SA3K) and the balance of balanced salt solution. According to a prescription of the eye drops, the preparation method comprises the following steps of: adding the thickener, the bovine serum, the antibiotic and the original solution of SA3K into the balanced salt solution; uniformly mixing; regulating the pH value to be 7.2 to 7.4 by using the pH regulator; regulating the osmotic pressure to be 350 to 380mOsm / L by using the osmotic pressure buffer; and filtering and degerming by using a membrane toobtain the SA3K eye drops.

Chimeric somatostatin-based polypeptides, polynucleotides used to encode the polypeptides, the methods for isolating and producing the polypeptides and the uses thereof are provided. In addition, low cost adjuvants for enhanced immunogenic response are provided. Vaccinations that include both chimeric somatostatin-based polypeptides and novel adjuvants are included, useful in facilitating farm animal productivity.

Chimeric somatostatin-based polypeptides, polynucleotides used to encode the polypeptides, the methods for isolating and producing the polypeptides and the uses thereof are provided. In addition, low cost adjuvants for enhanced immunogenic response are provided. Vaccinations that include both chimeric somatostatin-based polypeptides and novel adjuvants are included, useful in facilitating farm animal productivity.

The invention discloses application of chitosan oligosaccharide as regulating agent for regulating animal intestinal flora disturbance, the chitosan oligosaccharide, a mixture containing the chitosanoligosaccharide and corresponding application. The polymerization degree of the chitosan oligosaccharide is 2 to 20, so that the intestinal flora disturbance caused by obesity or diabetes or an unreasonable diet structure can be regulated, growth of beneficial bacteria such as Akkermansia, Bifidobacterium and Lactobacillus of an intestinal tract is promoted, and proliferation of harmful bacteriasuch as Desulfovibrio and Helicobacter is obviously inhibited. The invention provides an application method of a clinically-acceptable medicinal preparation or health-care food which is made by usingthe chitosan oligosaccharide alone or a composition prepared from the chitosan oligosaccharide and the beneficial bacteria according to a conventional process. By utilizing the chitosan oligosaccharide, prevention and control of the intestinal disturbance caused by the diabetes, the obesity and the inflammatory bowel disease have an active application value.

The present disclosure provides a polymer comprising a derivative of chitosan, wherein the derivative is zwitterionic, as well as methods of using the polymer. In addition, the present disclosure provides a nanoparticle structure comprising a derivative of chitosan and a dendrimer, as well as methods of utilizing the nanoparticle structure.

The invention discloses eye drops and a preparation method thereof. The eye drops for treating keratonosus comprise the following components in percentage by volume: 0.1-5 percent of bovine serum, 5-15 percent of a thickening agent, 1-5 percent of a pH regulating agent, 0.5-2 percent of antibiotics, 5-20 percent of recombinant Nodinhibit-1 protein, and the balance of balanced salt solution. The preparation method of the eye drops for treating keratonosus comprises the following steps: uniformly mixing the thickening agent, the bovine serum, the antibiotics and the recombinant Nodinhibit-1 protein in the balanced salt solution, regulating the pH value to be 7.2-7.4 with the pH regulating agent, and regulating the osmotic pressure to be 350-380mOsm / L with an osmotic pressure buffering agent; and performing membrane filtration and sterilization to obtain the Nodinhibit-1 eye drops. The method is simple and reliable, and is easy to implement; the eye drops are simple in components, relatively low in cost and convenient to prepare. The eye drops do not contain hormone components, and can be used for a long time, so that occurrence of complications and sequelae in the administration period can be greatly reduced; bovine serum is added, so that adverse responses caused by using other serums can be remarkably reduced.

The invention discloses an eyedrop which has the pH value of 7.2-7.4 and comprise the following components in percentage by volume: 10%-15% of UNC5B recombinant protein, 0.1%-5% of bovine serum, 5%-15% of a thickener, 1%-5% of a pH regulator and 0.5%-2% of antibiotics; a solvent is a balanced salt solution. A preparation method of the eye drops is simple, reliable and easy to perform; the eye drops are simple in component, relatively low in cost and convenient to prepare.

The present invention provides a liquid for preserving cornea for medium period and a preparing method thereof, and relates to a liquid for cornea material. The invention provides the liquid for preserving cornea for medium period and the preparing method thereof, wherein the liquid has the advantages of longer storing time, higher quality, no pollution and relatively lower cost compared with theprior liquid for preserving cornea for medium period. The liquid for preserving cornea for medium period contains culture solution, thickening agent, bovine serum, amino acid, antibiotic, acid-alkalimodifying agent and cell nutrition components. According to the formula of liquid for preserving cornea for medium period, the thickening agent, the bovine serum, the amino acid, the antibiotic and cell nutrition components are added into the culture solution for mixing to uniform. The pH value is adjusted to 7.2-7.4 with the acid-alkali modifying agent. The osmotic pressure is adjusted to 350-380mOsm / L with an osmotic pressure buffering agent. The liquid for preserving cornea for medium period is obtained after membrane filtering for sterilizing.

The invention discloses an application of Lactobacillus rhamnosum LRX-01 in preparation of a product for preventing and treating human or mammal diseases caused by high temperature. When a heat stress test is carried out on a mouse, the inventor of the invention finds that the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged by pretreating the mouse with the Lactobacillus rhamnosum LRX-01, and the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged by treating the mouse with the Lactobacillus rhamnosum LRX-01, so that the survival time of the mouse subjected to high-temperature treatment can be obviously prolonged, and the application of the Lactobacillus rhamnosum LRX-01 in preparation of the product for preventing and treating the human or mammal diseases caused by high temperature can be obviously prolonged. The lactobacillus rhamnosus LRX-01 has the advantages that the lactobacillus rhamnosus LRX-01 can be used for preventing and treating human or mammal diseases caused by high temperature, the serum inflammatory factors and endotoxin of the lactobacillus rhamnosus LRX-01 can be reduced, the intestinal injury caused by heat stress can be relieved, and the results show that the lactobacillus rhamnosus LRX-01 has potential application value in the aspect of preventing and treating the human or mammal diseases caused by high temperature.

The invention relates to a composite bacterial cellulose dressing based on a controllable nanofiber space structure and a preparation method thereof. The preparation method comprises the steps of: firstly, dropwise adding gelatin-based aqueous solution into a non-woven material containing a protein component; after drying, dropwise adding culture bacterium solution onto the non-woven material; until the composite non-woven material adsorbs the culture bacterium solution to be saturated, adding fermentation culture solution to carry out standing culture; and finally, immersing a compound obtained by standing culture into triton aqueous solution with the concentration of 0.1 to 1wt% for purification, and drying to obtain the non-woven material composite bacterial cellulose dressing. The prepared dressing has a density structure, specifically, bacterial cellulose nanofibers are distributed on non-woven micron-sized fibers, and the distribution and the density degree of the nanofibers areadjustable. The prepared non-woven material composite bacterial cellulose dressing prepared by the invention has good biocompatibility, mechanical properties and wound exudate absorption performance,and has huge application prospects in the biomedical fields of a wound dressing, a human body repair material, a tissue engineering material and the like.

The invention provides a purifying method of medium molecular weight hydroxyethyl starch. The method comprises the following steps: 1, mixing an aqueous solution of medium molecular weight hydroxyethyl starch obtained in an ultrafiltration step of a medium molecular weight hydroxyethyl starch preparation technology with C1-C3 monohydric alcohol to precipitate the medium molecular weight hydroxyethyl starch from the aqueous solution; and 2, separating the medium molecular weight hydroxyethyl starch precipitated in step 1 to obtain purified medium molecular weight hydroxyethyl starch. The invention also provides the medium molecular weight hydroxyethyl starch obtained through the method. The method has industrialized values and can improve the internal quality of a product. The method has the advantages of reduction of the bacterial endotoxin and glycol level of the finished product, economy, energy saving, environmental protection and energy consumption reduction, and is suitable for real needs of the industrialized mass production.

The invention discloses a composition used for weight control. The composition comprises prebiotics, bamboo shoot fibers, wheat fibers, tea polyphenols, blueberries, resveratrol and probiotic bacteria, wherein the water activity of the prebiotics is less than or equal to 0.1; and the probiotic bacteria comprise bifidobacterium lactis B420 and bifidobacterium breve B-3. The composition disclosed bythe invention contains the bifidobacterium lactis B420 and the bifidobacterium breve B-3 which have the effect of losing weight; meanwhile, dietary fibers capable of obviously increasing the satietyof an eater and raw materials acting on intestinal microecology are also matched; and thus, the composition can promote the quantity of beneficial bacteria in a human body, can reduce the food intakeof the human body and decrease intestinal inflammation, reaches the effect of losing weight by playing a role in multiple aspects, and is effective for a majority of people.

The invention relates to a nanofiber space structure-based controllable bionic tissue engineering scaffold and a preparation method. Firstly, a gelatin-based aqueous solution is mixed with a protein aqueous solution and freeze drying is performed after the pH value of the mixed solution is regulated to be 5-7 to obtain a composite protein porous scaffold, then a culture bacteria solution is dropwise added onto the composite protein porous scaffold; until the composite protein porous scaffold adsorbs the culture bacterium solution to be saturated, a fermentation culture solution is added for static culture, and finally a compound obtained through static culture is immersed into a triton aqueous solution with the concentration of 0.1-5wt% for purification and then freeze drying is performed to obtain the bionic tissue engineering scaffold. The prepared bionic tissue engineering scaffold is of a 3D porous structure with pores communicating with each other; and the average pore diameter is 10-300 [mu]m, and the pore diameter range is adjustable. The method according to the invention can control the density distribution of nanofibers in a three-dimensional space, and the prepared bionic tissue engineering scaffold has a good mechanical property, biocompatibility, low endotoxin and good cell adsorption and proliferation promotion capabilities.

The invention discloses a fusion protein of recombinant clostridium histolyticum type I collagenase. The fusion protein comprises the clostridium histolyticum type I collagenase and tag protein connected to the N end of the clostridium histolyticum type I collagenase. The tag protein comprises a calmodulin binding peptide (CBP) and a streptococcus avidin binding peptide (SBP) which are connected in series. The invention further provides a preparation method and application of the fusion protein of the recombinant clostridium histolyticum I type collagenase. The CBP-SBP is used as a fusion tag, soluble expression of clostridium histolyticum I type collagenase (ColG) can be efficiently promoted, inclusion body renaturation is avoided, purification is facilitated, the production cost is reduced, and the requirements of industrial production can be met.

The invention belongs to the field of assisted reproduction, and in particular relates to a specific adsorption membrane for endotoxin. The endotoxin-specific adsorption membrane of the present invention is specifically as follows: a PVDF hollow fiber membrane is used as a carrier, polymyxin B is used as an adsorbent, wherein polymyxin B is fixed on the carrier in a coupled manner, and the PVDF hollow fiber membrane is HEC is coupled, and a hexamethylenediamine spacer is grafted, and the polymyxin B is coupled to the hexamethylenediamine spacer. It can be used to remove endotoxins in various buffer solutions, culture solutions, operating solutions, etc. involved in the field of assisted reproduction, and effectively reduce the content of endotoxins to an ideal range without affecting the content of active ingredients. The present invention also provides an operation method when the above-mentioned membrane is used in sperm micromanipulation fluid to remove endotoxin. The endotoxin-specific adsorption membrane of the present invention is used for removing endotoxin from the culture solution in the field of assisted reproduction, which is helpful for assisted reproduction to improve the embryo development rate, and the embryo has better morphology.

The invention relates to a method for purifying an escherichia coli expressed viroid particle. The purification method comprises a precipitation step, a first chromatography step, a second chromatography step, a concentration step and a third chromatography step, wherein the precipitation step comprises an ammonium sulfate precipitation step; the first chromatography step comprises a molecular screen chromatography step; the second chromatography step comprises a hydroxyapatite chromatographic analysis; the concentration step comprises an ultrafiltration and concentration step; the third chromatography step comprises a heparin affinity chromatography step.

The invention discloses a swine straw feed and its preparation method. The swine straw feed is mainly prepared by mixing raw materials of corn straw, wheat straw 30-40 and Chinese herbal medicines. The formula provided by the invention has an advantage of reasonable collocation of proportions of proteins in corn straw, wheat straw, corn and the like, can basically satisfy the growth need of pigs, and can be used to increase income of the vast majority of peasants and improve the quality of pork. As the formula contains traditional Chinese medicines of cortex acanthopanacis, Chinese chive seed and the like, the formula has an effect of supplementing the center and boosting qi and can be used to increase disease resistance of pigs. By the adoption of the formula, pigs have bright furs and a good state during the feeding process. Pteridium aquilinum and Gynura Bicolor both belongs to edible wild herb, have an effect of heat clearing and detoxifying, and can minimize endotoxin in pigs and raise immunity and disease resistance. Galagal rhizome and haw have a function of strengthening stomach and can guarantee a good appetite for pigs. Peach leaves have laxation and insecticidal effects and can be used to prevent pigs from being infected with diseases. The formula provided by the invention is reasonable and scientific.

The invention provides eye drops and a preparation method thereof, and relates to the eye drops. The invention provides the eye drops which have the advantages of shorter action time, lower cost, better effect and capacity of resisting corneal neovascularization and quickly repairing epithelium compared with the conventional eye drops, and a preparation method thereof. The eye drops comprise the following components in percentage by volume: 0.1 to 5 percent of bovine serum, 5 to 15 percent of thickener, 1 to 5 percent of pH regulation solution, 0.1 to 2 percent of osmotic pressure buffer, 0.5 to 2 percent of antibiotic, 5 to 30 percent of original solution of kallikrein-binding protein (SA3K) and the balance of balanced salt solution. According to a prescription of the eye drops, the preparation method comprises the following steps of: adding the thickener, the bovine serum, the antibiotic and the original solution of SA3K into the balanced salt solution; uniformly mixing; regulating the pH value to be 7.2 to 7.4 by using the pH regulator; regulating the osmotic pressure to be 350 to 380mOsm / L by using the osmotic pressure buffer; and filtering and degerming by using a membrane to obtain the SA3K eye drops.

The invention discloses and provides a recombinant histolytic clostridium type II collagenase. The recombinant histolytic clostridium type II collagenase comprises a histolytic clostridium type II collagenase and a tag protein connected to an N end of the histolytic clostridium type II collagenase, the tag protein comprises fasciola hepatica calcium binding protein (Fh8); preferably, the amino acid sequence of the fasciola hepatica calcium binding protein is SEQ ID NO: 2. The invention also provides an expression vector and recombinant engineering bacteria for expressing the recombinant clostridium histolyticum type II collagenase, and also provides a preparation method of the clostridium histolyticum type II collagenase. The recombinant clostridium histolyticum type II collagenase can be efficiently expressed, does not form inclusion bodies, is easy to separate and purify, and is beneficial to industrial production of the clostridium histolyticum type II collagenase.

The invention relates to the technical field of sludge treatment, in particular to a harmless treatment method for sludge in an urban sewage treatment plant. The invention provides a sludge harmless treatment method which comprises the following steps of: firstly, fully mixing water-containing sludge with magnesite powder, adding potassium monopersulfate composite salt, fully aerating the sludge, introducing the sludge into a reactor with a UV-C ultraviolet lamp for continuous digestion, and finally dehydrating and drying the obtained clean sludge. The sludge harmless treatment method is simple in process, controllable in cost and thorough in harmless treatment, development and application of finally obtained clean sludge powder are facilitated, and the obtained sludge powder can be used for manufacturing cement, bricks, tiles, fertilizers and the like.

The invention discloses pig feed capable of improving disease resistance of pigs. The pig feed capable of improving the disease resistance of pigs is characterized by comprising, by weight, 50-60 parts of bran, 5-10 parts of rice bran, 12-18 parts of bean cakes, 3.2-3.8 parts of fish meal, 0.8-1.2 parts of bone meal, 3-8 parts of cushaw stems, 0.10-0.20 part of lysine, 0.10-0.20 part of glutamic acid, 0.3-0.5 part of salt, 1-3 parts of garlic, 1-3 parts of wrinkled giant hyssop, 3-5 parts of red dates, 0.5-1.0 part of polygala tenuifolia, 0.5-0.8 part of endothelium corneum gigeriae galli, 3-5 parts of dandelion, 0.05-0.15 part of multivitamin, 0.4-0.6 part of gunk and 3-5 parts of distillers' grains. The pig feed contains the agastache rugosus which can invigorate stomach and promote appetite of the pigs. The red dates and the polygala tenuifolia can soothe the nerves, enable the pigs to be settled, reduce activity of the pigs and physical output, and fatten as soon as possible. The garlic and the dandelion can invigorate stomach, sterilize, detoxify, improve immunity of the pigs, and reduce diseases and enotoxin of the pigs, and therefore the pig feed can improve meat quality of the pigs, and is environmental-friendly and safe.

The invention relates to a composite bacterial cellulose dressing with a controllable nanofiber spatial structure and a preparation method. First, a gelatin-based aqueous solution is added dropwise to a non-woven material containing protein components, and after drying, cultured bacteria are added dropwise onto it. After the composite non-woven material adsorbs and cultivates the bacterial liquid and is saturated, adding the fermentation medium for static culture, and finally immersing the composite obtained from static culture in a triton aqueous solution with a concentration of 0.1-1 wt% for purification and drying. A non-woven material composite bacterial cellulose dressing is obtained; the prepared dressing has a sparse and dense structure, specifically, nanofibers of bacterial cellulose are distributed on the non-woven micron fibers, and the distribution and density of the nanofibers are adjustable. The non-woven material composite bacterial cellulose dressing prepared by the invention has good biocompatibility, mechanical properties and wound exudate absorption performance, and has huge applications in the biomedical fields such as wound dressings, human body repair materials and tissue engineering materials. prospect.

The invention discloses a bovine pasteurella multocida inactivated vaccine and a preparation method thereof, and belongs to the technical field of veterinary biological products. The vaccine comprisesantigens and adjuvants, wherein the antigens are a bovine pasteurella multocida capsule type B C45-2 strain and a C47-2 strain. The preparation method comprises the following steps: performing inoculated culture on strains, performing shake culture, streaking a culture on an agar plate for culture, selecting bacterial colonies, inoculating a fresh blood slant with the selected bacterial colonies,performing culturing to obtain primary seeds, inoculating a culture medium with the primary seeds, and performing shake culture to obtain secondary seeds; mixing the secondary seeds, and performing inoculated culture to obtain zymophyte liquid, wherein the culture process is optimized, so that the antigen quantity is multiplied; and performing centrifuging to remove endotoxin, transferring bacterial sludge into normal saline, adding formaldehyde, performing inactivating, adding aluminum hydroxide glue, thiomersalate and phenol, performing full oscillating, and performing sub-packaging. The dosage of the bovine pasteurella multocida inactivated vaccine for immunizing a calf is 2-3mL and is less than the existing injection dosage, the side effect is greatly reduced, and the bovine pasteurella multocida inactivated vaccine has popularization and application values in the fields of bovine pasteurella multocida inactivated vaccines and even all bacterial culture processes.

The invention belongs to the field of skin medicine, and particularly relates to a hypoallergenic and anti-aging yak collagen compound. The invention provides the yak collagen with low endotoxin and low sensitization, and the yak collagen is low in cytotoxicity, can promote cell proliferation and cell adhesion, and has good properties such as oxidation resistance and aging resistance. Meanwhile, the yak collagen composition containing yak collagen, hyaluronic acid, dipotassium glycyrrhizinate, acetyl hexapeptide-8, ellagic acid, a Salix alba bark extract, nicotinamide, vitamin E and resveratrol is obtained through screening, and the yak collagen composition has low sensitization and more excellent antioxidant and anti-aging performance, and can be widely applied to the field of cosmetics or dermatology.