Blastocyst culture medium and preparation method thereof

A technology of culture solution and blastocyst, which is applied in the field of blastocyst culture solution and its preparation, can solve the problems of high cost, poor performance of solution effectiveness, etc., achieve strict detection, inhibit the growth of bacteria, and avoid The effect of unknown factors

Active Publication Date: 2017-10-03
山东威高瑞生医疗器械有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the blastocyst culture medium in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorated and continues to be used, it will cause irreversible damage to the experiment or clinical operation. the result of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Blastocyst culture medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The present invention will be further described below by way of examples.

[0030] 1. Formula and preparation method of blastocyst culture medium

[0031] The formula of table 1 blastocyst culture medium

[0032]

[0033] Note: The phenol red in the formula is "0", which means that the blastocyst culture medium does not contain phenol red; the human serum albumin is "0", which means that human serum albumin is not added before subpackaging, but added when using.

[0034] The preparation method of blastocyst culture fluid of the present invention comprises the following steps:

[0035] (1) Preparation of base fluid

[0036] a. First weigh each component according to the proportion and place them separately;

[0037] b. Dissolve the weighed components (except for bactericide, selective indicator, sodium bicarbonate and human serum albumin) in ultrapure water. 3 the principle of last access;

[0038] c, add weighed bactericide, indicator and sodium bicarbonate sele...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a blastocyst nutrient solution and a preparation method thereof. The blastocyst nutrient solution comprises a composition for treating and culturing a zygote, a bactericide, a selectively added indicator and a selectively added human serum albumin. Each index of the blastocyst nutrient solution reaches the standard of an in-vitro nutrient solution, the use is safe, and whether the nutrient solution is deteriorated or not can be easily determined, and therefore a powerful guarantee is provided to the assisted reproductive technology.

Description

technical field [0001] The invention relates to artificial assisted reproductive technology, in particular to a blastocyst culture solution and a preparation method thereof. Background technique [0002] Blastocyst culture fluid has been widely used in the development and culture of embryos in test-tube baby technology. The purpose of the blastocyst culture medium is: first, to provide nutrients for the survival and development of the embryo; second, to provide the necessary environment for the survival of the embryo. The use of blastocyst culture medium: In the laboratory or clinic, in the carbon dioxide environment, it provides nutrients and necessary environment for the division and development of blastocysts in vitro. [0003] At present, the blastocyst culture medium in my country's assisted reproductive technology relies on imported finished products, the cost is high, and the effectiveness of the solution cannot be well expressed. If the culture medium is deteriorate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/073
Inventor 王维华吕汝举刘美静庄巍王英超
Owner 山东威高瑞生医疗器械有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap