Composition for oocyte and skin adult stem cell culture and applications thereof

A technology for adult stem cells and oocytes, which is applied in the field of oocytes and skin adult stem cell culture compositions, and can solve problems such as difficult application

Active Publication Date: 2015-08-12
LANNUO BIOTECH WUXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The above applications all involve the in vitro expansion and culture of skin adult stem cells. However, skin adult stem cells are easily differentiated into fibroblasts in vitro, making the application of autologous skin adult stem cells and artificial skin constructed from autologous skin adult stem cells clinically difficult. Realize, the application of above-mentioned composition can effectively solve this problem

Method used

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  • Composition for oocyte and skin adult stem cell culture and applications thereof
  • Composition for oocyte and skin adult stem cell culture and applications thereof
  • Composition for oocyte and skin adult stem cell culture and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] This example provides a composition for culturing oocytes and skin stem cells, labeled LN01, which is composed of cysteamine and LIF, and the ratio of cysteamine to LIF is 100mmol:10 3 IU.

[0073] This example provides a composition for culturing oocytes and skin stem cells, labeled LN011, which is composed of cysteamine and LIF, and the ratio of cysteamine to LIF is 1 mmol: 1000 IU.

[0074] This example provides a composition for culturing oocytes and skin stem cells, labeled LN012, which is composed of cysteamine and LIF, and the ratio of cysteamine and LIF is 50.5 mmol: 505 IU.

[0075]This embodiment provides a composition for culturing oocytes and skin stem cells, labeled LN014, which is composed of cysteamine and LIF, and the ratio of cysteamine and LIF is 100mmol: 10IU.

Embodiment 2

[0077] This example provides a composition for culturing oocytes and skin stem cells, labeled LN02, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 100mmol: 10 3 IU: 10mmol.

[0078] This example provides a composition for culturing oocytes and skin stem cells, labeled LN021, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 1 mmol: 10 IU: 10 mmol .

[0079] This example provides a composition for oocyte and skin stem cell culture, labeled LN022, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 50.5mmol: 100 IU : 0.1 mmol.

[0080] This example provides a composition for oocyte and skin stem cell culture, labeled LN023, which is composed of cysteamine, LIF and Y27632, and the ratio of cysteamine, LIF and Y27632 is 100mmol: 505IU: 5mmol .

[0081] This example provides a composition for culturing oocytes and skin stem cells, labeled L...

Embodiment 3

[0084] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos. The cystamine concentration was 50 mM, the LIF concentration was 500 IU / mL, and the Y27632 concentration was 5 mM.

[0085] This example provides a culture medium for the culture of goat oocytes, IVF fertilized eggs or IVF embryos. The cystamine concentration was 100 mM, the LIF concentration was 10 3 IU / mL, Y27632 concentration is 10 mM.

[0086] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos, labeled as LNCSG03, added with a composition composed of cysteamine, leukemia inhibitory factor and Rho kinase inhibitor, half The cystamine concentration was 200 mM and the LIF concentration was 2 × 10 3 IU / mL, Y27632 concentration is 20mM.

[0087] This example provides a culture solution for the culture of goat oocytes, IVF fertilized eggs or IVF embryos, labeled as LNCSG04, added with a composition com...

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Abstract

The invention provides a composition for oocyte and skin adult stem cell culture and applications thereof. The composition can improve the in-vitro maturation rate of oocyte, cleavage rate of ovum, and rate that embryo grows into mulberry and blastocyst; and thus the growth from in-vitro fertilized ovum to embryo is greatly promoted. At the same time, the differentiation from skin adult stem cells to fibroblast is effectively prohibited by the composition, or the self renewing and regeneration of skin adult stem cells are maintained. The composition comprises cysteamine, leukemia inhibitory factor, and Rho kinase inhibitor Y27632.

Description

technical field [0001] The invention relates to a composition and application for culturing oocytes and skin adult stem cells. Background technique [0002] Oocytes are produced on the mother's ovary. Follicles on the ovary include oocytes and follicular cells. During the growth of the follicle, the primary oocyte (primary oocyte) stops in the prophase of the first mature division of meiosis. When puberty begins, under the action of follicle stimulating hormone (FSH), the progenitor primary oocyte The cell volume increases and forms a zona pellucida around the periphery of the oocyte. The follicle cells grow into multiple layers of cells, surround the oocyte, and form a follicle cavity in the follicle, so it is called a secondary follicle. . In the mature follicle, the primary oocyte completes the first meiosis under the action of the hormone LH and other factors, forms a secondary oocyte, or a mature oocyte, and discharges the first polar body, polar body The body is lo...

Claims

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Application Information

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IPC IPC(8): C12N5/075C12N5/074A61K45/06A61P17/02A61K8/98
Inventor 杜福良杨澜安礼友薛非
Owner LANNUO BIOTECH WUXI
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