36 results about "Single nucleotide polymorphism analysis" patented technology

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

The invention relates to an MLCR probe, a two-step reaction mode and a suspension chip detection capture probe. The MLCR probe is one group of four single-chain DNA probes synthesized aiming at each point to be tested, the tail end of the MLCR probe is connected with a general sequence and used for converting LCR into MLCR; in the two-step reaction mode, the MLCR probe firstly carries out MLCR reaction to be converted into a connecting product, general sequences at the tail end of the connecting product are respectively matched with a general primer, and then PCR reaction for equally amplifying the connecting product is carried out; and the capture probe spans incision recognized by a heat-resisting lignase in the point to be tested, and can be hybridized with a product obtained by the second step PRC amplification at a chip inspection temperature. The invention overcomes the defects of low detection sensitivity of LCR and MLPA and difficult amplification of multiple PCRs by combining with the advantages of the LCR and the MLPA, has the characteristics of high flux, super sensitivity and high specificity, and is suitable for gene testing and Single nucleotide polymorphism analysis of various samples, especially traces and degraded DNA samples.

The invention relates to a method for auxiliarily determining warfarin dosage for Chinese Han patients by utilizing single nucleotide polymorphism analysis and a biological chip. In particular, the invention provides a method for acquiring grades useful in determining the warfarin dosage for the Chinese Han patients, which comprises the following steps: (1) acquiring a nucleic acid sample from a patient; (2) detecting the nucleic acid sample aiming at one or more single nucleotide polymorphism sites selected from the following groups: rs9934438 site of VKORC1 gene, rs1057910 site and rs9332127 site of CYP2C9 gene, and rs4653436 site of EPHX1 gene; (3) determining genotypes of the single nucleotide polymorphism sites; and (4) grading the genotypes of the single nucleotide polymorphism sites, wherein the grades obtained in step (4) are helpful in determining the warfarin dosage for the patient. The invention also provides the biological chip used for the method.

A system for detecting the ligase reaction of nucleic acid and the ligase chain reaction of nucleic acid is compose dof the nucleic acid probes of molecular label, amplified nucleic fragrance, Taq ligase and buffer solution. Its real-time analysis method includes such steps as adding molecular label, Taq ligase, amplifying primer, and single-chain or dual-chain target nucleic acid to the buffer solution, LDR or LCR temp cycle, and detecting the fluorescent intensity of specimen in low-temp cycle.

Described herein are assays, kits and methods for treating mood disorders by testing for one or more polymorphisms in a specific group of genes and for analyzing the results of polymorphism testing; the genes included may converge in one or more signaling pathways, and may be epigenetic. The genes are included based on the relationships of the proteins encoded by the genes in the context of particular signaling pathways and provide a diagnostically relevant nexus. Also described herein are methods of presenting the data collected by the screen, including methods of delivering interpretive comments and / or treatment guidance based on the results of the genetic screening either individually or based on the genetic composition of particular clusters of genes which may be related to each other. Importantly, drugs which modulate these genetic disturbances are described for targeted therapeutic use based upon companion diagnostic method.

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

The invention provides a method for denatured bubble mediated target nucleic acid amplification and a related kit and use thereof. According to the method, generation of denatured bubbles in double-stranded target nucleic acid molecules is promoted through rapid temperature-changing thermal circulation, so that the strand displacement amplification (SEA) reaction is accelerated. The kit comprises a specially designed primer and a polymerase for performing the method. The method and kit can be used in various situations, such as diagnosis of infectious or genetic diseases, sample quality control, and single nucleotide polymorphism (SNP) analysis.

The present invention relates to a dumbbell-structure oligonucleotide (DSO), a nucleic acid amplification primer comprising the same, and a nucleic acid amplification method using the same and, more specifically, to a method for multiple gene amplification and single-nucleotide polymorphism analysis using a dumbbell-structure oligonucleotide capable of excluding non-specific amplification products prior to binding to a template in a first cycle in performing a polymerase chain reaction. The present invention suppresses undesired amplification products at room temperature using a dumbbell structure oligonucleotide (DSO) generated by adding any nucleotide sequence designed to allow the 5'-terminal oligonucleotide and the 3'-terminal oligonucleotide to complimentarily bind to each other, a 3'-terminal template dependent specific nucleotide sequence, and a universal nucleotide pair for linking the two nucleotide sequences, prior to binding to a template in every first cycle at the time of the polymerase chain reaction (PCR), and as a result, efficiently increases sensitivity and specificity through the reduction in non-specific amplification products, thereby achieving the innovation of the gene amplification method.

The invention provides an asymmetric cyanine dye compound (I) and application of the asymmetric cyanine dye compound serving as a quenching agent. The asymmetric cyanine dye fluorescence quenching agent compound can effectively quench fluorescence of an ROX channel, meanwhile, double DNA strands can be stabilized, the melting temperature in the PCR process is increased by 2 DEG C to 3 DEG C, the compound not only can be applied to a conventional real-time fluorescence quantitative PCR detection method but also can be effectively used in the field of analysis for single nucleotide polymorphism. Please see the formula of the asymmetric cyanine dye compound in the specification.

A system for detecting the ligase reaction of nucleic acid and the ligase chain reaction of nucleic acid is compose dof the nucleic acid probes of molecular label, amplified nucleic fragrance, Taq ligase and buffer solution. Its real-time analysis method includes such steps as adding molecular label, Taq ligase, amplifying primer, and single-chain or dual-chain target nucleic acid to the buffer solution, LDR or LCR temp cycle, and detecting the fluorescent intensity of specimen in low-temp cycle.

The invention relates to methods of evaluating MS severity based on analysis of single nucleotide polymorphisms (SNPs) and to products and kits for use in such methods. The methods include a method of assessing a multiple sclerosis disease severity phenotype in a human subject having multiple sclerosis, by determining the genotype of the subject at one or more positions of single nucleotide polymorphism (SNP) selected from: rs2107538, rs1137933, rs1318, rs2069763, rs423904, rs876493, rs10243024, rs10259085, rs1042173, rs10492503, rs10492972, rs12047808, rs12202350, rs12861247, rs13353224, rs1350666, rs1555322, rs1611115, rs17641078, rs1805009, rs2028455, rs2032893, rs2049306, rs2066713, rs2074897, rs2076530, rs2187668, rs2213584, rs2227139, rs2234978, rs2239802, rs2395182, rs260461, rs28386840, rs3087456, rs3135388, rs3741981, rs3756450, rs3781202, rs3787283, rs3808585, rs4128767, rs4404254, rs4473631, rs4680534, rs6077690, rs6457594, rs6570426, rs659366, rs6917747, rs7208257, rs7528684, rs7577925, rs762550, rs7956189, rs7995215, rs8049651, rs8702, rs9808753 and rs987107, and / or a SNP in linkage disequilibrium with any one of said SNPs.

The invention discloses a method for selecting and breeding soft-shelled soft-shelled turtles. SNP single nucleotide polymorphism analysis is used to determine Chinese soft-shelled soft-shelled turtle seedlings with excellent properties. Conduct non-specific viral pathogen detection on Chinese soft-shelled turtle seedlings, and screen out Chinese soft-shelled soft-shelled turtle seedlings without specific viruses; disinfect the breeding ponds to make them meet the breeding requirements; prepare multi-bacteria-sensitive bacteriophage microecological preparations; During the breeding process, the multi-bacteria-sensitive bacteriophage microecological preparations are regularly sprinkled; the water quality of the aquaculture water is regularly tested to determine the water quality, and the frequency of sprinkling the multi-bacteria-sensitive bacteriophage microecological preparations is adjusted as needed. The breeding method of the invention is highly operable, universal and economical.

A method for deteting the mutation at gene site by combining allelic specific amplification with nano-gold probe inclues designing the allelic specific primer complementary with mutant gene but not with wild gene, allelic specific amplifying to obtain double-stranded DNA (product A) and residual single-stranded DNA-primer (product B), and sequentially adding said product and electrolyte solution to nano-gold sol. The color of solution is changed from red to blue for the product A or its not changed for the product B.

The invention provides an asymmetric cyanine dye compound (I) and application of the same as a quenching agent. The invention provides the asymmetric cyanine dye fluorescence quenching compound which can effectively quench fluorescence of FAM and stabilize DNA double strands, enables melting temperature in the PCR process to increase 2 to 3 DEG C, is applicable to a conventional real time fluorescence quantification PCR detection method and can be effectively applied to the field of analysis of single nucleotide polymorphism.

The invention provides an asymmetric cyanine dye compound (I) and an application of the cyanine dye compound as a quenching agent. By virtue of the asymmetric cyanine dye fluorescence quenching agent compound provided by the invention, 650nm-channel fluorescence can be effectively quenched and the melting temperature of stable double strands of DNA is increased by 2-3 DEG C in the PCR (Polymerase China Reaction) process. The asymmetric cyanine dye compound can be used for a conventional real-time fluorescent quantitative PCR detection method and can be also effectively used in the field of single nucleotide polymorphism analysis.

The present invention relates to a dumbbell structure oligonucleotide (dumbbell structure oligonucleotide, DSO), a nucleic acid amplification primer comprising the same and a nucleic acid amplification method using the primer, more specifically, to a method for performing polymerase chain reaction , a method for multiple gene amplification and single nucleotide polymorphism analysis using dumbbell structure oligonucleotides that can eliminate non-specific amplification products before combining with templates in each first cycle. In the polymerase chain reaction (polymerase chain reaction, PCR), the present invention utilizes dumbbell structure oligonucleotides (dumbbell structure oligonucleotide, DSO) to suppress unintended amplification products at room temperature, thereby reducing non-specific amplification products, Sensitivity and specificity can be improved efficiently, and the method of gene amplification can be improved. The dumbbell structure oligonucleotide can be obtained by attaching an arbitrary base sequence and a 3'-terminal template-dependent specific base sequence and connecting the two base sequences. , and the 5'-terminal oligonucleotide and the 3'-terminal oligonucleotide of the dumbbell structure oligonucleotide are designed to be in each first cycle before binding to the template Complementary binding.