36 results about "Single nucleotide polymorphism analysis" patented technology

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.

The invention provides an asymmetric cyanine dye compound (I) and application of the asymmetric cyanine dye compound serving as a quenching agent. The asymmetric cyanine dye fluorescence quenching agent compound can effectively quench fluorescence of an ROX channel, meanwhile, double DNA strands can be stabilized, the melting temperature in the PCR process is increased by 2 DEG C to 3 DEG C, the compound not only can be applied to a conventional real-time fluorescence quantitative PCR detection method but also can be effectively used in the field of analysis for single nucleotide polymorphism. Please see the formula of the asymmetric cyanine dye compound in the specification.

A system for detecting the ligase reaction of nucleic acid and the ligase chain reaction of nucleic acid is compose dof the nucleic acid probes of molecular label, amplified nucleic fragrance, Taq ligase and buffer solution. Its real-time analysis method includes such steps as adding molecular label, Taq ligase, amplifying primer, and single-chain or dual-chain target nucleic acid to the buffer solution, LDR or LCR temp cycle, and detecting the fluorescent intensity of specimen in low-temp cycle.

The invention relates to methods of evaluating MS severity based on analysis of single nucleotide polymorphisms (SNPs) and to products and kits for use in such methods. The methods include a method of assessing a multiple sclerosis disease severity phenotype in a human subject having multiple sclerosis, by determining the genotype of the subject at one or more positions of single nucleotide polymorphism (SNP) selected from: rs2107538, rs1137933, rs1318, rs2069763, rs423904, rs876493, rs10243024, rs10259085, rs1042173, rs10492503, rs10492972, rs12047808, rs12202350, rs12861247, rs13353224, rs1350666, rs1555322, rs1611115, rs17641078, rs1805009, rs2028455, rs2032893, rs2049306, rs2066713, rs2074897, rs2076530, rs2187668, rs2213584, rs2227139, rs2234978, rs2239802, rs2395182, rs260461, rs28386840, rs3087456, rs3135388, rs3741981, rs3756450, rs3781202, rs3787283, rs3808585, rs4128767, rs4404254, rs4473631, rs4680534, rs6077690, rs6457594, rs6570426, rs659366, rs6917747, rs7208257, rs7528684, rs7577925, rs762550, rs7956189, rs7995215, rs8049651, rs8702, rs9808753 and rs987107, and / or a SNP in linkage disequilibrium with any one of said SNPs.

The invention discloses a method for selecting and breeding soft-shelled soft-shelled turtles. SNP single nucleotide polymorphism analysis is used to determine Chinese soft-shelled soft-shelled turtle seedlings with excellent properties. Conduct non-specific viral pathogen detection on Chinese soft-shelled turtle seedlings, and screen out Chinese soft-shelled soft-shelled turtle seedlings without specific viruses; disinfect the breeding ponds to make them meet the breeding requirements; prepare multi-bacteria-sensitive bacteriophage microecological preparations; During the breeding process, the multi-bacteria-sensitive bacteriophage microecological preparations are regularly sprinkled; the water quality of the aquaculture water is regularly tested to determine the water quality, and the frequency of sprinkling the multi-bacteria-sensitive bacteriophage microecological preparations is adjusted as needed. The breeding method of the invention is highly operable, universal and economical.

A method for deteting the mutation at gene site by combining allelic specific amplification with nano-gold probe inclues designing the allelic specific primer complementary with mutant gene but not with wild gene, allelic specific amplifying to obtain double-stranded DNA (product A) and residual single-stranded DNA-primer (product B), and sequentially adding said product and electrolyte solution to nano-gold sol. The color of solution is changed from red to blue for the product A or its not changed for the product B.

The invention provides an asymmetric cyanine dye compound (I) and application of the same as a quenching agent. The invention provides the asymmetric cyanine dye fluorescence quenching compound which can effectively quench fluorescence of FAM and stabilize DNA double strands, enables melting temperature in the PCR process to increase 2 to 3 DEG C, is applicable to a conventional real time fluorescence quantification PCR detection method and can be effectively applied to the field of analysis of single nucleotide polymorphism.

The present invention relates to a dumbbell structure oligonucleotide (dumbbell structure oligonucleotide, DSO), a nucleic acid amplification primer comprising the same and a nucleic acid amplification method using the primer, more specifically, to a method for performing polymerase chain reaction , a method for multiple gene amplification and single nucleotide polymorphism analysis using dumbbell structure oligonucleotides that can eliminate non-specific amplification products before combining with templates in each first cycle. In the polymerase chain reaction (polymerase chain reaction, PCR), the present invention utilizes dumbbell structure oligonucleotides (dumbbell structure oligonucleotide, DSO) to suppress unintended amplification products at room temperature, thereby reducing non-specific amplification products, Sensitivity and specificity can be improved efficiently, and the method of gene amplification can be improved. The dumbbell structure oligonucleotide can be obtained by attaching an arbitrary base sequence and a 3'-terminal template-dependent specific base sequence and connecting the two base sequences. , and the 5'-terminal oligonucleotide and the 3'-terminal oligonucleotide of the dumbbell structure oligonucleotide are designed to be in each first cycle before binding to the template Complementary binding.