4 results about "Single nucleotide polymorphism analysis" patented technology

The invention discloses a primer composition and application of the primer composition in detection of animal bifidobacterium subsp. Lactis HN019. According to the present invention, bifidobacterium animalis subsp. Lactis HN019 is adopted as an object, bacterial strain specific genetic markers are screened through whole genome sequencing and single nucleotide polymorphism analysis, and a fluorescent quantitative PCR technology is combined so as to obtain the efficient, specific and stable HN019 quantitative detection primer composition and the method thereof; through system verification, the method is suitable for an HN019 strain level detection technical framework in scientific research and industrial application scenes, and also provides key technical support for market supervision and industrial healthy development of probiotic foods.

This article provides a method and related kits for denaturing vesicle-mediated target nucleic acid amplification, along with their applications. This method accelerates strand displacement amplification (SEA) reactions by promoting the generation of denaturing vesicles in double-stranded target nucleic acid molecules through rapid temperature-changing thermal cycling. The kits contain specially designed primers and polymerases for performing this method. The methods and kits disclosed herein can be used in various situations, such as the diagnosis of infectious or genetic diseases, sample quality control, and single nucleotide polymorphism (SNP) analysis.

The present invention relates to a DNA polymerase variant belonging to Family A and its use, and to a DNA polymerase variant that smoothly polymerizes when the base at the 3' end of a primer is complementary to a template, and polymerization is inhibited when the base at the 3' end of a primer is not complementary to a template, thereby easily discriminating between the two cases, and to a PCR method using the variant and a PCR kit containing the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping) and somatic mutation detection, etc.

The present invention relates to a Microparticle probe for single nucleotide polymorphisms. The present invention provides a Microparticle probe for single nucleotide polymorphisms comprising a microparticle; capture probes that are introduced to the surface of the microparticle and contain a complementary sequence to a target nucleic acid; and reporter nucleic acids that are introduced to the surface of the microparticle and generate a signal by an external stimulus.