2 results about "Single nucleotide polymorphism analysis" patented technology

This article provides a method and related kits for denaturing vesicle-mediated target nucleic acid amplification, along with their applications. This method accelerates strand displacement amplification (SEA) reactions by promoting the generation of denaturing vesicles in double-stranded target nucleic acid molecules through rapid temperature-changing thermal cycling. The kits contain specially designed primers and polymerases for performing this method. The methods and kits disclosed herein can be used in various situations, such as the diagnosis of infectious or genetic diseases, sample quality control, and single nucleotide polymorphism (SNP) analysis.

The present invention relates to a DNA polymerase variant belonging to Family A and its use, and to a DNA polymerase variant that smoothly polymerizes when the base at the 3' end of a primer is complementary to a template, and polymerization is inhibited when the base at the 3' end of a primer is not complementary to a template, thereby easily discriminating between the two cases, and to a PCR method using the variant and a PCR kit containing the variant. The present invention is useful for single nucleotide polymorphism analysis (SNP genotyping) and somatic mutation detection, etc.