Unlock instant, AI-driven research and patent intelligence for your innovation.
Asymmetric cyanine dye compound and its application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of cyanine dyes and compounds, applied in the field of application of ROX channel fluorescence quenchers
Inactive Publication Date: 2015-12-30
ZHEJIANG UNIV
View PDF4 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] In the prior art, ROX fluorescent dyes are generally used to adjust the difference between PCR tubes and tubes caused by PCR loading errors. At present, there is no ROX channel quencher that is widely used.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0033] Embodiment 1: the synthesis of compound (III)
[0034]
[0035] 2,3-Dimethylbenzoxazole p-toluenesulfonate (180mg, 0.52mmol), benzamidine (160mg, 0.8mmol) and 2ml of acetic anhydride were refluxed for 30 minutes, cooled and dropped into ether to obtain Product 170mg.
[0037] Embodiment 2: the synthesis of compound (II)
[0038]
[0039] 4-Methylquinoline (4.8ml, 0.0363mol) and methyl 2-(4-(bromomethyl)-2-nitrophenyl)propionate (prepared according to NaokiYamakawa et al., Bioorganic & Medicinal Chemistry 19(2011) 3299-3311) (16.44g, 0.05445mol) mixed, react overnight at 110°C, after cooling, add 20ml of methanol to dissolve, then hang and steam to obtain a viscous solid, then add 200ml of acetone and stir, after stirring for 2h, the product precipitates, filter, and dry to obtain the product Compound (II) 6.4g.
[0041] Embodiment 3: the synthesis of compound (I-1)
[0042]
[0043] Compound III (58mg, 0.13mmol) and compound (II) (89mg, 0.2mmol) were dissolved in 2ml of pyridine, and heated to reflux for 30 minutes. After the reaction was completed, the pyridine was removed by rotary evaporation, and the residue was poured into water, and 5% Hydrochloric acid, filter after the product is precipitated, dissolve the product obtained by filtration in 30ml of methanol and water (volume ratio 1:1), add 80mg of sodiumhydroxide for hydrolysis, after hydrolysis, drop concentrated hydrochloric acid again, after the product precipitates, Filter and dry to get the final product.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention provides an asymmetric cyanine dye compound (I) and application of the asymmetric cyanine dye compound serving as a quenching agent. The asymmetric cyanine dye fluorescencequenching agent compound can effectively quench fluorescence of an ROX channel, meanwhile, double DNA strands can be stabilized, the melting temperature in the PCR process is increased by 2 DEG C to 3 DEG C, the compound not only can be applied to a conventional real-time fluorescence quantitative PCR detection method but also can be effectively used in the field of analysis for single nucleotide polymorphism. Please see the formula of the asymmetric cyanine dye compound in the specification.
Description
(1) Technical field [0001] The invention relates to an asymmetric cyanine dye compound and its application as a fluorescence quencher, especially a ROX channel fluorescence quencher. (2) Background technology [0002] Nucleic acid hybridization technology is one of the basic techniques of molecular biology, which can be used for diagnosis of genetic diseases, human body identification, microbial identification, paternity identification, and is gradually being used for specific, sensitive and rapid diagnosis of viruses in recent years. [0003] An important aspect of nucleic acid hybridization techniques is the detection of hybridization. A frequently used tool is the fluorescent oligonucleotide probe. A particularly useful type of fluorescent probe is the self-quenching probe, which includes a reporter The dye and the quencher dye interact through the process of fluorescence resonanceenergy transfer (FRET). Although the design of different probes using this motif may diffe...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
