Mismatched linear double-stranded oligonucleotide probe and detection method of lncRNA

A technology of oligonucleotide probe and detection method, which is applied in the detection field of linear double-stranded oligonucleotide probe and lncRNA, to achieve the effect of simplified probe design, simple probe design and low background signal

Pending Publication Date: 2022-01-21
SHANDONG NORMAL UNIV
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  • Claims
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Problems solved by technology

However, this approach requires complex nanoparticle synthesis and modification, carefully designed imaging probes, or the assistance of foreign metal ions.

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  • Mismatched linear double-stranded oligonucleotide probe and detection method of lncRNA
  • Mismatched linear double-stranded oligonucleotide probe and detection method of lncRNA
  • Mismatched linear double-stranded oligonucleotide probe and detection method of lncRNA

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Embodiment Construction

[0032] It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0033] It should be noted that the terminology used here is only for describing specific embodiments, and is not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0034] As introduced in the background technology, the lncRNA detection methods reported in the prior art have problems such as cumberso...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a mismatched linear double-stranded oligonucleotide probe and a detection method of lncRNA. The mismatched linear double-stranded oligonucleotide probe is adopted to directly detect long-chain non-coding RNA (lncRNA) in vitro or in living cells, any enzyme amplification is not needed, uncombined probes do not need to be separated, the strand displacement reaction rate of the target lncRNA can be increased by introducing mismatched basic groups into the oligonucleotide probe, and the advantages of being low in background signal and high in detection sensitivity are achieved. The mismatched oligonucleotide probe can detect the expression level of lncRNA in cells in various cell lines, cancer cells are distinguished from normal cells, and a useful platform is provided for rapidly and easily detecting low-abundance nucleic acid in living cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mismatched linear double-stranded oligonucleotide probe and a detection method for lncRNA. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Fluorescent probes consist of two basic components: a recognition component and a conversion component. Fluorescent probes can convert various signaling events, such as intracellular DNA / RNA concentration and enzyme activity, into measurable fluorescence values. Fluorescent probes have been used to visualize cellular components directly in living cells without damage, and the fluorescent signal allows high-fidelity recording of t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6823C12Q1/6886C12N15/11
CPCC12Q1/6823C12Q1/6886C12Q2600/158C12Q2600/178C12Q2565/519C12Q2537/1373C12Q2563/107C12Q2565/601
Inventor 张春阳张艳杜雪珂苏献伟邹笑然
Owner SHANDONG NORMAL UNIV
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