Complexes used for detecting the reaction for cleaning free radical with anti-oxidants and detection method there of

An antioxidant and free radical technology, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve problems such as difficult purification and preservation of samples, harsh synthesis conditions, and difficult synthesis

Inactive Publication Date: 2009-08-05
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to find an appropriate synthesis method to combine fluorophores and nitroxide radicals. Sometimes this synthesis is difficult and the synthesis conditions are relatively harsh. The resulting fluorophore and nitroxide radicals are combined Difficult to purify and preserve

Method used

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  • Complexes used for detecting the reaction for cleaning free radical with anti-oxidants and detection method there of
  • Complexes used for detecting the reaction for cleaning free radical with anti-oxidants and detection method there of
  • Complexes used for detecting the reaction for cleaning free radical with anti-oxidants and detection method there of

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, preparation anionic conjugated polymer PFP-SO 3 - Complex I with cationic radical CAT1

[0037] In 2970uL of phosphate buffer solution with an ionic strength of 5mM, add 3uL of PFP-SO with a concentration of 1mM 3 - (Refer to literature: Burrows, H.D.; Lobo, V.M.M.; Pina, J.; Ramos, M.L.; de Melo, J.S.; Valente, A.J.M.; Tapia, M.J.; Pradhan, S.; -7427 synthesis, or direct purchase), and then add 1.0uL cationic radical quencher CAT1 with a concentration of 30uM, and mix well to obtain complex I with a molar ratio of 1:0.01.

[0038] The present invention uses ultraviolet spectrometer Jasco V-550 type, and fluorescence spectrometer is F-4500 type (Hitachi), is excitation light source with xenon lamp; Used fluorescent cell is the polystyrene fluorescent cell of 3mL, and excitation wavelength detects under the condition of 376nm , all experiments were carried out in phosphate buffer solution (5mM, pH 7.4), the water used was purified by Millipore filtratio...

Embodiment 2～12

[0039] Embodiment 2~12, preparation anionic conjugated polymer PFP-SO of the present invention 3 - Complexes II~XII with cationic radical CAT1

[0040] According to the preparation method and detection method of Example 1, a series of complexes of the present invention were prepared. The anionic conjugated polymer, the molar ratio with the cationic radical quencher, and the fluorescence intensity of the complex are listed in Table 1.

[0041] Table 1, composition and fluorescence intensity of complexes of the present invention

[0042]

Embodiment 13

[0043] Example 13, using the complex in Example 1 to detect the reaction of antioxidants to scavenge free radicals

[0044] 1) In 2970uL ionic strength of 5mM phosphate buffer, add 3uL concentration of 1mM anionic conjugated polymer PFP-SO in the complex of Example 1 3 - , under this concentration condition (1uM) PFP-SO 3 - The fluorescence intensity is 695;

[0045] 2) Then add the cationic radical quencher CAT1 in Example 1, the molar ratio of the added anionic conjugated polymer to the cationic radical quencher is 1:0.01; detect the fluorescence intensity of the formed complex 497( Such as figure 1 shown, the second curve from top to bottom);

[0046] 3) Add 1.5uL of 10uM ascorbic acid (antioxidant) to be tested, mix well and let stand at room temperature for 10 minutes, then detect the fluorescence intensity of the complex at 530 on a fluorometer; compare the change of the fluorescence intensity of the complex before and after adding ascorbic acid, It can be obtained...

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Abstract

The invention relates to a complex used for detecting the free radical scavenging reaction of antioxidants and a detection method thereof. The complex is formed by an anionic conjugated polymer shown in formula I and a cationic radical quencher at a molar ratio of 1:0.01 to 200: see formula I on the right, and the cationic radical quencher is 4-tri Methylammonium iodide-2,2,6,6-tetramethylpiperidin-1-oxyl radical. The method for using the complex to detect the reaction of antioxidants to scavenge free radicals comprises: detecting the fluorescence intensity of the anionic conjugated polymer in the complex of the present invention in phosphate buffer; adding the cationic free radical in the complex of the present invention to quench agent, detect the fluorescence intensity of the complex; add the antioxidant to be tested, and detect the change of the fluorescence intensity of the complex. The invention does not need to synthesize fluorophores and nitrogen-oxygen free radical conjugates, the detection method has high sensitivity and reliability, and can detect the hydrogen transfer process from antioxidants to free radicals and the process of free radicals being reduced by antioxidants.

Description

technical field [0001] The invention relates to a complex used for detecting the free radical scavenging reaction of an antioxidant and a method for detecting the free radical scavenging reaction of an antioxidant using the complex. Background technique [0002] In recent years, research on free radicals in polymer materials, food and biological systems has attracted more and more attention. Free radicals can degrade polymers and foods, and cause oxidative damage to DNA, proteins, and lipids, resulting in a series of diseases such as cancer. Usually, antioxidants are used as free radical scavengers, and the mechanism of trapping free radicals by hydrogen transfer or redox reaction is used to scavenge free radicals, so as to prevent harmful free radical reactions. [0003] When antioxidants are used to scavenge free radicals, the reaction is usually monitored by detecting changes in the ESR signal of free radicals, or by detecting changes in the fluorescence intensity of flu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/00
Inventor 王树唐艳丽
Owner INST OF CHEM CHINESE ACAD OF SCI
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