Method for detecting mutation of gene point through combination of amplification of equipmential specificity and Nano metal probe

Abstract

A method for deteting the mutation at gene site by combining allelic specific amplification with nano-gold probe inclues designing the allelic specific primer complementary with mutant gene but not with wild gene, allelic specific amplifying to obtain double-stranded DNA (product A) and residual single-stranded DNA-primer (product B), and sequentially adding said product and electrolyte solution to nano-gold sol. The color of solution is changed from red to blue for the product A or its not changed for the product B.

Description

technical field [0001] The invention relates to a gene point mutation detection method for gene point mutation detection and single nucleotide polymorphism analysis, which combines allele-specific amplification and nanometer gold probes. Background technique [0002] At present, common methods for detecting gene point mutations include: restriction fragment length polymorphism, denaturing gradient gel electrophoresis, chemical mismatch cleavage, enzymatic mismatch cleavage, single-strand conformational polymorphism, allele-specific probe hybridization, Ligase chain reaction and competitive oligonucleotide primers, etc. Most of these traditional detection methods based on electrophoretic analysis have the disadvantages of complex operation, time-consuming, and the need to use radioactive elements or harmful substances such as ethidium bromide. Contents of the invention [0003] The object of the present invention is to provide a gene point mutation detection method for gen...

AI Technical Summary

Problems solved by technology

Most of these traditional detection methods based on electrophoretic analysis have the disadvantages of complex operation, time-consuming, and the need to use radioactive elements or harmful substances such as ethidium bromide