Kit for predicting birth safety before people-assisted reproduction blastosphere implantation

A technology of human assisted reproduction and assisted reproduction, which is applied in the field of birth safety prediction kits, which can solve the temporal and spatial abnormalities of translation and protein expression, maternal mortality, increased risk of IVF adult disease, and unclear pathophysiological mechanisms And other issues

Active Publication Date: 2015-04-29
BEIJING JISHUITAN HOSPITAL
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  • Claims
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Problems solved by technology

In 2013, Pinborg proposed what is the pregnancy outcome of single embryo transfer in assisted reproduction? A meta-analysis was conducted on the pregnancy outcomes of single-fetal transfer, and it was found that even if the influence of multiple-fetal transfer was excluded, the pregnancy outcomes of assisted reproductive single-fetus transfer were still worse than those of natural pregnancy, including premature birth, intrauterine growth retardation, and low-birth-weight infants. , pre-eclampsia, etc., may be related to the use of ovarian stimulation drugs and germ cell manipulation in vitro, but the deep pathophysiological mechanism is unclear
Due to the application of drugs, in vitro operations and in vitro culture, the d...

Method used

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  • Kit for predicting birth safety before people-assisted reproduction blastosphere implantation
  • Kit for predicting birth safety before people-assisted reproduction blastosphere implantation
  • Kit for predicting birth safety before people-assisted reproduction blastosphere implantation

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Experimental program
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Embodiment

[0120] The research period is from 2010 to 2014. The research content and specimen collection were approved by the Ethics Committee of Peking University, and both the father and mother of the blastocyst were volunteers who gave informed consent. The samples of the research group (a total of 3 research samples, that is, 3 blastocysts) are the 5th day of fertilization and pre-implantation blastocysts collected clinically in assisted reproductive technology.

[0121] For photos of the study group samples see figure 1 . Samples from the research group were taken and mechanically separated under a high-resolution microscope to obtain the inner cell mass that will develop into embryos in the future (see figure 2 ) and the trophectoderm that will develop into the placenta in the future (see image 3 ), and then single cells were taken and single-cell sequencing was performed by Boao Biotechnology Co., Ltd. using the Illumina HiSeq2000 system to obtain the expression of each gene ...

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Abstract

The invention discloses a kit for predicting birth safety before people-assisted reproduction blastosphere implantation. The kit comprises products, wherein the products are used for detecting the expression quantity of 97 genes shown in the table 5 and from a cell of a trophectoderm of a people-assisted reproduction blastosphere. If the expression quantity of the 97 genes from the cell of the trophectoderm of a people-assisted reproduction blastosphere meets the evaluation standard, the people-assisted reproduction blastosphere can develop normally and a pregnant woman can have eutocia; otherwise, the people-assisted reproduction blastosphere cannot develop normally, the possibility of affecting baby health and occurring perinatal complication is increased along with the increase of the number of the genes not meeting the evaluation standard, and the transplantation strategy and decision-making are further affected. With adoption of the kit, the possibility of reducing the perinatal complication and the number of babies suffering from illnesses is reduced.

Description

technical field [0001] The invention relates to a kit for predicting birth safety before implantation of blastocysts from human assisted reproduction. Background technique [0002] Assisted Reproductive Technology (Assisted Reproductive Technology, ART) has helped more than 85% of infertile couples to successfully conceive. In the past 30 years, assisted reproductive technology has undergone ovarian stimulation, in vitro fertilization-embryo transfer (IVF-ET), egg cells A series of developments such as intraplasmic sperm injection (ICSI), preimplantation genetic diagnosis (PGD), in vitro culture of immature eggs (IVM) have made great contributions to the treatment of various infertility. However, since assisted reproductive technology is the first human technology that is directly applied to clinical treatment without clinical safety evaluation, the safety of assisted reproductive technology has been widely concerned. Epidemiological surveys show that after excluding infert...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 乔杰赵亮
Owner BEIJING JISHUITAN HOSPITAL
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