Total artificial propagation method of opsariichthys bidens

An artificial reproduction and artificial technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of affecting the effect of artificial reproduction, failure to achieve fertilization effect, poor synchronization of sexual maturity, etc., and achieve operability and practicability. Strong, good oxytocin effect, high oxytocin rate effect

Inactive Publication Date: 2018-03-23
NINGBO UNIV
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AI Technical Summary

Problems solved by technology

[0004] The above artificial propagation techniques have the following deficiencies or deficiencies: 1. The broodstock used for artificial propagation are all wild echinacea caught in reservoirs and rivers, and the synchronization of sexual maturity between individuals is poor, which affects the effect of artificial reproduction; and wild fish are used as broodstock for breeding Fish fry can only be said to be a "semi-artificial reproduction" method rather than "full artificial reproduction"
②Although artificial oxytocin is used in combination with several hormones, the effect of oxytocin is average
③In artificial insemination, sperm are activated in clear water to fertilize eggs, but fertilization is not carried out in normal saline, which can increase the activation rate of sperm and prolong the motility time of sperm, and the best fertilization effect cannot be achieved
④Hatching of fertilized eggs (hatching in hatching buckets and net cages) is carried out under the condition of uncontrolled natural water temperature. During the hatching period, the water temperature is unstable due to the influence of temperature changes, which affects the hatching effect to a certain extent

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  • Total artificial propagation method of opsariichthys bidens

Examples

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Embodiment 1

[0049] A kind of full-artificial propagation method of horse mouth fish, comprises the following steps:

[0050] (1) Broodstock selection and cultivation

[0051] At the beginning of March, 200 female fish and 130 male fish were selected from the first-generation artificial breeding population (2-year-old fish) that were artificially domesticated and bred by wild horse mouth fish. 60-90g / tail. At the beginning of April, the selected broodstock will be cultivated in the indoor breeding pond (cement pond, area 50-80m2) of the nursery. 2 , water depth 0.6-0.8m) to strengthen cultivation. Disinfect the fish with 0.01g / L potassium permanganate for 10 minutes before entering the pond, and breed at a density of 4-7 fish / m 2 ; The water for cultivation is clean fresh water (stream water or reservoir water), adopting the running water cultivation method, and the daily water exchange volume is 200-300%; the feed for broodstock is compound feed (more than 35% protein content) and Tene...

Embodiment 2

[0060] A kind of full-artificial propagation method of horse mouth fish, comprises the following steps:

[0061] (1) Broodstock selection and cultivation

[0062] At the beginning of March, 200 female fish and 160 male fish were selected from the second-generation artificial breeding population (third-year-old fish) artificially domesticated and bred by wild horse mouth fish. The weight of male fish is required to be 90-130g / tail, and the weight of female fish 70-120g / tail. At the beginning of April, the selected broodstock will be cultivated in the indoor breeding pond (cement pond, area 50-80m2) of the nursery. 2 , water depth 0.6-0.8m) to strengthen cultivation. Disinfect the fish with 0.01g / L potassium permanganate for 10 minutes before entering the pond, and breed at a density of 4-7 fish / m 2 ; The water for cultivation is clean fresh water (stream water or reservoir water), adopting the running water cultivation method, and the daily water exchange volume is 200-300%;...

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Abstract

The invention discloses a total artificial propagation method of opsariichthys bidens. According to the method, parent fish is selected from first-generation and second-generation artificially cultivated groups of wild opsariichthys bidens subjected to artificial domestication and breeding, artificial cultivation is performed until opsariichthys bidens gonad develops maturely, and therefore the parent fish individuals have good maturation synchronism, and the maturation rate is higher; then hormone HCG is used for spawning induction, the spawning induction effect is good, and the spawning induction rate is high; physiological saline is used for activating spermatozoa during artificial insemination, the fertilization effect is improved, and the fertilization rate is higher; and fertilized eggs are put in an incubation frame in an incubation device, hatching is carried out under temperature control conditions, and therefore the hatching effect is improved and a high hatching rate is achieved. According to the method of the present invention, improvement and innovation are carried out on the prior art to realize the full artificial propagation of the opsariichthys bidens. The method focuses on technical links and refinement of technical parameters so as to facilitate popularization and use. The technical method of the present invention is verified by multiple production, and the operability and practicality are high.

Description

technical field [0001] The invention relates to the technical field of artificial propagation of freshwater fish, in particular to a method for fully artificial propagation of larvae. Background technique [0002] The horse mouth fish (Opsariichthys bidens) belongs to Cyprinidae and the horse mouth fish genus, a small economic fish that lives in streams. In my country, it is distributed in various water systems from Heilongjiang in the north to Hainan in the south, and it mostly lives in rapids and shoals, mountain streams and reservoirs. Due to the pollution of the water environment, overfishing and other reasons, the natural resources of the horse mouth fish have declined, so the protection of its resources and the development of breeding technology have been paid attention to. Bakou fish meat is tender and delicious, rich in nutrition, and is very popular among consumers. The market price in Zhejiang is stable at 100-120 yuan / kg. In recent years, horse mouth carp has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 张清科竺俊全张玉明夏荣兴
Owner NINGBO UNIV
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