Culture method of dendritic cells and dendritic cells
A dendritic cell and culture method technology, applied in the field of dendritic cell and dendritic cell culture, can solve the problems of low induction maturation rate, low cell proliferation rate, poor antigen presentation performance, etc. rate and maturation rate, promoting improvement, and improving the effect of antigen presentation
Inactive Publication Date: 2016-06-01
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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Abstract
The invention relates to a culture method of dendritic cells. The culture method includes the following steps that a single karyocyte of a dendritic cell precursor is obtained, culturing is conducted for 4-7 days through a first inductive agent, and immature dendritic cells are obtained; then, a second inductive agent is added to culture the immature dendritic cells till the dendritic cells are mature; the first inductive agent comprises rhGM-CSF and rhIL-4; the second inductive agent comprises rhTNF-alpha, tomato fat-soluble extract or tomato water-soluble extract. The invention further relates to the dendritic cells obtained through the method. Compared with the prior art, the method improves cell activity of the dendritic cells, the value-added ratio and maturing rate of the dendritic cells are raised, and thus the antigen presentation property of the dendritic cells is improved.
Application Domain
Culture processBlood/immune system cells +1
Technology Topic
Rhgm csfWater soluble +5
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