A solid fermentation method for producing the probiotic agent of live Clostridium butyricum

A clostridium, solid fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problems of unreusable raw materials, high production costs, complex processes, etc., and achieves low production costs. Low cost and simple operation

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007]The previous fermentation methods of Clostridium butyricum mainly have the following problems and deficiencies: (1) Using anaerobic culture tanks or maintaining the fermentation by continuously feeding carbon dioxide or nitrogen It is carried out in an anaerobic environment, the raw materials cannot be reused, the operation is more complicated, and the cost is higher; (2) Tryptone, yeast extract, beef extract and its enzymatic hydrolyzate are used as the main nutrients of the medium, and the production cost is low. (3) The post-processing of the liquid fermentation product requires high-speed refrigerated centrifuge for centrifugation, the process is complex and the yield is low; (4) The viable bacterial density of the fermentation product is 1~1.5×109 CFU / mL

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The technical process of producing Clostridium butyricum culture by solid fermentation

[0026] 1. Activation and value-added of bacteria:

[0027] The present invention adopts Clostridium butyricum ( Clostridium butyricum ) as a fermentation strain, strain number: 1.336, purchased from China General Microorganism Culture Collection and Management Center; pick a single colony of Clostridium butyricum from the plate, and inoculate it into 20mL Clostridium seed culture medium (tryptochemical Peptone 10g / L, yeast extract 5g / L, beef extract 5g / L, glucose 10g / L, CaCl 2 0.2 g / L, MgSO 4· 7H 2 O 0.4 g / L, MnSO 4· h 2 O 0.5g / L, cysteine ​​hydrochloride 0.25 g / L, adjusted to pH 7.0, high temperature and high pressure sterilization for standby) in a test tube, 37 ℃ deep static culture for 48 hours, the cultured bacterial solution was treated in 80 ℃ water bath 10min to kill the vegetative body and activate the germination of spores. The treated bacterial s...

Embodiment 2

[0035] Embodiment 2 is different from above-mentioned embodiment 1:

[0036] 1. Medium formula:

[0037] 2. Adjust the pH of the medium during the dissolution process to: 7.4;

[0038] 3. The conditions for heating and sterilizing the solid medium in the fermenter are: 115°C, 30min;

[0039] 4. Inoculation of Clostridium butyricum seed bacteria: After cooling down to 35°C in the fermenter, first add inorganic salt, stir evenly during the addition, then add liquid paraffin to a thickness of 1cm, and insert Clostridium butyricum liquid seed bacteria The proportion of Clostridium butyricum was 8%, and the seed bacteria of Clostridium butyricum were purchased from China General Microorganism Culture Collection Management Center, No.: 1.336, but not limited to the Clostridium butyricum species of this preservation number;

[0040] 5. Temperature-controlled fermentation: control the pH to 7.2, and ferment at 30°C for 68 hours;

[0041] 6. Determination of the viable bacterial den...

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Abstract

The present invention relates to a method for producing a probiotic agent of live Clostridium butyricum, belonging to the field of microbial technology and specially relates to a solid fermentation method for producing the probiotic agent of live Clostridium butyricum. The method comprises the steps of: employing the clostridium butyricum as a fermentation strain which is inoculated on an optimized solid fermentation medium, fermenting under conditions of isolation from air and a temperature of 35-42 DEG C for 48-72h, drawing off and determining a viable cell density, drying, crushing, and packaging to finished products. The method of the invention is advantageous in that: the production process is simple and stable, and easy for promotion; soybean meal and corn flour are used as main ingredients for the medium, greatly reducing the cost of production; the viable cell density is high, the maturation rate of spores is high, stable product quality; and the product quality is stable; and the fermentation product can be directly dried for use without purification, reducing costs and improving the product quality.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing a probiotic feed additive through anaerobic fermentation of Clostridium butyricum on a solid medium. Background technique [0002] Clostridium butyricum ( Clostridium butyricum ), also known as butyric acid bacteria, belongs to the Bacillus family and the genus Clostridium. This bacterium exists in the intestines of soil, animals and human body. The optimum conditions for growth and development of this bacterium are at 37°C and PH 7.0. It can utilize a variety of sugars, such as glucose, lactose, maltose, sucrose and fructose, etc. And can use starch, the main metabolites are butyric acid and acetic acid. [0003] Since 1944, when Butyric acid bacteria preparations were officially put into clinical application as intestinal preparation drugs, Butyric acid bacteria preparations have been used as therapeutic and general medicines for more t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/145
Inventor 李帅伟李本涛何宏轩
Owner GUANGZHOU GLAM BIOTECH
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