A kind of detection method, test strip and application of Salmonella enteritidis

Abstract

The invention discloses a method for detecting Salmonella enteritidis, a test strip and its application. The method for detecting Salmonella enteritidis comprises incubating positively charged nitrogen-rich carbon nanoparticles with Salmonella enteritidis in a sample to be detected to obtain a solution to be tested, and then The Salmonella enteritidis bound to the positively charged nitrogen-rich carbon nanoparticles in the test solution is captured by the Salmonella enteritidis monoclonal antibody. The invention only uses one antibody to draw on the nitrocellulose membrane for direct detection, which breaks the traditional sandwich detection method that uses two antibodies at the same time, greatly saves the cost, solves the difficulty of pairing antibodies, and is simpler, more convenient and novel.

Description

technical field

[0001] The invention belongs to the field of biological detection, and specifically relates to a detection method for Salmonella enteritidis, a test strip and an application thereof. Background technique

[0002] Salmonella is a kind of Gram-negative, most of the perinatal flagella, can move, no capsule and spores, short bacilli with blunt ends, the optimum growth temperature is 37 ℃, and can reproduce normally above 20 ℃. Salmonella infection is one of the zoonotic diseases of great significance in public health. Food transmission in summer and autumn is the main route of infection. Diarrhea, fever, headache, abdominal pain, nausea and vomiting generally occur within 12-14 hours after human ingestion Symptoms such as chills and chills are accompanied by symptoms such as fatigue and muscle aches, which can cause death in severe cases. Among them, Salmonella typhimurium, Salmonella choleraesuis and Salmonella enteritidis are the most common. Salmonella is a ...

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