A kind of detection method of Salmonella enteritidis and its detection test strip

Abstract

The invention discloses a detection method and a detection test strip of salmonella enteritidis. Based on a Gram staining principle, crystal violet is used for staining bacteria into bluish violet firstly; then under the action of capillary effect, the stained bacteria are transferred along a nitrocellulose membrane; based on an antigen and antibody specific binding principle, the bacteria are captured by an antibody of a T line so that the T line becomes a visible strong bluish violet color. The bright bluish violet color can be obtained only if the bacteria are simply stained, and any nano-material labeled antibody is not used so that a complicated labeling process is omitted. According to the detection method and the detection test strip of the salmonella enteritidis, only one antibodyis scratched on the nitrocellulose membrane to detect directly and a traditional sandwich detection method adopting two antibodies is broken through, so that the cost is greatly saved and the difficulty of matching the antibodies is solved; the detection method and the detection test strip are simpler, more convenient and more novel.

Description

technical field

[0001] The invention belongs to the field of biological detection, and in particular relates to a detection method for Salmonella enteritidis and a detection test strip thereof. Background technique

[0002] Salmonella is one of the most important food-borne pathogens that cause food infection and food poisoning. People who eat too much live bacteria will produce symptoms such as diarrhea, vomiting, and fever. In severe cases, they will cause sepsis, dehydration, acidosis, anuria, Heart failure, etc., if the first aid is not timely, it will be life-threatening. Salmonella typhimurium, Salmonella choleraesuis, and Salmonella enteritidis are the most common. Salmonella is a test item in many food standards in my country, and it is required not to be detected. Rapid, accurate, sensitive, and simple detection of Salmonella is of great significance in medical hygiene, food hygiene, and animal disease monitoring. To date, several feasible methods for the detecti...

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