123 results about "Salmonella Ploufragan" patented technology

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and/or Escherichia coli and/or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and/or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention discloses a chromogenic medium for detecting salmonella, which belongs to the fields of food safety and clinical microbiological detection. The culture medium contains agar, peptone, beef extract powder, sodium chloride, a surfactant, cholate, an enzyme inducer, an octoate enzyme chromogenic substrate, a beta-galactosidase chromogenic substrate, a hexosaminidase chromogenic substrate and robiocina. The chromogenic medium disclosed by the invention is used for detecting salmonella, has high detection sensitivity and high specificity, and can be used for initially identifying strains directly according to the color of a colony; the chromogenic medium has high operability, and suitable for treating large-reflux samples, and can be used for comprehensively, systematically and accurately detecting and initially identifying salmonella in food production, clinical disease diagnosis and the environment; and a new way is provided for rapid detection of microorganisms.

The invention discloses a salmonella phage LPSE34. The preservation number of the salmonella phage LPSE34 is CCTCC NO 2018121. The phage is a broad-spectrum salmonella phage and can crack drug-tolerant salmonellas, it is identified that the phage belongs to myoviridae, and the phage is named as LPSE34. The phage LPSE34 is stable in titer at the pH of 4-12 and the temperature of 30-60 DEG C. The invention further discloses application of the salmonella phage in food, especially in chicken and ham systems; and in addition, the invention further discloses a compound edible coating film formed bytaking the phage as an antibacterial substance to be combined with sodium alginate, and compared with antibiotics and chemical preservatives, the phage cannot affect the texture and flavor of food andhas the characteristics of high specificity, no residue and safety. The salmonella phage LPSE34 can serve as a bacteriocidal substance/the antibacterial substance to control pollution of food-borne pathogens to the food and is a very promising biological agent for guaranteeing the food safety.

The invention provides a genetic chip and a detection kit related to major pathogenic microorganisms causing infectious diarrhea of human beings, which are mainly specific to 13 types of bacteria including lapactic bacillus coli/shigella, salmonella, comma bacillus, vibrio parahemolyticus, aeromona hydrophila, plesiomonas, virio hollisae, vibrio fluvialis, vibrio mimicus, Wauter's strains, vibriodamsela and vibrio furnissii. The genetic chip comprises a solid-phase carrier and an oligonucleotide probe fixed on the solid-phase carrier, wherein the oligonucleotide probe comprises DNA (Deoxyribonucleic Acid) fragments selected from genes including a gyrB gene, an ITS gene, a dnaJ gene, a toxR gene and the like with remarkable biological evolution advantages or complementary DNA fragments. By adopting the genetic chip and the detection kit provided by the invention, major pathogenic microorganisms causing infectious diarrhea of human beings can be detected. The genetic chip and the detection kit have the characteristics of easiness and convenience for operation, high flux, high accuracy, high repeatability and the like, and can be applied to clinical detection of inspection departments in hospitals.

The invention discloses an attenuated Salmonella pullorum and application thereof. By using gene knockout technology, the in vivo colonization gene and the main virulence gene of Salmonella pullorum are deleted, thus obtaining an dual-gene-deleted attenuated Salmonella pullorum SM091-DED strain; and the microbiological preservation number of the strain is CGMCC NO.4604. The virulence of the attenuated Salmonella pullorum disclosed by the invention is obviously reduced, and the in vivo colonization time is short after the attenuated Salmonella pullorum is inoculated into a host; the herd infection test shows that the attenuated Salmonella pullorum has no horizontal transmission capability; the attenuated Salmonella pullorum provides full cross protection for homotype and allotype high-virulence Salmonella pullorum; and after SPF (Specific Pathogen Free) chicks are immunized, the infection of the high-virulence strain can be effectively eliminated, and the herd infection can not be caused. Thus, the invention provides a safe, fine-immunogenicity and low-virulence Salmonellosis avium attenuated live vaccine for preventing Salmonellosis avium.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum, and Salmonella pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage of the present invention can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum or Salmonella pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the Salmonella bacteria. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

The invention discloses a salmonella bacteriophage and an application thereof in medicines for preventing and treating salmonella infection diseases. The bacteriophage is named as SPP11 and is preserved in China General Microbiological Culture Collection Center, wherein the preservation date is Nov.04,2019, and the preservation number is CGMCC No.18868. The bacteriophage SPP11 with a strong cracking spectrum has a strong cracking effect on salmonella, can effectively prevent and control salmonella diseases in poultry farms, reduce positivity of the pullorum disease antibody of chickens, reducediarrhea of chickens caused by salmonella enteritis, and can also be used for salmonella sterilization of feeding environment, feed, drinking water and the like. In addition, the bacteriophage is easy for industrial production, and medicines or disinfectants prepared by the bacteriophage not only have reduced cost, but also have the advantages of environmental friendliness.

The invention discloses a biosensor for detecting salmonella typhimurium and application of the biosensor, and belongs to the technical field of harmful microorganism detection. The biosensor is composed of carbon quantum dots Td-CD modified by a salmonella typhimurium nucleic acid aptamer and magnetic beads Td-MB modified by the salmonella typhimurium nucleic acid aptamer. The salmonella typhimurium nucleic acid aptamer and other nucleic acids are assembled through sequence design to form a DNA regular tetrahedron structure, and then the DNA regular tetrahedron structure is respectively usedfor modifying carbon quantum dots and magnetic beads. The method for detecting salmonella typhimurium by adopting the biosensor is simple and convenient to operate and short in consumed time, complexpretreatment does not need to be carried out on a sample to be detected, and the detection cost is low; the biosensor can effectively amplify signals of salmonella typhimurium, can be used for detecting low-concentration salmonella typhimurium in food, and achieves qualitative and quantitative analysis of salmonella typhimurium.

This invention relates to preparation and application of orally administered recombinant DNA vaccine for accelerating animal growth, which Salmonella typhimurium CSO22/pGMCSF-SS (CCTCC M206141) is containing fusion expression plasmid pGMCSF-SS. Salmonella typhimurium CSO22/pGMCSF-SS is prepared by: cloning GMCSF, fusing with plasmid pcS/2SS containing somatostatin gene to obtain fusion expression plasmid pGMCSF-SS, and transforming Salmonella typhimurium. This invention also discloses the application of Salmonella typhimurium CSO22/pGMCSF-SS in orally administered recombinant DNA vaccine for accelerating animal growth.

The invention discloses separation and application of a high-lysis-rate salmonella phage RDP-SA-17118. The host of the salmonella phage RDP-SA-17118 is salmonella S6, and the phage can form a plaque with a diameter of 2mm-4mm on double plates. Through observation by an electron microscope, the phage has a polyhedral stereosymmetric head which wraps nucleic acid and has a diameter of 70nm; the phage has a tail with a length of 120nm and has a tail sheath; a neck is connected with the head and the tail; and the phage belongs to myoviridae of caudovirales. The phage has a high titer of 10<12> pfu/mL and has good tolerance to temperature and pH value, and the titer still keeps 10<12>pfu/mL or above after continuous passage for 29 times. The phage has a good lysis effect on a host after being diluted by 10<6> times, and after the phage is fed, existence of the phage can be detected in heart, liver, lung, kidney, thymus and serum. Through animal experiments, the phage has a good effect in treating chicken salmonella diseases.

The invention relates to a multiplex amplification internal standard PCR (polymerase chain reaction) kit capable of simultaneously detecting Vibrio parahaemolyticus, Escherichia coli O157:H7, Salmonella and Shigella. The kit comprises a PCR reaction premix solution and a reference substance. The detection method comprises the following steps: acquiring a sample, carrying out amplification culture, extracting DNA (deoxyribonucleic acid), preparing a PCR reaction tube, putting the PCR reaction tube on a PCR instrument, carrying out PCR amplification, and carrying out detection result judgment on the PCR amplification product. The kit can simultaneously and quickly detect the four bacteria by one reaction, and can avoid the misjudgment caused by the false negative result due to the inhibiting factor for the DNA polymerase in the sample treatment process. Compared with the existing detection methods, the method provided by the invention is convenient to use, has the advantages of high reliability, short detection period, high sensitivity, high specificity, low cost and simple operation steps, is suitable for high-flux operation and standard operation, and is beneficial to enhancing the food safety detection level and preventing and controlling the foodborne diseases.

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

The invention discloses application of pyroptosis-associated protein GSDMD (Gasdermin-D) for preparing a bacterial ghost vaccine. A bacteriolysis plasmid containing a pyroptosis-associated protein GSDMD coding gene is constructed and is converted into salmonella enteritidis; under the induction of gum sugar, bacteria are split to successfully obtain a salmonella novel bacterial ghost vaccine. An experiment proves that GSDMD mediated bacteriolysis has an extremely long persistent period, and a splitting rate is 99.9985% or more. After a novel bacterial ghost immune mouse prepared by the invention is adopted, an organism can be stimulated to generate powerful humoral immunity and cellular immunity; protective immunity response is induced; an experiment animal can be protected so as to resistsalmonella infection. The salmonella bacterial ghost vaccine prepared by the invention has a good immune protection effect.

The invention belongs to the technical field of biology, and particularly relates to a plastic-packaged colloidal gold detection card for detecting salmonella toxin and a preparation method of the plastic-packaged colloidal gold detection card. The detection card comprises a test strip, the test strip comprises a back plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and a waterabsorption pad, the sample pad, the colloidal gold pad, the nitrocellulose membrane and the water absorption pad are sequentially fixed on the back plate, and the colloidal gold pad contains a monoclonal antibody of a colloidal gold labeled salmonella toxin antigen; a detection line and a quality control line are arranged on the nitrocellulose membrane. Through the implementation of the process,a rapid production and preparation technology of the salmonella toxin rapid immunodetection card is developed.

The invention discloses a quick detection method by using an interior label and taking a sigD/sigE gene of salmonella as a target through reverse transcription fluorescent quantitation PCR (Polymerase Chain Reaction). In the quick detection method, an RNA (Ribonucleic Acid) interior label obtained by PCR amplification for three times is composed. In addition, the invention further provides a corresponding kit and application. The method disclosed by the invention can be widely applied to the fields such as research of laboratories, food safety and health and hygiene. The phenomenon of false negative missed detection can be avoided, so that the PCR amplification is effectively monitored; in addition, the detection specificity of salmonella is strong, so that dead bacteria and live bacteriacan be effectively distinguished; the quickness and high detection sensitivity are obtained; and the method can be applied to high-throughput screening of the salmonella in the food.

The invention discloses specific novel molecular targets of 16 different serotypes of salmonella and a rapid detection method of the specific novel molecular targets. The invention provides a method for detecting salmonella enteritidis, which is used for different serotypes of salmonella. The invention method provides 72 specific molecular detection targets for different serotypes of salmonella including salmonella enteritidis, salmonella typhimurium, salmonella derby, salmonella indiana, salmonella agona, salmonella infantis, salmonella rissen, salmonella braenderup, salmonella virehow , salmonella albany, salmonella hadar, salmonella weltevreden, salmonella London, salmonella meleagridis, salmonella senftenberg and salmonella pomona, and corresponding PCR primer groups and a rapid detection method. The method provided by the invention has the advantages of short detection time, low cost, simple operation and strong specificity; meanwhile, the detection method can be used for detecting some strains with unexpressed antigens, avoiding the defects of immunodetection, and is more accurate in detection result, simpler in judgment and higher in practicability.

The invention discloses a specific primer for detecting salmonella pullorum, a kit with the primer and application of the primer. Aiming at a specific fragment of salmonella pullorum, the primer for specific proliferation of salmonella pullorum is designed, and a PCR detection method capable of directly detecting salmonella pullorum in an excrement sample is established, the method is good in specificity, high in sensitivity and short in detection time, salmonella pullorum can be quickly and accurately detection from the excrement sample, but proliferation results of 19 kinds of other common pathogenic salmonella serotypes and 7 kinds of common non-salmonella pullorum are all negative. Thus, according to the provided specific primer for detecting salmonella pullorum, the kit with the primer and application of the primer, a fast and effective technical means is provided for detection of salmonella pullorum, moreover, the method is easy to operate and low in requirement for equipment, the whole detection process can be finished in only one day, and requirements of most poultry breeding plants on fast detection of salmonella pullorum can be met.

The invention relates to the technical field of probiotic preparations for resisting pathogenic bacterium infection in livestock breeding, and particularly discloses a complex probiotic preparation for resisting salmonella pullorum infection and application of the complex probiotic preparation. The complex probiotic preparation comprises bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens, clostridium butyricum and lactobacillus plantarum. The preparation specifically improves the body's resistance to salmonella pullorum infection, significantly improves intestinal mucosal immunity, reduces or avoids intestinal diseases, maintains intestinal microecological balance, reduces the addition of the long-term subtherapeutic dose and ultra-high dose of antibiotics for preventing and treating animal diseases in feed, and extremely has promoting significance.

The invention discloses a method for rapid detection of Salmonella typhimurium based on the photothermal effect of an immunomagnetic nanomaterial. According to the method, a standard curve for the values of temperature rises and the numbers of Salmonella typhimurium is established by using the photothermal effect of an immunomagnetic nanomaterial, and the value of the temperature rise of a samplecan be substituted into the standard curve so as to obtain the number of Salmonella typhimurium in the sample; and the three links consisting of enrichment and separation, detection and sample inactivation are effectively integrated by using the magnetic and photothermal effect of the immunomagnetic nanomaterial. The method provided by the invention is sensitive, safe, rapid, portable and cheap; experimental results verify that the method has a minimal detection limit of 300 bacteria and the temperature rise value delta T is linearly related to the Salmonella typhimurium number in a range of 300-1000; therefore, the method provided by the invention has good practical application prospects and can be applied to actual rapid detection of Salmonella typhimurium.

The invention discloses a salmonella choleraesuis immune colloidal gold rapid testing strip. The salmonella choleraesuis immune colloidal gold rapid testing strip comprises a sample pad, a colloidal gold pad, a NC membrane, and an absorbent pad which are connected successively; monoclonal antibody Mab05-D10, which is generated by a hybridoma cell strain with a preservation number of CGMCC No.7710, is used as gold-labeled antibody coupled colloidal gold, and is sprayed onto the colloidal gold pad; and monoclonal antibody Mab05-F10, which is generated by a hybridoma cell strain with a preservation number of CGMCC No.7712, is used as a detection antibody, and is sprayed onto the NC membrane so as to form detection lines. In addition, the invention also provides applications of the salmonella choleraesuis immune colloidal gold rapid testing strip in detecting food salmonella choleraesuis. It is shown by experiments that detection specificity and sensitivity of the salmonella choleraesuis immune colloidal gold rapid testing strip on salmonella choleraesuis are both relatively high.