Plastic-packaged colloidal gold detection card for detecting salmonella toxin

Abstract

The invention belongs to the technical field of biology, and particularly relates to a plastic-packaged colloidal gold detection card for detecting salmonella toxin and a preparation method of the plastic-packaged colloidal gold detection card. The detection card comprises a test strip, the test strip comprises a back plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and a waterabsorption pad, the sample pad, the colloidal gold pad, the nitrocellulose membrane and the water absorption pad are sequentially fixed on the back plate, and the colloidal gold pad contains a monoclonal antibody of a colloidal gold labeled salmonella toxin antigen; a detection line and a quality control line are arranged on the nitrocellulose membrane. Through the implementation of the process,a rapid production and preparation technology of the salmonella toxin rapid immunodetection card is developed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plastic-encapsulated colloidal gold detection card for detecting Salmonella toxin and a preparation method thereof. Background technique [0002] According to WHO estimates, the under-reporting rate of foodborne diseases in developed countries is more than 90%, while in developing countries it is more than 95%. Based on this inference, the food poisoning data currently available in my country is only the "tip of the iceberg" of foodborne diseases that actually occur in our country. For such a high rate of false positives, in addition to management problems, the limitation of detection and traceability methods of pathogenic microorganisms is also an important factor. At present, bacterial food poisoning mainly includes two types: infectious poisoning and toxin poisoning. Salmonella and its toxins are a common foodborne pathogen. [0003] At present, my country's food ...

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Problems solved by technology

[0004] Traditionally, the pathogen detection and identification methods of foodborne diseases in my country still remain in the traditional pathogen culture. The biggest weakness of this test method is that it is slow and difficult to adapt to diagnosis and treatment. It mainly includes two methods: one is PCR molecular identification technology to detect pathogens , although the sensitivity is high, fast, and can be detected directly, but the requirements for the laboratory and reagents are relatively high, otherwise the results are very unreliable; secondly, although the enzyme-linked immunoassay (ELISA) detection is a relatively mature immunoassay technology, it is The operation of this method is cumbersome, the detection time is long, and it needs to be carried out in the laboratory, which cannot meet the needs of on-site rapid detection

Therefore, the backwardness of the current detection methods for pathogenic microorganisms limits the ability to trace and identify pathogenic bacteria that cause foodborne diseases to a considerable extent.

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