Kit for simultaneously detecting Vibrio parahaemolyticus, Escherichia coli O157:H7, Salmonella and Shigella

Abstract

The invention relates to a multiplex amplification internal standard PCR (polymerase chain reaction) kit capable of simultaneously detecting Vibrio parahaemolyticus, Escherichia coli O157:H7, Salmonella and Shigella. The kit comprises a PCR reaction premix solution and a reference substance. The detection method comprises the following steps: acquiring a sample, carrying out amplification culture, extracting DNA (deoxyribonucleic acid), preparing a PCR reaction tube, putting the PCR reaction tube on a PCR instrument, carrying out PCR amplification, and carrying out detection result judgment on the PCR amplification product. The kit can simultaneously and quickly detect the four bacteria by one reaction, and can avoid the misjudgment caused by the false negative result due to the inhibiting factor for the DNA polymerase in the sample treatment process. Compared with the existing detection methods, the method provided by the invention is convenient to use, has the advantages of high reliability, short detection period, high sensitivity, high specificity, low cost and simple operation steps, is suitable for high-flux operation and standard operation, and is beneficial to enhancing the food safety detection level and preventing and controlling the foodborne diseases.

Description

technical field [0001] The invention relates to biotechnology, in particular to a kit capable of simultaneously detecting multiple systems of Vibrio parahaemolyticus, Escherichia coli O157:H7, Salmonella and Shigella, which can simultaneously identify and detect samples containing the above four bacteria. Background technique [0002] Foodborne disease covers a very wide range and is a serious public health problem in both developed and developing countries around the world. Surveillance data show that in 2011, 1 out of 6.5 people in China suffered from foodborne diseases. The global incidence of foodborne illness is difficult to estimate, but a reported 1.8 million deaths were attributed to diarrheal diseases in 2005 alone. The majority of these cases are attributable to food and drinking water contamination, with foodborne pathogens being the main cause. [0003] On September 25, 2012, the Ministry of Health announced the draft of "Limits of Pathogenic Bacteria in Food",...

AI Technical Summary

Problems solved by technology

Traditional biological methods take a long time and have complicated detection steps. Colloidal gold test strips are relatively expensive and have low detection sensitivity. Enzyme-linked immunosorbent assay (ELISA) is expensive and requires certain skills in operation. Simple, rapid, sensitive and specific detection method, especially for high-throughput, rapid and simultaneous detection of a large number of samples

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