Immunosorbent for removing inflammatory factors in blood and preparation method thereof

An immunoadsorbent and inflammatory factor technology, applied in the field of biomedicine, can solve the problem of low selectivity, achieve high adsorption performance, avoid non-specific adsorption and low cost.

Active Publication Date: 2018-11-23
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these two adsorbents have achieved good adsorption effects in vitro, they mainly rely on hydrophobic interactions to remove toxins, which has the disadvantage of low selectivity, and may remove toxins while also removing beneficial substances in the body.

Method used

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  • Immunosorbent for removing inflammatory factors in blood and preparation method thereof
  • Immunosorbent for removing inflammatory factors in blood and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of polystyrene microspheres

[0024] Add monomer 53g vinyl acetate and crosslinking agent 10g triallyl isocyanurate into a 250mL beaker and mix well, then add 35.2g ethyl acetate, 35.9g n-heptane, 23.4g n-hexane The alkane and 0.6 g of benzoyl peroxide are stirred to dissolve them. After the dissolution is complete, they are mixed thoroughly to obtain an oil phase. Then the system solution was added to 252mL 2.8% polyvinyl alcohol solution (with 1.2% NaCl), the stirring rate was adjusted to disperse the system into uniform small oil beads, after the temperature was raised to 52°C, after the reaction for 5.5h, Then continue to heat up to 79℃ to react for 3.5h, filter, the reaction system is filtered and washed with hot water after treatment, then extracted with methanol for 16h to remove the porogen, vacuum dried for 36h to obtain white copolymer microspheres and added to 1.2% In the NaOH / methanol solution, the transesterification was carried out at 40°C fo...

Embodiment 2

[0030] (1) Preparation of polystyrene microspheres

[0031] Add monomer 62.6g vinyl acetate and crosslinking agent 17.4g triallyl isocyanurate into a 500mL beaker and mix well, then add 84.5g ethyl acetate, 153.6g n-heptane, 145.9 g n-hexane and 4.64 g of benzoyl peroxide are stirred to dissolve them, and after the dissolution is complete, they are mixed thoroughly to obtain an oil phase. Then the system solution was added to 1299.2mL 0.65% polyvinyl alcohol solution (containing 9.6% NaCl), the stirring rate was adjusted to disperse the system into uniform small oil beads, after the temperature was raised to 59°C, after 3.5 hours of reaction, Then continue to heat up to 72℃ to react for 5h, filter, the reaction system is filtered and washed with hot water after treatment, then extracted with methanol for 28h to remove the porogen, vacuum dried for 24h to obtain white copolymer microspheres and added to 4.9% In NaOH / methanol solution, the transesterification was carried out at 60...

Embodiment 3

[0037] (1) Preparation of polystyrene microspheres

[0038] Add monomer 49.2g vinyl acetate and crosslinking agent 30.8g triallyl isocyanurate into a 500mL beaker and mix well, then add 29.6g ethyl acetate, 118.2g n-heptane, 92.2 g n-hexane and 2.08 g of benzoyl peroxide are stirred to dissolve, and after the dissolution is complete, mix well to obtain an oil phase. Then add the system solution to 740.8 mL of 1.7% polyvinyl alcohol solution (with 5.2% NaCl), adjust the stirring rate to disperse the system into uniform small oil beads. After the temperature is raised to 55°C, the reaction time is 4.5 hours. Then continue to heat up to 76℃ to react for 4.5h, filter, the reaction system is filtered and washed with hot water after treatment, and then extracted with methanol for 36h to remove the porogen, vacuum dried for 12h to obtain white copolymer microspheres and added to 2.6% In the NaOH / methanol solution, the transesterification was carried out at 50°C for 48h, filtered, extra...

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Abstract

The invention discloses an immunosorbent for removing inflammatory factors in blood and a preparation method thereof. Based on a variety of intermolecular interactions between the antigenic epitope ofinflammatory factors such as IL-6, TNF-alpha, IL-1 beta and IL-8 and corresponding receptors thereof, affinity ligand of small molecule polypeptide with 5-18 selective amino acids is designed througha computer-assisted drug design method. Specifically, with vinyl acetate-triallyl isocyanurate as a basic skeleton, a mixture of ethyl acetate and alkane as a pore-forming agent, and a synthetic resin with benzoyl peroxide as an initiator as a carrier, alcoholysis activation is carried out, and then the small molecule polypeptide is grafted to obtain the small-molecule ligand immunosorbent whichdoes not adsorb macromolecular proteins and the like. The immunosorbent is simple to prepare, has high adsorbing capacity, high selectivity, good biological and blood compatibility, and relatively lowcost, and provides a new therapeutic method for removing excessive pathogenic inflammatory factors in patients.

Description

Technical field: [0001] The invention belongs to the field of biomedicine technology. Specifically, it relates to a novel medical immunosorbent, especially an immunosorbent suitable for hemoperfusion to remove inflammatory factors from the body and a preparation method thereof. technical background: [0002] Inflammation is one of the most basic pathological changes. During the development of inflammation, it can stimulate the body's inflammatory cells (such as neutrophils, macrophages, endothelial cells, etc.) to release a variety of cytokines, thereby causing systemic inflammatory responses. It even causes sepsis and septic shock. More than 18 million people worldwide suffer from sepsis each year. In our country, due to the abuse of antibiotics, poor air quality, water pollution, etc., there are also millions of patients with sepsis each year. The case fatality rate has surpassed myocardial infarction and has become the leading cause of death for non-heart disease patients in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/30A61M1/36
CPCA61M1/3687A61M2202/0413B01J20/267A61M2202/0021
Inventor 欧来良柴雅敏
Owner NANKAI UNIV
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