Endotoxin adsorption material for curing endotoxemia

Abstract

The invention relates to endotoxin adsorbing material for curing endotoxemia in the bioseparation and the biomedical engineering fields. The adsorbing material which takes sepharose gel as a carrier is coupled with spacer arm molecules through the activating reaction, and then is coupled with two groups of quarternary ammonium salt cations and dewatering molecules as functional groups to be prepared. The adsorbing material is simultaneously coupled with the two groups of functional groups mutually effected with endotoxin, and further the adsorbing selectivity of the adsorbing material to the endotoxin is improved. The physicochemical property of the adsorbing material is steady, and the adsorbing material has good blood compatibility, thereby being able to used for removing the endotoxin in blood plasma, and curing the endotoxemia. The preparation process of the adsorbing material is simple, the preparation cost is low, and the adsorbing material belongs to the novel endotoxin adsorbing material.

Description

technical field [0001] The invention belongs to the fields of biological separation and biomedical engineering, and particularly relates to an endotoxin adsorption material for treating endotoxemia and a preparation method thereof. Background technique [0002] Endotoxin is one of the components of the cell wall of Gram-negative bacteria, which is a complex of lipopolysaccharide and trace protein. Endotoxemia is a condition caused by the release of a large amount of bacterial endotoxin into the blood by bacteria in the blood or in the lesion, or the transfusion of a large amount of endotoxin-contaminated fluid. After endotoxin enters the blood, it causes a series of reactions, eventually leading to organ necrosis, irreversible shock and death. According to reports, about 100,000 patients in the United States die from Gram-negative bacterial infections every year, and there is no effective clinical treatment. [0003] Many drugs have been used in the treatment of endotoxemi...

AI Technical Summary

Problems solved by technology

However, activated carbon and macroporous resin have no selectivity for the adsorption of endotoxin, and they also remove a lot of protein in the blood while removing endotoxin, and the blood compatibility is very poor, and the effect is not obvious

Although the immobilized polymyxin B (Polymyxin B) gel has strong endotoxin adsorption capacity, the amount of protein loss is also obvious, it is expensive, and it has neurotoxicity and nephrotoxicity after ligand shedding, so it is not suitable Not suitable for removing endotoxin from intravenous fluids (Chinese patent, 200410024717.6)

Although the immunoadsorbent material does not affect the level of other components in the blood and has high clearance efficiency, the source, preparation and purification of antibodies are very difficult and expensive. When the antibodies are released from the surface of the material, a series of immune reactions will be caused

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