Automatic fluid device for detecting protein in biological sample and its method

A biological sample and protein technology, applied in biological testing, measuring devices, fluid controllers, etc., can solve the problems of uneconomical, complex structure, expensive automatic detection device, etc., and achieve the effect of simple structure, convenient and fast detection

Inactive Publication Date: 2004-08-04
SAMSUNG ELECTRONICS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the above-mentioned conventional automatic detection device cannot fully automate a series of testing procedures, including injecting the sample, cleaning the channel, injecting the dye, cleaning the channel and detecting the sample.
In addition, the traditional automatic detection device requires a separate energy source and skilled operators due to its complex structure, and the same is true for maintenance and repair equipment
In addition, traditional automatic detection devices are expensive and uneconomical

Method used

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  • Automatic fluid device for detecting protein in biological sample and its method
  • Automatic fluid device for detecting protein in biological sample and its method
  • Automatic fluid device for detecting protein in biological sample and its method

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Embodiment Construction

[0019] An automatic fluid device according to a specific embodiment of the present invention includes a disposable cylinder, a storage tank for compressed air, a storage tank for buffer solution and a detection device.

[0020] The cartridge of the automated fluidic device according to the present invention includes a sample reservoir, control reservoirs and a dye reservoir. Hereinafter, the sample liquid reservoir, the control substance liquid reservoir and the dye liquid reservoir are collectively referred to as the cylinder liquid reservoir 9 .

[0021] A biological sample containing a target protein to be studied is contained in the sample liquid reservoir. Any biological sample, such as blood, urine, cerebrospinal fluid, saliva, and interstitial fluid, in which a protein of interest detectable by enzyme-linked immunosorbent assay (ELISA) is contained can be used.

[0022] The control substance liquid reservoir holds the control substance solution, that is, the target pro...

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Abstract

An automated microfluidic system that includes a cartridge reservoir part, a cartridge, a compressed-air storage tank, a buffer storage tank, and a reader is provided. The automated microfluidic system that detects a particular protein in a biological sample by enzyme-linked immunosorbent assay (ELISA) can automate a series of assaying processes, beginning with sample injection and ending with detection, with a simple structure, thereby allowing for convenient and quick detection of a particular protein in a biological sample without requiring skillful, elaborate manipulations by an operator.

Description

field of invention [0001] The invention relates to a biomolecular detection device, in particular to an automatic fluid device for detecting proteins in biological samples. Background technique [0002] Most diagnostic methods involve a major step of detecting the presence of a specific protein in a biological sample. There are many diagnostic methods that have been widely used, such as enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), fluorescence detection, nuclear magnetic resonance spectroscopy and colorimetric detection, the most commonly used is Enzyme-linked immunosorbent assay. [0003] The detection of specific proteins by ELISA involves three techniques: one is the antigen-antibody chemical reaction technique, in which antibodies that can be immobilized on a matrix or labeled with an enzyme are combined with the target protein to be studied. protein reaction; another is an optical detection method, in which the color change of the matrix caused b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53B01L3/00G01N35/02G01N37/00
CPCB01L2400/0622B01L2300/0867B01L3/5027B01L2200/10B01L3/502723B01L2400/0487G01N35/02
Inventor A·杰伊·德什米克
Owner SAMSUNG ELECTRONICS CO LTD
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