Immunosorbent for rheumatoid factors for blood perfusion and preparation method thereof

A technology of immunosorbent and rheumatoid factor, which is applied in the field of biomedicine, can solve the problems of poor specific adsorption and poor removal effect, and achieve the effects of improving adsorption performance, good biocompatibility, and avoiding non-specific adsorption

Active Publication Date: 2018-09-28
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the adsorbents using hydrophobic amino acids as ligands mainly use hydrophobic force and electrostatic force to remove toxins, and the specific adsorption is relatively poor. While removing toxins, they will also remove total protein and fiber in the patient's blood. Protein, etc.; 1-amino-3-(2-(4-pyridyl))-ethylthiol as the ligand is based on the molecular structure characteristics of IgM. Through computer molecular docking, IgM-type antibodies are screened Ligands with strong binding ability, but this type of adsorbent only has a specific scavenging effect on RF-IgM, and has poor scavenging effects on RF-IgG and RF-IgA

Method used

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  • Immunosorbent for rheumatoid factors for blood perfusion and preparation method thereof
  • Immunosorbent for rheumatoid factors for blood perfusion and preparation method thereof
  • Immunosorbent for rheumatoid factors for blood perfusion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of polystyrene-divinylbenzene carrier microspheres with chloromethylated macroporous structure;

[0033] (1.1) Preparation of polystyrene-divinylbenzene microspheres with macroporous structure

[0034] Add 70g of monomer styrene, 20g of crosslinking agent divinylbenzene; porogen (toluene 29.5g, kerosene 37g and octane 221.5g) and initiator benzoyl peroxide (BPO) 1.79g; into a 2000mL three-necked flask , stir to mix evenly, then add 456g, 2.9% polyvinyl alcohol aqueous solution, adjust the stirring speed at 120-160rpm / min, make the system disperse into uniform small oil droplets, raise the temperature to 52°C, and set the temperature at 5°C / 10min Raise the temperature to 70°C, keep it at this temperature for 8 hours after setting the shape, then slowly raise the temperature to 92°C at a rate of 1°C / 5min, and react for 12 hours. After the reaction system is filtered and washed, polystyrene with macroporous structure is obtained. - divinylbenzene microsphe...

Embodiment 2

[0042] (1) Preparation of polystyrene-divinylbenzene carrier microspheres with chloromethylated macroporous structure

[0043] (1.1) Preparation of polystyrene-divinylbenzene microspheres with macroporous structure

[0044] With monomer styrene 83.3g, crosslinking agent divinylbenzene 16.7g; porogen (toluene 29.6g, kerosene 32.0g and octane 138.4g) and initiator benzoyl peroxide (BPO) 7g; Add to 2000mL In the three-necked flask, stir to mix evenly, then add 840g, 1.4% polyvinyl alcohol aqueous solution, adjust the stirring speed at 120-160rpm / min, make the system disperse into uniform small oil droplets, heat up to 52°C, and / 10min to raise the temperature to 75°C, keep the temperature at this temperature for 6 hours, then slowly raise the temperature to 94°C at a rate of 2°C / 5min, and react for 9.5 hours. After the reaction system is filtered and washed, the macroporous structure is obtained. Polystyrene-divinylbenzene microspheres. The particle size is 400-600μm, and the s...

Embodiment 3

[0051] (1) Preparation of polystyrene-divinylbenzene carrier microspheres with chloromethylated macroporous structure

[0052] (1.1) Preparation of polystyrene-divinylbenzene microspheres with macroporous structure

[0053] Synthesis of carrier microspheres: monomer styrene 120g, crosslinking agent divinylbenzene 20g; porogen toluene 41.8g, kerosene 52.3g and octane 365.9g and initiator benzoyl peroxide (BPO) 9g; add Put it into a 2000mL three-neck flask, stir to mix evenly, then add 600g, 2.5% polyvinyl alcohol aqueous solution, adjust the stirring speed at 120-160rpm / min, make the system disperse into uniform small oil droplets, heat up to 58 °C, and 10°C / 10min to raise the temperature to 80°C, keep at this temperature for 4 hours after setting, then slowly raise the temperature to 95°C at a speed of 2°C / 5min, react for 8 hours, the reaction system is filtered, washed and treated to obtain a macroporous structure polystyrene-divinylbenzene microspheres. The particle size i...

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Abstract

The invention relates to an immunosorbent for rheumatoid factors for blood perfusion and a preparation method thereof. The immunosorbent uses chloromethylation macroporous structure styrene-divinylbenzene resin as carriers, uses micro-molecule polypeptides (consisting of 6 to 14 amino acids) as ligands, uses amino-functionalization polyethylene glycol (H2N-PEG-NH2) with different molecular weightas arms, so that carrier microspheres are coupled with the ligands through the arms. The preparation of the immunosorbent is simple; on the basis of maintaining the specific adsorption on the rheumatoid factors, the relevant side effects of potential immunogenicity and the like of the macromolecule (antigen or antibody) ligand immunosorbent is avoided; the cost is low; the immunosorbent is suitable for being used for the excessive rheumatoid factors in a body of the patient through blood or plasma perfusion.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine. It specifically relates to a novel medical immunosorbent, especially an immunosorbent suitable for hemoperfusion to remove rheumatoid factors in patients and a preparation method thereof. technical background: [0002] As the most common clinical chronic systemic inflammatory autoimmune disease, rheumatoid arthritis (Rheumatoidarthritis, RA) is characterized by joint synovitis as the main clinical feature, which can lead to joint damage and deformity, resulting in joint dysfunction and even disability. . There are millions of patients with rheumatoid arthritis in my country, and the incidence rate is 0.32%-0.38%. Studies have shown that rheumatoid factors (Rheumatoidfactors, RFs) in the blood of patients are the main pathogenic substances, mainly including RF-IgM, RF-IgG and RF-IgA types, of which RF-IgM and RF-IgG account for about 80%. RFs is the most characteristic autoantibody in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28C08J9/36C08J9/14C08F212/08C08F212/36C08F8/24B01J13/14C08G81/00B01J20/26B01J20/30
CPCB01J13/14B01J20/265C08F8/24C08F212/08C08G81/00C08J9/141C08J9/28C08J9/36C08J2325/08C08F212/36
Inventor 欧来良柴雅敏
Owner NANKAI UNIV
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