A hybridoma cell line secreting cpiv3 antibody and elisa kit
A technology of hybridoma cell lines and kits, applied in the field of hybridoma cell lines and ELISA kits
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0020] 1. Antigen preparation: Infect MDBK with CPIV3 virus (JS2013) (Yang L, Li W, Mao L, et al. Analysis on the complete genome of novel caprine parainfluenza virus 3. Infect GenetEvol.2016,38:29-34.) Cells were inoculated for 4 days, and the virus was harvested after cell lesions appeared, and centrifuged at 10,000rpm for 30min to remove cell debris; the supernatant was ultracentrifuged at 40,000g for 2h, the supernatant was discarded, and the precipitate was dissolved overnight with an appropriate amount of PBS.
[0021] 2. Immunization of Balb / c mice: immunize Balb / c mice with multi-point subcutaneous injection of ultra-isolated virus, a total of 3 immunizations, with an interval of 2 weeks between each immunization, and use 100ul of ultra-isolated virus and the same amount of complete Freund's adjuvant for the first immunization Mixed immunization with 100ul ultra-isolated virus and the same amount of incomplete Freund's adjuvant for the last two times; blood was collecte...
Embodiment
[0050] 212 goat clinical serum samples were collected, and the CPIV3 antibody in the serum was detected by the blocking ELISA method and neutralization test established above, respectively. Compared with the results of blocking ELISA and neutralization experiment, the coincidence rate was 98.8%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com