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Recombinant Newcastle disease LaSota vaccine strain for expressing West Nile virus (WNV) PrM/E protein

A technology for West Nile fever and vaccine strains, applied in the direction of virus/bacteriophage, antiviral agents, virus antigen components, etc., can solve the problems of no West Nile virus isolation, no cases of West Nile virus encephalitis, etc.

Inactive Publication Date: 2015-05-20
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] West Nile fever is an important zoonotic disease. No cases of West Nile virus encephalitis have been found in my country, and no West Nile virus has been isolated.

Method used

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  • Recombinant Newcastle disease LaSota vaccine strain for expressing West Nile virus (WNV) PrM/E protein
  • Recombinant Newcastle disease LaSota vaccine strain for expressing West Nile virus (WNV) PrM/E protein
  • Recombinant Newcastle disease LaSota vaccine strain for expressing West Nile virus (WNV) PrM/E protein

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Embodiment Construction

[0039] The present invention will be further described below with reference to specific examples, but those skilled in the art should understand that the present invention is not limited to these specific examples.

[0040] 1. Materials and methods

[0041] 1.1.1: Virus strain and plasmid: Newcastle disease virus LaSota vaccine strain (GenBank accession number AY845400.2), Newcastle disease LaSota vaccine strain reverse genetic operating system was prepared by our laboratory, the preparation method refers to the authorized patent: application number : 200510097997.8 (the name of the invention is "Newcastle Disease LaSota Vaccine Strain Reverse Genetic Operating System and Its Application", inventors: Bu Zhigao, Chen Hualan, application time September 2, 2005), recombinant pox vTF7- 3 Provided by Dr. Bernard Moss of the NIH in the United States, the preparation and titration process was carried out according to the literature [18], and the titrated virus solution was stored at ...

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Abstract

The invention provides a recombinant Newcastle disease virus LaSota vaccine strain rLa-WNV-PrM / E for expressing a West Nile virus (WNV) PrM / E protein. The collection number of the vaccine strain is CGMCC No. 10199; the body fluid generated by the recombinant virus and the cellular immune response are systematically evaluated in a mouse mold. Results indicate that the vaccine strain is capable of correctly expressing the PrM / E protein. A C57BL / 6 mouse is immunized twice by use of the recombined virus, and then an ELISA result indicates that the immunized mouse is capable of generating high-level PrM / E protein-specific IgG; a neutralization test result indicates that the immunized mouse is capable of generating high-level WNV neutralization antibodies; the flow cytometry indicates that the immunized mouse is capable of generating WNVE protein epitope-specific CD4+ and CD8+T cell immune response. The vaccine strain is a safe, efficient West Nile virus prevention and control candidate vaccine having perspectiveness and reservation property, and has great strategic importance for China to cope with the potential threats of the virus.

Description

technical field [0001] The invention provides a recombinant Newcastle disease virus LaSota vaccine strain expressing West Nile virus (WNV) PrM / E protein and application thereof. Specifically, the present invention provides recombinant Newcastle disease virus LaSota vaccine strain rLa-WNV-PrM / E expressing West Nile fever virus PrM / E protein (its preservation number is CGMCC No.10199) and its preparation for preventing West Nile fever virus. Vaccine application for Nile fever. Background technique [0002] West Nile Virus (WNV) is an arbovirus transmitted by Culex mosquitoes. WNV belongs to the Flaviviridae family and is widely distributed in Africa, southwestern Europe, Russia, the Middle East, India and Australia. The virus was introduced to North America in 1999 [1,2]. Serologically, West Nile virus belongs to the Japanese encephalitis serogroup, which includes St. Louis encephalitis virus, Murray Valley encephalitis virus, Japanese encephalitis virus, and Kunjin virus. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01A61K39/12A61P31/14C12R1/93
CPCY02A50/30
Inventor 步志高温志远葛金英华荣虹王喜军
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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