Hybridoma cell line against akabane virus monoclonal antibody, monoclonal antibody as well as reagent kit and uses thereof

Abstract

The present invention discloses an anti-akabane virus monoclonal antibody hybridoma cell system, a monoclonal antibody, a kit and a purpose thereof. A hybridoma technology is used to obtain rat-anti-akabane virus monoclonal antibody, the akabane virus is considered as a detection system target antigen, and the akabane virus antibody of a sample for detecting is detected by a competitive enzyme-linked immunosorbent mensuration method. The whole detection process only needs 2.5 hours and all test steps are processed inside a 96-hole enzyme label plate; compared with the traditional neutralization test, the present invention saves time and labor and has high throughput and good repeatability.

Description

Technical field [0001] The invention relates to a method for detecting antibodies in animal quarantine, in particular to a method for detecting Akabane virus antibodies in the serum of cattle and sheep, namely, a hybridoma cell line of monoclonal antibodies against Akabane virus, monoclonal antibodies and kits thereof. Background technique [0002] Akabane disease (AKAv), also known as Akabane disease, is a polytype infectious disease of cattle, sheep and goats caused by Akabane virus (AKAv). Characterized by miscarriage, premature delivery, stillbirth, and deformity, it can cause congenital joint curvature and watery anencephaly. The disease was prevalent in cattle, sheep and goat herds in Australia in the 1930s, and was subsequently reported in Japan. From 1972 to 1973, unexplained miscarriages, premature births, stillbirths, and congenital joint bending-hydrocephalus anencephal syndrome (AH syndrome) occurred in herds west of Kanto, Japan, and lost more than 50,000 calves. In ...

AI Technical Summary

Problems solved by technology

At present, my country's animal import and export inspection and quarantine departments mainly use neutralization tests to detect Akabane disease virus antibody in imported animal serum, which is time-consuming and laborious, and is greatly affected by other factors. The repeatability is not good, and it is not conducive to high-throughput detection.