Foot-and-mouth disease virus (FMDV) resistant monoclonal antibody and identified epitope and application thereof

Abstract

The invention discloses a foot-and-mouth disease virus (FMDV) resistant monoclonal antibody and an identified epitope and application thereof, and belongs to the field of prevention and control of the FMDV. The microbial collection number of a hybridoma cell line, which can secrete the neutralizing monoclonal antibody resisting to Asia-1 FMDV, is CGMCC No.2692; and the microbial collection number of the hybridoma cell line, which can secrete the neutralizing monoclonal antibody resisting to O-type FMDV, is CGMCC No.2691. The invention also discloses amino acid sequences of a conformational neutralizing epitope of the Asia-1 FMDV VP1 protein and a linear neutralizing epitope of the O-type FMDV VP1 protein which are identified by the two monoclonal antibodies respectively. In-vitro neutralization tests and in-vivo animal protection tests show that both the two monoclonal antibodies have excellent passive immunity effect, can be applied to emergency prevention of the FMDV and have excellent immunity effect on the passive immunity of the FMDV.

Description

technical field [0001] The present invention relates to monoclonal antibodies, in particular to neutralizing monoclonal antibodies against Asian type 1 FMDV and neutralizing monoclonal antibodies against O-type FMDV, and the present invention also relates to neutralizing epitopes recognized by the above-mentioned monoclonal antibodies, The present invention further relates to the application of the monoclonal antibody and the neutralizing epitope in preventing foot-and-mouth disease, belonging to the field of prevention and treatment of foot-and-mouth disease. Background technique [0002] Foot and mouth disease virus (Foot and mouth disease virus, FMDV) is a member of the genus FMDV in the picornaviridae family. It is a single-stranded positive-sense RNA, and its genome RNA is about 8.5 kb in length. Foot-and-mouth disease is one of the important infectious diseases that seriously harms cloven-hoofed animals. The disease causes the death of young animals, decreased milk pro...

AI Technical Summary

Problems solved by technology

These foreign studies use monoclonal antibody pressure screening or synthetic peptide scanning technology, which can only determine the amino acids or peptides related to the epitope, but cannot accurately determine the nature and position of the epitope

In my country, articles on neutralizing monoclonal antibodies to Asia-type and O-type FMDV have been published, but no research reports on neutralizing epitopes have been seen. Therefore, the functionality and value of these published neutralizing monoclonal antibodies are still unclear

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