Method for preparing porcine circovirus type 2 colloidal gold antibody fast test strip
A porcine circovirus and detection test paper technology, applied in the field of veterinary biology, can solve the problems of high cost, high detection cost, poor stability and the like
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[0074] 2.5 Preparation of expressed protein anti-mouse serum and IFA results
[0075] After immunizing the mice with the expressed protein for 3 times, the blood was collected from the sinus under the frame, and the serum was analyzed. Diluted with PBS 1:20, and infected PK15 cells with PCV2JXL for IFA, the cytoplasm and membrane-specific fluorescence could be seen in the dark field, in the form of hollow circles, and occasionally appeared in the nucleus. IFA was done with virus-inoculated cells and 1:20 diluted normal BALB / c mouse serum, or PBS was used instead of the primary antibody to interact with normal cells, no fluorescence appeared, indicating that the anti-serum contained anti-virus specific antibodies. See Figure 6
[0076] 2.6 ELISA reactivity of recombinant expressed protein with PCV2 infected pig serum
[0077] pGEX-PCV 421-37 and pPRO-Rep recombinant bacterial protein after ultrasonic disruption, coated with microtiter plates, and positive serum and negativ...
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[0082] The porcine circovirus type 2 colloidal gold antibody rapid detection test strip of the present invention has a structure consisting of a water-absorbing pad 1, a nitrocellulose membrane 2, a glass fiber membrane 3 containing a colloidal gold-labeled antigen, a gold-labeled antigen protective film 4, and a reaction The support is composed of a PVC plate 5, wherein the nitrocellulose membrane 2 is set in the middle of the reaction support PVC plate 5, and the nitrocellulose membrane 2 is provided with two color development areas T and C, wherein the T area is coated with genetically engineered pigs Pseudorabies expresses gE protein; the C area is coated with rabbit anti-swine pseudorabies IgG, on the upper surface of the left end of the nitrocellulose membrane 2T area, a glass fiber membrane 3 containing colloidal gold-labeled antigen is arranged, and the glass fiber membrane 3 containing colloidal gold-labeled antigen The upper surface of the fiber membrane 3 is provided...
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