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Immunity-chropadography test paper for detecting botulinum toxin and preparation method thereof

An immunochromatographic test paper and botulinum toxin technology, applied in the field of biochemistry, can solve the problems of time-consuming, cumbersome preparation methods, and high detection costs, and achieve the effects of wide application range, high sensitivity, and strong specificity

Inactive Publication Date: 2010-03-24
SHANGHAI HAITAI JINXIN BIOMOLECULAR DETECTION TECH
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide an immunochromatographic test paper for detecting botulinum toxin and a preparation method thereof, which is cumbersome and time-consuming time, high cost of detection technical problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Embodiment 1 detects the preparation of the immunochromatographic test paper of botulinum toxin

[0008] Material

[0009] Chlorauric acid (HAuCl 4 .4H 2 O), analytically pure, Shanghai Reagent No. 1 Factory;

[0010] Trisodium citrate (Na 3 C 6 h 5 o 7 .2H 2 O), analytically pure, Beijing Chemical Plant;

[0011] Type A toxin, Lanzhou Institute of Biological Products, 11OU / bottle;

[0012] Bovine serum albumin (BSA), analytically pure, imported repackaged;

[0013] Polyethylene glycol (PEG), analytically pure, imported sub-packaging;

[0014] Nitrocellulose membrane, purchased from Millipore, USA, HF B 504;

[0015] Glass fiber membrane, purchased from Nanjing Sunway Industrial Company;

[0016] 1) Preparation of anti-botulinum antibody

[0017] Rabbits were injected subcutaneously at multiple points (2ml / only); toxin 0.25mg+Freund's complete adjuvant immunization, rabbit epidemic was added 10 days later, a total of 2 additional times, and blood was drawn ...

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Abstract

The invention provides an immunity-chropadography test paper for detecting botulinum toxin, comprising a layer of cellulose nitrate film, wherein the anti-botulinum toxin antibody solution is sprayedon the cellulose nitrate film and the cellulose nitrate film is arranged on a water absorption pad and a gold-label pad is arranged on the cellulose nitrate film and the gold-label is glass fiber filmor resin and immune colloidal gold probe solution for resisting botulinum toxin antibody is arranged in the gold-label pad and and a sample pad is arranged on the gold-label pad. The invention also provides a preparation method of the immunity-chropadography test paper for detecting botulinum toxin. Monoclonal antibody is used and the botulinum toxin is detected using immune response principle. The sensitivity is high and the specificity is strong and the conventional animal death-causing and neutralization test detection method is substituted and the immune colloidal gold immuno-chropadographic technology is used. The invention has features of short detection period, wide application range, simple sample processing, simple use operation and low cost.

Description

Technical field: [0001] The invention relates to the field of biochemistry, in particular to an immunochromatographic test paper, in particular to an immunochromatographic test paper for detecting botulinum toxin and a preparation method thereof. Background technique: [0002] Botulinum toxin is an exotoxin produced by the bacterium botulinum and is one of the main causes of food poisoning. Laboratory detection and stereotyping of botulinum toxin are of great significance for diagnosis and treatment, and the most important thing to prevent poisoning is to strengthen monitoring. So far the classic mouse lethal and neutralization test is still the most commonly used detection method for botulinum toxin. However, this method is cumbersome and time-consuming, and the detection cost is expensive, so it is not easy to promote. Invention content: [0003] The technical problem to be solved by the present invention is to provide an immunochromatographic test paper for detecting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577
Inventor 葛海鹏
Owner SHANGHAI HAITAI JINXIN BIOMOLECULAR DETECTION TECH
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