Feline panleukopenia virus antibody ELISA kit and detection method for feline panleukopenia virus antibody ELISA

A feline parvovirus and detection method technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as highlighted safety hazards, difficult reproduction and disease purification, slow progress in animalization, etc., and achieve high sensitivity results

Inactive Publication Date: 2019-02-01
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Experimental cats are often used in physiological and pathological experiments of cranial nerves, and are also commonly used animals in pharmacological experiments and animal disease models. However, due to difficulties in feed, feeding, breeding, and disease purification, the progress of experimental animalization of cats slow
Many foreign countries have established breeding bases for experimental cats. Some countries have bred SPF cats and sterile cats through epidemic purification. However, there is no authoritative cat breeding and breeding base in China. Most of the cats used in experiments are from For pet cats or captured wild cats, the safety hazard is prominent

Method used

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  • Feline panleukopenia virus antibody ELISA kit and detection method for feline panleukopenia virus antibody ELISA
  • Feline panleukopenia virus antibody ELISA kit and detection method for feline panleukopenia virus antibody ELISA
  • Feline panleukopenia virus antibody ELISA kit and detection method for feline panleukopenia virus antibody ELISA

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1 of the present invention provides a feline parvovirus antibody ELISA kit, which includes an enzyme-labeled plate coated with an antigen and an enzyme-labeled antibody, the antigen is a purified FPV antigen, and the enzyme-labeled antibody is horseradish Oxidase labeled goat anti-cat IgG antibody.

Embodiment 2

[0032] Example 2 of the present invention provides a feline parvovirus antibody ELISA kit. The kit includes an enzyme-labeled plate coated with an antigen and an enzyme-labeled antibody. The antigen is a purified FPV antigen, and the enzyme-labeled antibody is horseradish. The peroxidase-labeled goat anti-cat IgG antibody, the working concentration of FPV purified antigen is 5 μg / mL, and the dilution factor of the enzyme-labeled antibody is 1:6000.

Embodiment 3

[0034] Example 3 of the present invention provides a feline parvovirus antibody ELISA kit. The kit includes an enzyme-labeled plate coated with an antigen, an enzyme-labeled antibody, a blocking solution and a rinse solution. The antigen is a purified FPV antigen and the enzyme The labeled antibody is goat anti-cat IgG antibody labeled with horseradish peroxidase, the working concentration of FPV purified antigen is 5μg / mL, the dilution factor of the enzyme-labeled antibody is 1:6000, and the blocking solution contains 5% degreasing The PBS buffer solution of milk, and the rinsing solution is a PBS buffer solution containing 0.05% Tween-20.

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Abstract

The invention provides a feline panleukopenia virus antibody ELISA kit and a detection method for a feline panleukopenia virus antibody ELISA. The feline panleukopenia virus antibody ELISA kit comprises an enzyme-labeled plate and an enzyme-labeled antibody which are coated with antigens; the antigens are FPV purified antigens, and the enzyme-labeled antibody is a goat anti-feline IgG antibody labeled by horse radish peroxidase; and the feline panleukopenia virus antibody ELISA kit and the detection method for the feline panleukopenia virus antibody ELISA have very high sensibility, specificity and accuracy for the detection of the feline panleukopenia virus antibody.

Description

Technical field [0001] The invention belongs to the field of feline parvovirus antibody detection, and particularly relates to an ELISA kit for feline parvovirus antibody. Background technique [0002] Experimental cats are often used in physiology and pathology experiments of brain nerves, and are also commonly used animals in pharmacological experiments and animal disease models. However, due to difficulties in feed, breeding, reproduction, and disease purification, the experimental animalization of cats is progressing slow. Many foreign countries have established breeding bases for experimental cats. Some countries have bred SPF cats and sterile cats through disease purification. However, there is currently no authoritative cat breeding and breeding bases in China. Most of the cats used for experiments are from For pet cats or captured wild cats, safety hazards are highlighted. [0003] Feline panleukopenia virus (FPV), also known as feline panleukopenia syndrome virus, feline...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/569
CPCG01N33/535G01N33/56983
Inventor 陆涛峰陈洪岩赵丽丽张圆圆
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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