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Triple egg yolk antibody for cats, preparation, preparation method and application

A yolk antibody and antibody titer technology, applied in the direction of antibodies, antiviral agents, antibody medical components, etc., can solve the problems of poor treatment effect and treatment specificity, drug resistance and side effects, and achieve shortened treatment time, low cost, The effect of good treatment

Active Publication Date: 2022-06-17
北京华驰千盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is to overcome the poor therapeutic effect and specificity of treatment in the existing treatment methods for feline distemper, feline nasal branch and feline calicivirus infection, and the problems of drug resistance and toxic side effects, Thereby providing a triple yolk antibody for cats, preparation, preparation method and use

Method used

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  • Triple egg yolk antibody for cats, preparation, preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment provides a preparation method of triple yolk antibody for cats, comprising the following steps:

[0052] (1) Preparation of triple inactivated vaccine

[0053] Use F-81 cells to culture feline parvovirus (FPV), feline herpesvirus (FHV) and feline calicivirus (FCV) respectively (the culture medium is DMEM medium), and cultivate until more than 80% of the F-81 cells After CPE (cytopathic effect), the culture was repeatedly frozen and thawed 3 times, and the cultured virus liquid was collected by centrifugation at 3000r / min, and the TCID of the virus in each virus liquid was measured. 50 . After determination, the virus content of FPV in the feline parvovirus fluid was 1×10 6.5 TCID 50 / 0.1ml, the virus content of FHV in the cat herpes virus liquid is 1×10 7.0 TCID 50 / 0.1ml, the virus content of FCV in cat calicivirus solution is 1×10 6.5 TCID 50 / 0.1ml.

[0054] Concentrate the collected three kinds of serum-free virus liquids by 2 times respectiv...

Embodiment 2

[0063] Prepare the triple yolk antibody for cats according to the method of Example 1, the difference is: in this embodiment, the first immunization dose is 1ml / cat, the second immunization dose is 2ml / cat, and the third immunization dose is 2ml / cat Only.

[0064] In this embodiment, the immunized eggs on the 12th to 150th days after the third immunization were collected. After 150 days, the titer of each yolk antibody in the eggs was lower than the collection standard, so the collection was stopped. During the collection period, the egg production rate of laying hens averaged was 79.1%. The titer of the cat triple yolk antibody prepared in this example was determined, wherein the neutralizing antibody titer of the feline parvovirus yolk antibody was 1:1024, and the neutralizing antibody titer of the feline herpesvirus yolk antibody was 1:128. The neutralizing antibody titer of feline calicivirus yolk antibody was 1:128.

Embodiment 3

[0066]Prepare the triple yolk antibody for cats according to the method of Example 1, the difference is: in this embodiment, the first immunization dose is 1.5ml / , the second immunization dose is 1.5ml / , and the third immunization dose is 2.5ml / piece.

[0067] In this example, the immunized eggs were collected on the 10th to 150th day after the third immunization. After 150 days, the titer of each yolk antibody in the eggs was lower than the collection standard, so the collection was stopped. During the collection period, the egg production rate of laying hens averaged was 79.2%. The titer of the cat triple yolk antibody prepared in this example was determined, wherein the neutralizing antibody titer of the feline parvovirus yolk antibody was 1:1024, and the neutralizing antibody titer of the feline herpesvirus yolk antibody was 1:128. The neutralizing antibody titer of feline calicivirus yolk antibody was 1:128.

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Abstract

The invention relates to the technical field of biology, in particular to a triple egg yolk antibody for cats, a preparation, a preparation method and application. Wherein the triple egg yolk antibody comprises a cat parvovirus egg yolk antibody, a cat herpes virus egg yolk antibody and a cat calicivirus egg yolk antibody. The triple egg yolk antibody provided by the invention can be used for prevention and adjuvant treatment of feline plague, feline nasal branch and feline infectious nasal conjunctivitis, and is high in protection rate and good in effect. Particularly, the traditional Chinese medicine composition has a good treatment effect or an auxiliary treatment effect on the feline plague diarrhea, and the treatment time of the feline plague diarrhea can be remarkably shortened. According to the invention, the treatment approach of the feline plague diarrhea is expanded, and a new direction is provided for the treatment of the feline plague diarrhea.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a triple yolk antibody for cats, a preparation, a preparation method and an application. Background technique [0002] Feline distemper, also known as feline panleukopenia and feline infectious enteritis, is an acute highly contagious infectious disease caused by feline parvovirus (Felineparvovirus, FPV). Dehydration, circulatory disturbance and sharp reduction of white blood cells are the main features. The disease is highly contagious, and it is more contagious in incompletely vaccinated or unvaccinated cats, and it mostly infects young cats aged 3 to 5 months. If a female cat is infected with feline distemper during pregnancy, it will cause problems such as stillbirth, miscarriage, and neurological symptoms in newborn kittens. In addition to infecting domestic cats, FPV can also infect other cats (such as tigers and leopards), mustelids (such as mink) and bears (such as raccoons...

Claims

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Application Information

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IPC IPC(8): C07K16/08C07K16/10C07K16/02A61K39/42A61P31/20A61P31/22A61P31/14
CPCC07K16/081C07K16/085C07K16/10C07K16/02A61P31/20A61P31/22A61P31/14C07K2317/11C07K2317/76A61K2039/507A61K2039/5252A61K2039/552A61K2039/70
Inventor 刘巧荣张谦
Owner 北京华驰千盛生物科技有限公司
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