The invention belongs to the technical field of biology, and particularly relates to an IgG1Fc monomer and a preparation method and application thereof. In accordance with the sequence of a novel IgG1Fc monomer reformed in an antibody IgG1 constant region, through an antibody engineering technique, a human antibody IgG1 constant region Fc is reformed, so that an Fc dipolymer becomes an Fc monomer,an FcRn combination function is maintained, and an extremely low irrelevant protein non-specific combination characteristic is achieved; main features of the Fc monomer lie in that the Fc monomer hasmutation at positions of T366, L368, P395 and K409 in a CH3 region of the antibody constant region, has high-efficiency expression in prokaryotic cells, can use combination of a pH dependent specialcombination mode with FcRn, and has extremely low nonspecific combination characteristics. Through the novel Fc monomer, the IgG1Fc monomer can fuse or couple with various protein, polypeptide, micromolecules, nucleic acid and the like for different target points, so that fused or coupled molecules have the characteristic of combining pH dependence with FcRn.