Building method and mass detection method for fingerprint of total alkaloid components of cortex mori radicis medicinal material or cortex mori radicis extract

A technique for extracting and constructing method of Morus alba radix, applied in the directions of material separation, measuring device, analyzing material, etc., can solve the problem that the total alkaloid active component content and single component of Morus alba radix cannot be fully reflected, and improve the quality Control standard, good stability and good reproducibility

Active Publication Date: 2013-01-23
BEIJING WBL PEKING UNIV BIOTECH
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  • Claims
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Problems solved by technology

[0007] In order to solve the problem that only a single component can be detected in the existing quality inspection process of Cortex Morus alba or the extract of Cortex Morus alba, and the content of the total alkaloid active ingredients in Cortex Morus alba cannot be fully reflected, the present invention provides a cortex Cortex Morus alba Method for constructing fingerprints of total alkaloid components in medicinal materials or Morus alba extracts

Method used

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  • Building method and mass detection method for fingerprint of total alkaloid components of cortex mori radicis medicinal material or cortex mori radicis extract
  • Building method and mass detection method for fingerprint of total alkaloid components of cortex mori radicis medicinal material or cortex mori radicis extract
  • Building method and mass detection method for fingerprint of total alkaloid components of cortex mori radicis medicinal material or cortex mori radicis extract

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Experimental program
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Effect test

preparation example Construction

[0028] (a) Preparation of Cortex Morus alba Medicinal Material Solution: With 0.02mol / L hydrochloric acid solution or water as solvent, the powder of Cortex Morus alba medicinal material is formulated into Cortex Morus alba medicinal material solution, preferably adopting 0.02mol / L hydrochloric acid solution as solvent , because the alkaloid components are generally weakly alkaline, the salt-forming effect in the extraction process with dilute hydrochloric acid can ensure the maximum extraction rate of the alkaloid components. Of course, the purpose of the present invention is to prepare the Cortex Mori medicinal material into a solution that can be used for high-performance liquid chromatography separation, so under this purpose, the solvent used in the present invention is not limited to 0.02mol / L hydrochloric acid solution or water. The concentration of the solution can be determined according to the loading capacity of the chromatographic column used, the flow rate of the m...

Embodiment 1

[0039] Example 1 Establishment of Control Fingerprint of Cortex Morus alba Medicinal Material

[0040] (1) The source of Morus alba medicinal material:

[0041] The 20 batches of Morus alba medicinal materials used in the embodiments of the present invention are mainly produced in Henan, Hebei, Anhui, Guangxi, and Hunan. The above-mentioned varieties have been identified and meet the pharmacopoeia standards without obvious biological differences. See Table 1 for details.

[0042] Table 1

[0043] batch number place of origin Are there biological differences Y070609HN Henan none Y100513HB Hebei none Y100716HB Hebei none Y101113HB Hebei none Y101229HB Hebei none Y110104HN Hunan none Y101230AH Anhui none Y100826HN Henan none

[0044] Y101026HB Hebei none Y101102HN Henan none Y110224GZ Guangxi none Y110225HN Henan none Y110226HN Hunan none ...

Embodiment 2

[0053] Example 2 Method for detecting the quality of fingerprints of Morus alba medicinal material

[0054] (1) Preparation of the Morus alba medicinal material solution to be tested: pass the Morus alba medicinal material (batch number Y100513HB) powder through a No. 3 sieve, accurately weigh 0.2g, put it in a 250ml volumetric flask, and add an appropriate amount of 0.02mol / L hydrochloric acid solution , sonicate (power 250W, frequency 28KHZ) for 45 minutes, let cool, add 0.02mol / L hydrochloric acid solution to the mark, shake well, and let stand;

[0055] (2) Preparation of reference substance solution: accurately weigh an appropriate amount of 1-deoxynojirimycin hydrochloride reference substance, add water to make a solution containing 4 μg per 1 ml, and obtain it;

[0056] (3) according to the chromatographic determination method of embodiment 1 and the fingerprint spectrum of measuring the cortex Mori medicinal material to be tested, as Figure 4 shown;

[0057] (4) The...

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Abstract

The invention provides a building method and a mass detection method for a fingerprint of the total alkaloid components of a cortex mori radicis medicinal material or a cortex mori radicis extract. The building method comprises the following steps of: (1) preparing a cortex mori radicis medicinal material solution or a cortex mori radicis extract solution; and (b) using a high performance liquid chromatography to detect either the fingerprint of the total alkaloid components of the cortex mori radicis medicinal material or the fingerprint of the total alkaloid components of the cortex mori radicis extract. Either the fingerprint of the total alkaloid components of the cortex mori radicis medicinal material or the fingerprint of the total alkaloid components of the cortex mori radicis extract is obtained by the building process, thereby comprehensively reflecting the total alkaloid active components in the cortex mori radicis medicinal material. Therefore, the mass control standards of the cortex mori radicis medicinal material and the cortex mori radicis extract are improved, and the building method of the fingerprint provided by the invention has the advantages of good stability and good reproducibility. The mass problem of the existing cortex mori radicis medicinal material can be accurately adjusted in the market by comparing with the fingerprints.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicinal materials, in particular to a fingerprint construction method and a detection method for total alkaloid components in Morus alba medicinal material or Morus alba extract. Background technique [0002] Morus alba L. is the dried root bark of Morus alba L., a plant of the Moraceae family. It was first recorded in "Shen Nong's Materia Medica", listed as a middle grade, and has been recorded in successive dynasties of herbal medicines. Cortex Mori is sweet and cold in nature, and belongs to the lung meridian. It has the functions of purging lung, relieving asthma, diuresis and reducing swelling. The main chemical components of Morus alba include: flavonoids, piperidine alkaloids (represented by 1-deoxynojirimycin), coumarins, polysaccharides, etc. Morus alba extract is prepared from Morus alba medicinal material: first cut or pulverize the Morus alba medicinal materia...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36
Inventor 段震文郭树仁薛岚
Owner BEIJING WBL PEKING UNIV BIOTECH
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