Method for measuring content of tannide in nakedflower beautyberry

A kind of naked flower purple beads, the technology of the determination method, applied in the direction of pharmaceutical formulation, color/spectral property measurement, medical preparations containing active ingredients, etc., to achieve the effect of improving quality control standards

Active Publication Date: 2010-01-13
HAINAN JIUZHITANG PHARMACY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are nudehuazizhu tablets, nudehuazizhu capsules, nudehuazizhu suppositories and other preparations, but the content in the quality standard is only controlled by total flavonoids

Method used

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  • Method for measuring content of tannide in nakedflower beautyberry
  • Method for measuring content of tannide in nakedflower beautyberry
  • Method for measuring content of tannide in nakedflower beautyberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1), the content assay method of tannins in the naked flower purple pearl extract

[0031] Instruments, Reagents and Controls

[0032] Instruments: TU-1901 double-beam ultraviolet-visible spectrophotometer (Beijing Puxi General Instrument Co., Ltd.); D2F-6030A vacuum drying oven (Shanghai Yiheng Scientific Instrument Co., Ltd.).

[0033] Reagents: phosphomolybdotungstic acid test solution (self-made), methanol, sodium carbonate (analytical pure), casein (chemical pure).

[0034] Reference substance: gallic acid (99.9% pure).

[0035] Preparation of Auranthus nudiflora extract:

[0036] Take 100kg of naked flower purple pearl herbs, add 1500kg of water, soak for 30 minutes, heat and boil for 30 minutes, pour out the decoction, decoct the medicinal residues twice, add 1500kg of water each time, boil for 60 minutes, combine the decoction for three times, filter, and pass the filtrate The specific surface area is 350~450m 2 JD-1 (WLD) type polystyrene macroporous adsorp...

Embodiment 2

[0062] The preparation of the extract of Zizhu nudiflora: Take 100kg of Zizhu nudiflora medicinal material, add 1000kg of water, soak for 50 minutes, heat and boil for 90 minutes, pour out the decoction, decoct the dregs for 2 times, add 1500kg of water each time, boil for 90 minutes, Combine the three decoctions, filter, concentrate the filtrate under reduced pressure to a relative density of 1.1, freeze-dry, test and subpackage to obtain the extract of Aurora nudiflora. The yield of P. nudiflora extract was 9.7%.

[0063] Preparation of reference substance solution: Take an appropriate amount of gallic acid reference substance, weigh it accurately, add water to make a solution containing 0.0541 mg of gallic acid per 1 ml, and use it as the reference substance solution.

[0064] Preparation of the sample solution: take about 0.7g of the extract of Auranthus nudica, put it in a 250ml volumetric flask, add 150ml of water to extract overnight, add water to the mark after ultraso...

Embodiment 3

[0070] The preparation of the extract of Zizhu nudiflora: Take 100kg of Zizhu nudiflora medicinal material, add dilute alcohol 1000kg, soak for 50 minutes, heat and boil for 90 minutes, pour out the decoction, decoct the medicinal residue twice, add water 1500kg each time, boil for 90 minutes Minutes, combined three decoctions, filtered, the filtrate passed through a specific surface area of ​​350 ~ 450m 2 / g, skeleton density of 1-1.2g / ml, average pore diameter of 35-65nm, porosity of 60-68% D101 polystyrene macroporous adsorption resin column, diameter-to-height ratio of the resin column is 1:7 , discard the effluent, wash the column body with 4 times the column volume of water, discard the washing solution, and elute with 50% ethanol until no flavonoids are detected in the eluent. Collect the eluate, recover the ethanol, concentrate under reduced pressure to a relative density of 1.1, freeze-dry, test and subpackage to obtain the extract of Aurora nudiflora. The yield of t...

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Abstract

The invention relates to a method for measuring the content of tannide in nakedflower beautyberry. The invention adopts a light splitting photometric method to measure the content of the tannide in the nakedflower beautyberry, further enhances the controllability of nakedflower beautyberry medicinal materials and preparations and ensures the quality of nakedflower beautyberry extracts.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine. More specifically, it relates to a method for determining the content of tannins in the nudibranch or in the nudibranch extract. Background technique [0002] Callicarpa nudiflora Hook.et Arn. is the dry leaf of the Verbenaceae plant Callicarpa nudiflora Hook.et Arn. It mainly has the effects of astringent and hemostasis, clearing away heat and detoxifying, and can be used for various internal and external bleeding diseases, especially for lung and stomach bleeding. It is multi-purpose, and can also treat burns, heat, poisonous sores and cancer. At present, there are nudihuazizhu tablets, nudihuazizhu capsules, nudihuazizhu suppositories and other preparations, but the content in the quality standard is only controlled by total flavonoids. [0003] Tannins, also known as tannins, are a class of complex polyphenolic compounds present in plants. Modern studies have shown tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31A61K36/86A61P7/04A61K127/00
Inventor 宁云山关继峰严冬兰
Owner HAINAN JIUZHITANG PHARMACY
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