Industrialization preparation method of mogroside V

A technology of mogroside and mogroside, applied in the field of industrialized extraction and preparation of mogroside V, can solve the problems of complicated process and low purity, and achieve the effects of improving safety, cheap solvent and saving cost.

Inactive Publication Date: 2015-04-22
黄晓 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the cumbersome process and low purity existing in the traditional process of refining the crude product of mogroside V, the purpose of the present invention is to provide an industrialized preparation method of mogroside V, which simplifies the preparation method and has the characteristics of on-line separation

Method used

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  • Industrialization preparation method of mogroside V

Examples

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Effect test

Embodiment 3

[0029] Example 3——This example uses 22 μm n-octadecyl reverse-phase silica particles, and the rest of the process is the same as in Example 2, and the purity of mogroside V obtained is 97.0%.

Embodiment 4

[0030] Example 4—The mobile phase is methanol and water, the gradient range is 2:8 to 4:6, the control flow rate is 100ml / min, and the elution time is 50min; after that, the gradient of the above-mentioned organic solvent aqueous solution is adjusted to 9:1, The elution was continued until 60 min, and the rest of the process was the same as in Example 2, and the purity of the obtained mogroside V was 97.5%.

Embodiment 5

[0031] Example 5—The mobile phase is acetonitrile and water, the gradient range is 2:8 to 3:7, the flow rate is controlled at 100ml / min, and the elution time is 30min; after that, the gradient of the above-mentioned organic solvent aqueous solution is adjusted to 5:5, The elution was continued until 60 min, and the remaining processes were the same as in Example 2, and the purity of the obtained mogroside V was 98.1%.

[0032] It can be seen from the introductions of the above examples that, through the preparation method of the present invention, the crude mogroside V after crude extraction can be directly used to prepare mogroside V without a large number of complicated and tedious pre-treatment and post-treatment processes, and the solvent used is cheap, safe and easy to separate The purification process can be monitored online in real time, which greatly improves safety and saves costs, and is suitable for large-scale industrial production. The final measurement by high pe...

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Abstract

The invention discloses an industrialization preparation method of mogroside V. The industrialization preparation method comprises the following steps: I, preparing a crude mogroside product by using a digestion and dehydration method; II, preparing the crude mogroside product into a solution by using water, and filtering off undissolved substances; III, pumping the mogroside solution into a dynamic axial compression column preparative chromatographic system, wherein the column is filled with reversal phase special silica gel as packing, the feeding amount of the packing is 0.1-3%, and an organic solvent solution is adopted as the flowing phase in the column; firstly, eluting for 30-50 minutes when controlling the initial gradient of the organic solution to (2:8) to (4:6) and the flow speed to be 100ml/minute, further eluting to 50-60 minutes when adjusting the gradient to be greater than or equal to 1, adjusting the detection wavelength of an ultraviolet luminosity detector to be 203nm, injecting the flow phase into the dynamic axial compression column preparative chromatographic system for liquid chromatogram detection, and collecting eluant corresponding to a chromatographic peak on line, thereby obtaining mogroside V of which the purity is greater than 95%. By adopting the industrialization preparation method disclosed by the invention, a treatment process is simplified, on-line real-time detection is achieved, the security is improved, the cost is saved, and ideal purity of mogroside V is achieved.

Description

technical field [0001] The invention relates to the field of extracting effective components of Luo Han Guo fruit, in particular to an industrial extraction and preparation technology of mogroside V. Background technique [0002] Luo Han Guo [Siraitia grosuenorii] is the fruit of the Cucurbitaceae plant Luo Han Guo. It is a natural product resource with great potential for development. Mogroside is the main active ingredient in Luo Han Guo. It is a triterpene glucoside with a wide range of biological properties. . Among them, mogroside V is a component with high content and sweetness in Luo Han Guo fruit, its content is about 1%, and its sweetness is equivalent to 300 times that of sucrose, and it is the main sweet component. Mogroside has the characteristics of safety, good taste, high sweetness, good thermal stability, light color, and little influence by the pH of the external environment (pH value is between 2 and 10). my country officially approved the product as a fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 黄晓黄曦
Owner 黄晓
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