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Method for simultaneously extracting Mogroside V and VI and 11-O-based glucoside V

A mogroside and base glycoside technology, applied in chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problem of obtaining high-purity glycoside products, separation and purification of Luo Han Guo 11-O base glycoside V and glycoside VI There are few reports and other problems, to achieve the effect of improving utilization, increasing added value, and mild conditions

Active Publication Date: 2017-07-21
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

However, the mogroside obtained by this method is also a mixture of various sweet glycosides, and high-purity products of each sweet glycoside have not been obtained.
[0012] It can be seen from the above that there are few reports on the separation and purification of the 11-O-based glycoside V and glycoside VI of Luo Han Guo

Method used

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  • Method for simultaneously extracting Mogroside V and VI and 11-O-based glucoside V

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037](1) n-butanol extraction: Dissolve 100g of the refined mother liquor of mogroside V in 500g of water, add 500g of n-butanol for extraction, separate the water layer to obtain the n-butanol layer;

[0038] (2) Concentration and crystallization: Concentrate the n-butanol layer obtained in step (1) under reduced pressure at 80°C and a vacuum of -0.07MPa until the mass concentration of the concentrated solution is 25%, cool it at 15°C, and rotate at 120r / min, stirred and crystallized for 12h, filtered to obtain crystals and n-butanol crystallization mother liquor, dried the crystals to obtain 18.2g mogroside VI;

[0039] (3) Polyamide chromatography: Concentrate the n-butanol crystallization mother liquor obtained in step (2) at 80°C and a vacuum of -0.07MPa to no alcohol under reduced pressure, and dissolve 70g of the obtained concentrate in 1750g of water. The flow rate of 1BV / h passes through the polyamide chromatography column (among them, the amount of polyamide used i...

Embodiment 2

[0043] (1) n-butanol extraction: Dissolve 200g of the refined mother liquor of mogroside V in 1600g of water, add 2400g of n-butanol for extraction, separate the water layer to obtain the n-butanol layer;

[0044] (2) Concentration and crystallization: Concentrate the n-butanol layer obtained in step (1) under reduced pressure at 85°C and a vacuum of -0.08MPa until the mass concentration of the concentrated solution is 30%, cool it at 30°C, and rotate at 80r / min, stirred and crystallized for 36h, filtered to obtain crystals and n-butanol crystallization mother liquor, dried the crystals to obtain 34.3g mogroside VI;

[0045] (3) Polyamide chromatography: The n-butanol crystallization mother liquor obtained in step (2) was concentrated under reduced pressure to no alcohol at 85°C and a vacuum of -0.08MPa, and 135g of the obtained concentrate was dissolved in 2700g of water, and then Pass through the polyamide chromatography column at a flow rate of 0.5BV / h (the amount of polya...

Embodiment 3

[0049] (1) n-butanol extraction: Dissolve 150g of the refined mother liquor of mogroside V in 900g of water, add 900g of n-butanol for extraction, separate the water layer to obtain the n-butanol layer;

[0050] (2) Concentration and crystallization: Concentrate the n-butanol layer obtained in step (1) under reduced pressure at 75°C and a vacuum of -0.06MPa until the mass concentration of the concentrated solution is 30%, cool it at 25°C, and rotate at a speed of 60r Stir and crystallize for 24 hours at a temperature of 1 / min, filter to obtain crystals and n-butanol crystallization mother liquor, dry the crystals to obtain 25.8g mogroside VI;

[0051] (3) Polyamide chromatography: The n-butanol crystallization mother liquor obtained in step (2) was concentrated under reduced pressure to no alcohol at 75°C and a vacuum degree of -0.06MPa, and 100g of the obtained concentrate was dissolved in 2000g of water to Pass through the polyamide chromatography column at a flow rate of 1....

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Abstract

The invention discloses a method for simultaneously extracting Mogroside V and VI and 11-O-based glucoside V. The method comprises the following steps: (1) dissolving Mogroside V refining mother solution, extracting by n-butyl alcohol to obtain a n-butyl alcohol layer; (2) concentrating, cooling, crystallizing, and filtering to obtain a crystal and crystallization mother solution, and drying the crystal to obtain the Mogroside VI; (3) concentrating the crystallization mother solution until the alcohol is not existent, dissolving, and passing through a polyamide chromatography column, collecting effluent, concentrating, and drying to obtain the Mogroside V; (4) eluting the chromatography column by using the low-concentration alcohol and the high-concentration alcohol, collecting high-concentration alcohol eluant, concentrating, cooling, crystallizing, filtering, and drying to obtain Momordica grosvenori 11-O-based glucoside V. The purity of the Mogroside VI obtained by the method is greater than or equal to 97%, and the yield is greater than or equal to 83%; the purity of the Mogroside V is greater than or equal to 89%, and the yield is greater than or equal to 89%; and the purity of the 11-O-based glucoside V is greater than or equal to 96%, and the yield is greater than or equal to 85%. The condition of the method is moderate, safe and environmental, and the method is suitable for the industrial production.

Description

technical field [0001] The invention relates to a method for extracting mogroside, in particular to a method for simultaneously extracting mogroside V, VI and 11-O group glycoside V. Background technique [0002] Luo Han Guo is a traditional medicinal and edible plant in my country. Mogroside is its main functional component. It is an ideal natural sweetener with high sweetness and low calories. It has attracted people's attention because of its various biological activities. . There are many kinds of mogrosides. Studies have shown that mogrosides are a kind of triterpene glucoside, and its aglycone is a triterpene alcohol. There are two glucoside side chains composed of four or less glucose units. -Glycosidic bonds are connected to aglycones, and the connecting bonds between side chain glucose and sugar are β-1,6 and β-1,2 glycosidic bonds. Among them, mogroside V has the highest content, followed by 11-O-based glycoside V and glycoside VI. The molecular formulas of the th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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