Production method for high-content mogroside V

A mogroside, production method technology, applied in chemical instruments and methods, glycoside steroids, steroids and other directions, can solve the problems of large water consumption, complex process, low yield and the like, and achieve the effect of improving content and yield

Active Publication Date: 2019-02-12
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many extraction methods of mogroside V in the prior art, but they all have many defects such as complex process, low yield, low product purity, and large water consumption (such as Chinese patent application CN201410190403.7)

Method used

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  • Production method for high-content mogroside V
  • Production method for high-content mogroside V

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Pretreatment: put fresh Luo Han Guo in a low-temperature freezer at -25°C, freeze for 1 hour, and immediately heat it with steam at 105°C for 20 seconds;

[0027] 2) Extraction: Peel the pretreated Luo Han Guo, crush the pulp (the seeds remain intact), add water 2.5 times the mass of the pulp to carry out countercurrent circulation extraction, and the extraction temperature is 50°C to obtain the extract;

[0028] 3) Filtration: After the extract is centrifuged by a decanter centrifuge with a separation factor of 3000, the supernatant is filtered through a ceramic membrane with a membrane tube aperture of 50 nm to obtain the filtrate;

[0029] 4) Adsorption and analysis: After the filtrate is absorbed by the DA201-H macroporous resin column, it is pre-washed with 1BV of sodium hydroxide solution with a mass concentration of 2‰, and then pre-washed with 1BV of a hydrochloric acid solution with a mass concentration of 2‰ , then wash with 2BV of pure water until the colu...

Embodiment 2

[0033] 1) Pretreatment: put fresh Luo Han Guo in a low-temperature freezer at -10°C, freeze for 1.5 hours, and immediately heat it with steam at 120°C for 30 seconds;

[0034] 2) Extraction: Peel the pretreated Luo Han Guo, break the pulp (the seeds remain intact), add water 6 times the mass of the pulp to carry out countercurrent circulation extraction, and the extraction temperature is 90°C to obtain the extract;

[0035] 3) Filtration: the extract is centrifuged by a decanter centrifuge with a separation factor of 3500, and then centrifuged by a disc centrifuge with a separation factor of 8500. The supernatant is filtered through a ceramic membrane with a membrane tube pore size of 200 nm to obtain the filtrate;

[0036] 4) Adsorption and analysis: After the filtrate is adsorbed by T-28 macroporous resin column, it is pre-washed with 3BV of sodium hydroxide solution with a mass concentration of 6‰, and then pre-washed with 3BV of hydrochloric acid solution with a mass concen...

Embodiment 3～4

[0041] The preparation method of Examples 3-4 is similar to that of Example 2, the only difference being that the specific process parameters are different (see the table below for details, wherein the "amount of water added" is the multiple of the mass of the raw material Luo Han Guo pulp).

[0042]

[0043]

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Abstract

The invention discloses a production method for high-content mogroside V, which belongs to the field of natural plant extract production. Grosvenor momordica fruits which are immediately treated by instantaneous high-temperature heating after being frozen are adopted as a raw material. The invention has the advantages of low extraction cost, high yield, high product purity, little water consumption, simple process, capability of realizing continuous industrial mass production and the like.

Description

technical field [0001] The invention belongs to the field of natural plant extraction, and in particular relates to an industrial production method of food ingredients. Background technique [0002] Luo Han Guo (Siraitia grosvenorii) is a perennial vine of Cucurbitaceae with both medicinal and edible uses. Traditional Chinese medicine uses its fruit as medicine, which contains mogroside, various amino acids and vitamins and other medicinal ingredients. Mogroside in Luo Han Guo belongs to cucurbitane triterpene glycosides, mainly including mogroside IV, mogroside V, and simonoside I, etc. Among them, mogroside V has the highest content, accounting for about 0.4-0.5% of fresh fruit weight. It is equivalent to 300 times that of sucrose, but it has the characteristics of not inducing dental caries and not causing blood sugar to rise. It is an excellent sweetener for the general population and patients with obesity, high blood pressure and diabetes. Clinical trials have proved ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 连运河额尔敦巴雅尔蔡亚园焦利卫杨文江
Owner CHENGUANG BIOTECH GRP CO LTD
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