Method for quickly producing astaxanthin from haematococcus pluvialis stimulated by brassinosteroids

A technology of brassinolide and Haematococcus pluvialis, applied in the biological field, can solve the problems of complex technical process, high equipment requirements, difficult operation, etc., and achieve the effects of improving accumulation efficiency and low cost

Inactive Publication Date: 2011-02-16
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for efficient use of natural resources like Hemaonasia bacterium (Haemolytica sp.) which has potential benefits such as increased photosynthesis or reduced chlorophyll activity when used alone without other chemicals added.

Problems solved by technology

Technology Problem addressed in this patents relates to improving the effectiveness or safety of nitrosothricins due to their ability to enhance photosynthetic response when exposed to certain types of compounds called prostaglandins. These molecules act like sterilizing agents and reducing harmful sideways emissions caused by organisms involved in respiration processes. However, these beneficial characteristics make them challenging targets because many methods currently exist only target specific enzymes associated with astoxynthesis. Therefore, new techniques aim at developing more effective sources of nonsystem alkaline metal ion hydride crystalstals containing astegaquinone, specifically lithochloroferrite beta hemisuccinate (LHC).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Step 1. Preparation of algae liquid: Haematococcus pluvialis was selected from 712 algae strains, using MCM medium, cultured at a temperature of 19°C, a light intensity of 1500lx, and a light-to-dark ratio of 12h / 12h, and cultivated under fluorescent lamps until pluvialis red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0017] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinosteroid produced by Zhongke Ruitai (Beijing) Biotechnology Co., Ltd. was used as the brassinosteroid. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the solution was 4.95 mg / L; then th...

Embodiment 2

[0019] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultivated under the conditions of temperature 20°C, light intensity 1800lx, and light-to-dark ratio 12h / 12h, and cultivated under fluorescent lamp until Pluvialls red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0020] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinolide produced by Beijing Zhongke Ruitai Biotechnology Co., Ltd. was used as the brassinolide. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the solution was 5.0 mg / L; then the a...

Embodiment 3

[0022] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultured at a temperature of 21°C, a light intensity of 1600lx, and a light-to-dark ratio of 12h / 12h, and cultivated under a fluorescent lamp until pluvialis The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0023] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinosteroid produced by Zhongke Ruitai (Beijing) Biotechnology Co., Ltd. was used as the brassinosteroid. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the liquid was 5.05mg / L; then the above...

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Abstract

The invention provides a method for quickly producing astaxanthin from haematococcus pluvialis stimulated by brassinosteroids. The method comprises the following steps: (1) liquid preparation: culturing haematococcus pluvialis cells to an exponential phase to obtain of inductive cultured liquid; and (2) astaxanthin accumulation: dissolving the brassinosteroids with absolute ethanol of double volume, and preparing 1mg/mL brassinosteroids mother solution with dimethylsulfoxide, and adding the brassinosteroids mother solution to the cultured liquid until the final concentration of the brassinosteroids is between 4.95 and 5.05mg/L; and culturing the brassinosteroids-containing liquid under composite stress conditions of temperature of between 25 and 28 DEG C, light intensity of between 4,500 and 5,000lx, continuous lighting of fluorescent lamp and nutrient hunger until the haematococcus pluvialis cells completely turn red under observation of an optical microscope, and yield of the astaxanthin reaches maximum. The method has the advantages of simpleness and easy operation and low cost, and can greatly shorten the time that the haematococcus pluvialis cells completely turn red, so the efficiency for producing the astaxanthin from the haematococcus pluvialis cells is greatly improved.

Description

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Claims

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Application Information

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Owner SHANDONG UNIV OF TECH
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