Dendrobium officinale seed tissue culture medium

A technology of tissue culture medium and Dendrobium candidum, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of reduced nutrition and value, low nutritional content and curative effect of Dendrobium candidum, and achieve accelerated cell mitosis, The effect of accelerating the germination time and promoting growth

Inactive Publication Date: 2016-07-13
LINGYUN LONGEVITY IMMORTAL GRASS BIOTECH SCI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nowadays, more of Dendrobium candidum is used for tissue culture, but we also know that there will be a problem with this method. We continue to use the tissue of Dendrobium candidum for tissue culture, and Dendrobium candidum is continuously carried out in each tissue culture. The nutrition and value of the cultivated Dendrobium candidum will be greatly reduced through subculture and reproduction, so many people can purchase Dendrobium candidum in the market at a very low price, but its nutritional components and curative effect are relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A tissue culture medium for dendrobium officinale seeds, comprising mixing mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V and mother liquor VI according to a volume ratio of 3:1:1:1:2:0.03, and then boiling to adjust pH=5.5 , and finally after autoclaving;

[0025] Mother liquor I is composed of the following substances in parts by weight: ammonium nitrate 800mg / L, potassium dihydrogen phosphate 140mg / L, magnesium sulfate 170mg / L, potassium nitrate 900mg / L, calcium chloride dihydrate 900mg / L;

[0026] Mother liquor II is composed of the following parts by weight: copper sulfate pentahydrate 0.04mg / L, boric acid 6mg / L, manganese sulfate 16mg / L, zinc sulfate 8mg / L, cobalt chloride 0.03mg / L, potassium iodide 0.35mg / L, Sodium molybdate 0.15mg / L;

[0027] Mother liquor III is composed of the following materials in parts by weight: 25 mg / L of ferrous sulfate heptahydrate, 35 mg / L of sodium edetate;

[0028] The mother liquor IV is co...

Embodiment 2

[0032] A tissue culture medium for dendrobium officinale seeds, comprising mixing mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V and mother liquor VI according to a volume ratio of 5:2:2:2:3:0.05, and then boiling to adjust pH=6.5 , and finally after autoclaving;

[0033] Mother liquor I is composed of the following substances in parts by weight: ammonium nitrate 900mg / L, potassium dihydrogen phosphate 160mg / L, magnesium sulfate 190mg / L, potassium nitrate 1100mg / L, calcium chloride dihydrate 1100mg / L;

[0034]Mother liquor II is composed of the following substances in parts by weight: copper sulfate pentahydrate 0.04-0.07mg / L, boric acid 6-10mg / L, manganese sulfate 18mg / L, zinc sulfate 10mg / L, cobalt chloride 0.07mg / L, potassium iodide 0.65 mg / L, sodium molybdate 0.45mg / L;

[0035] Mother liquor III is composed of the following materials in parts by weight: 35 mg / L of ferrous sulfate heptahydrate, 55 mg / L of sodium edetate;

[0036] M...

Embodiment 3

[0040] A tissue culture medium for dendrobium officinale seeds, mixing mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V and mother liquor VI according to a volume ratio of 4:1:1:2:2:0.04, and then boiling to adjust pH=6.0 , and finally after autoclaving;

[0041] Mother liquor I is composed of the following substances in parts by weight: ammonium nitrate 825mg / L, potassium dihydrogen phosphate 147mg / L, magnesium sulfate 177mg / L, potassium nitrate 948mg / L, calcium chloride dihydrate 965mg / L;

[0042] Mother liquor II is composed of the following substances in parts by weight: copper sulfate pentahydrate 0.045mg / L, boric acid 7.5mg / L, manganese sulfate 16.5mg / L, zinc sulfate 9.2mg / L, cobalt chloride 0.055mg / L, potassium iodide 0.45mg / L, sodium molybdate 0.28mg / L;

[0043] Mother liquor III is composed of the following materials in parts by weight: 29 mg / L of ferrous sulfate heptahydrate, 38 mg / L of sodium edetate;

[0044] Mother liquor ...

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PUM

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Abstract

The invention relates to the technical field of tissues, in particular to a dendrobium officinale seed tissue culture medium.The culture medium is formed by mixing mother liquor I, mother liquor II, mother liquor III, mother liquor IV, mother liquor V and mother liquor VI, and the volume ratio of the mother liquor I to the mother liquor II to the mother liquor III to the mother liquor IV to the mother liquor V and the mother liquor VI is (3-5): (1-2): (1-2): (1-2): (2-3): (0.03-0.05); the mother liquor VI is composed of, by weight, 5-10 mg / L of metalaxyl, 30-50 mg / L of compound sodium nitrophenolate, 30-80 mg / L of forchior fennron, 15-55 mg / L of microcystin, 30-80 mg / L of brassinosteroid.The dendrobium officinale seed tissue culture medium has the advantages that dendrobium officinale seed cell division can be quickly promoted, the germination time can be accelerated, it can be guaranteed that seedlings grow healthily and are free of diseases, colors are glossy dark green, bud flesh is satiate in thickness, and the bud ratio reaches 100%, and the preparation technology is easy to operate and convenient to popularize.

Description

【Technical field】 [0001] The invention relates to the technical field of tissue culture, in particular to a seed tissue culture medium of Dendrobium officinale. 【Background technique】 [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herb of the genus Dendrobium in the family Orchidaceae. It is listed as the first of the "Nine Immortal Grasses in China" in "Tao Zang". Anti-aging, anti-tumor and other effects. Because of its unique efficacy, Dendrobium candidum has been favored by many health-preserving people since ancient times. Wild Dendrobium candidum is mainly distributed in Zhejiang, Anhui, Guizhou, Jiangxi, Yunnan and other places. Due to its unique structure, the seeds of Dendrobium candidum cannot germinate alone. They can only germinate naturally when they encounter suitable symbiotic bacteria. The wild resources of Dendrobium officinale are now on the verge of extinction due to the frenzied mining of wild resources. [0003] Nowadays, more of...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张小英吴长城
Owner LINGYUN LONGEVITY IMMORTAL GRASS BIOTECH SCI DEV
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